NWNR100029 Practial course in classical analytical chemistry
- Type
- course with continuous assessment
- Semester hours
- 3
- Lecturer (assistant)
- Berthiller, Franz , Steininger-Mairinger, Teresa , Fischer, Elisabeth , Kastner, Bruna , Tchaikovsky, Anastassiya , Steiner-Friedmann, Christina , Luef, Christoph , Kandler, Wolfgang , Hann, Stephan , Vasilieva, Viktoriya , Viehauser, Petra , Sulyok, Michael
- Organisation
- Analytical Chemistry
- Offered in
- Sommersemester 2026
- Languages of instruction
- Deutsch
- Content
-
Classical quantitative analysis (titrimetric analysis) – (Acid–Base titration; complexometric titration)
Determination of ammonium using a Parnas–Wagner apparatus
Conductometry; quantitative instrumental analysis
Gravimetry; quantitative precipitation and possible errors, forms of weighing and precipitation
Photometry; quantitative evaluation using Lambert–Beer law, preparation of calibration standards, calculating results via calibration curve; recording and plotting UV/VIS spectra with a photometer
Glass electrode; working with buffer solutions
Spectroscopy (ICP–AES) using an internal standard and quality control (QC)
Separation methods and mass spectrometry (GC–MS); extraction of plasticizers from plastic samples; difference between SIM and Scan modes
Chromatography (IC), electroanalysis (conductivity detection); verification of analytical methods; preparation of mixed standard solutions; ensuring quality in routine operation (limit of detection, accuracy, precision), producing chromatograms; quadratic regression
Documentation (tabular listing of analytical data and graphical presentation) of analytical results, taking into account significant figures and measurement uncertainty
The following practical examples are covered during lab exercises:
Sample 0: Laboratory plan
Sample 1: Determination of water hardness by acid–base or complexometric titration
Sample 2: Determination of ammonium by Parnas–Wagner vs. titration with conductometric indication
Sample 3: Photometric determination of an indicator’s pKa value
Sample 4: Gravimetric vs. photometric determination of iron concentration
Sample 5: Mineral water analysis using ICP–AES
Sample 6: Ion chromatography for quantitative determination of anions (chloride, nitrate, sulfate)
Sample 7: Qualitative analysis of plasticizers in various everyday plastic items using gas chromatography with mass spectrometry (GC–MS)
- Previous knowledge expected
-
Completion of the NWNR100001 “Introduction to Chemistry” lecture and NWNR100002 “Introduction to Chemistry” tutorials is assumed.
Completion of NWNR100011 “General and Physical Chemistry” tutorials is strongly recommended.
Attendance of the NWNR100028 “Analytical Chemistry” lecture is highly recommended before enrolling in NWNR100029 “Analytical Chemistry” laboratory, as the lab test must be passed successfully to join the lab course.
Basic Excel and statistics skills (mean, standard deviation, creating and labeling charts, t-test, etc.)
Requirements: 771102 Introduction to General Chemistry, Practical Course and 771100 Introduction into General and Analytical Chemistry
Requirements: 771102 Introduction to General Chemistry, Practical Course and 771100 Introduction into General and Analytical Chemistry
- Objective (expected results of study and acquired competences)
-
Upon successful completion, students will be able to:
Perform analyses with required accuracy and evaluate results mathematically and graphically. Record instrumentally obtained measurement data in a lab journal.
Operate and calibrate analytical instruments (pH meter, potentiometer, conductometer, photometer, GC–MS, ICP–AES, IC) and a distillation apparatus.
Properly use piston pipettes and prepare dilutions and calibration series.
Independently perform quantitative titrimetric determinations using conductometric and potentiometric end-point detection.
Carry out extractions for sample preparation independently.
Conduct gravimetric determinations independently and evaluate results; use analytical balances correctly; identify, discuss, and avoid potential weighing errors.
Plan, perform, and document titrimetric determinations, including calculations (molar and mass concentration, titer, standard deviation) and selection of appropriate indicators, ensuring required accuracy (lab journal).
Explain the principle of internal standardization and QC measurements, justify their benefits, and apply them in a quantitative ICP–AES analysis.
Plan, perform, and evaluate quantitative chromatographic and photometric determinations (including calibration).
Formulate qualitative and quantitative results in a comprehensive report (including tabulated data and graphical representation), stating accuracy and measurement uncertainty for qualitative results.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.