BTLW000928 Advanced light and chemical microscopy in life science

Type
Lecture and exercise
Semester hours
2
Lecturer (assistant)
Göhring, Janett
Organisation
Biotechnology and Food Science
Offered in
Wintersemester 2025/26
Languages of instruction
Englisch
Content

The course will give an introduction to Basic Light and Fluorescence Microscopy (fundamental optical principles, as well as sample preparation strategies) and give an introduction to Advanced imaging strategies (single-molecule localization and tracking techniques, super-resolution techniques, as welle as label-free imaging). The course will also cover the design of meaningful experiments for fixed and live specimen. Intermittent exercises in image analysis will be offered for most common analysis strategies (artefact removal, colocalizations, image segmentation, object identification, strategies for time-lapse microscopy).
Previous knowledge expected

Basic knowledge in cell biology is recommended, not obligatory. This course is specifically recommended for Master students interested in Microscopy or for PhD-students who plan to acquire microscopy images from their object of interest.
Objective (expected results of study and acquired competences)

Students will learn the basic principles and applications of microscopy in life sciences and will have increasing confidence in handling basic light and fluorescent microscopes. They will be able to select the appropriate microscope and design an experiment including the proper controls for their individual application. Furthermore, students will be able to identify important aspects of the different techniques, critically interpret results and perform basic troubleshooting. They will understand the basic principles and applications of more advanced microscopical techniques and thereby have the confidence to gain a deeper understanding by consulting the relevant literature on their own.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.