1. Sumesgutner D., Brunmayr P., Gopko V., Dobersberger M. & Traxlmayr M.W. Specific detection of EGF-EGFR complexes on the cell surface and their dependency on EGFR-directed TKI treatment. Biochem Biophys Res Commun. Online ahead of print (2026).
  2. Roefs M.T., Gamauf J., Kroenigsberger B., Brancolini A., Traxlmayr M.W., Arcalis E., Jacak J., van Mechelen M., Prieels J.P., Grillari-Voglauer R., Grillari J. & Bobbili M.R. Rapid extracellular vesicle surface decoration with targeting moieties based on a fluorescein binding single chain variable fragment snorkel. J Control Release. 390:114558 (2026).
  3. Kalinichenko A., Huemer J., Humer T., Haimel M., Svaton M., Socquet-Juglard N., Perinetti Casoni G., Prakash C., von der Linde M., Guthrie J., van de Wetering C., Nunez-Fontarnau J., Kamnev A., Giuliani S., Jaeger M.G., Hahn E., Dobner S., Rukavina A., Sylvander E., Seigner J., Rashkova C., Hoeger B., Hannich J., Traxlmayr M.W., Lehner M., Bryceson Y.T., Saarela J., Castanon I., Winter G., Dupré L. & Boztug K. Protein palmitoylation and sphingolipid metabolism control regulated exocytosis in cytotoxic lymphocytes. Sci Immunol. 10(112):eado3825 (2025).
  4. Dianatkhah S., Kogelmann B., Melnik S., Eminger F., Kallolimath S., Sun L., Sumesgutner D., Traxlmayr M.W., Sack M., Stoger E., Steinkellner H. A plant cell-based platform for the expression of complex proteins with fucose-reduced sialylated N-glycans. Plant Biotechnol J. Online ahead of print (2025).
  5. Zajc C.U., Sylvander E., Teufl M. & Traxlmayr M.W. Protein Engineering by Yeast Surface Display. J Vis Exp. 213 (2024).
  6. Dobersberger M., Sumesgutner D., Zajc C.U. & Traxlmayr M.W. Protocol for engineering binding domains to recognize ligand-bound receptors by using yeast surface display. STAR Protoc. 5(4), 103339 (2024).
  7. Zajc C.U., Sylvander E., Lehner M. & Traxlmayr M.W. Small molecule-regulated switches to provide functional control of CAR T cells within the patient. Expert Opin Biol Ther. 24(6), 425-432 (2024).
  8. Dobersberger M., Sumesgutner D., Zajc C.U., Salzer B., Laurent E., Emminger D., Sylvander E., Lehner E., Teufl M., Seigner J., Bobbili M.R., Kunert R., Lehner M. & Traxlmayr M.W. An engineering strategy to target activated EGFR with CAR T cells. Cell Rep Methods. 4, 100728 (2024).
  9. Seigner J., Zajc C.U., Dötsch S., Eigner C., Laurent E., Busch D.H., Lehner M. & Traxlmayr M.W. Solving the mystery of the FMC63-CD19 affinity. Sci Rep. 13(1), 23024 (2023).
  10. Wagner A., Galicia-Andrés E., Teufl M., Gold L., Obinger C., Sykacek P., Oostenbrink C. & Traxlmayr M.W. Identification of activating mutations in the transmembrane and extracellular domains of EGFR. Biochemistry.61(19), 2049-2062 (2022).
  11. Zajc C.U., Teufl M. & Traxlmayr M.W. Affinity and Stability Analysis of Yeast Displayed Proteins. Methods Mol Biol. 2491, 155-173 (2022).
  12. Teufl M., Zajc C.U. & Traxlmayr M.W. Engineering Strategies to Overcome the Stability-Function Trade-Off in Proteins. ACS Synth Biol. 11(3), 1030-1039 (2022).
  13. Wagner A., Teufl M., Gold L., Lehner M., Obinger C., Sykacek P. & Traxlmayr M.W. PhosphoFlowSeq - A High-throughput Kinase Activity Assay for Screening Drug Resistance Mutations in EGFR. J Mol Biol. 433(22), 167210 (2021).
  14. Paumann-Page M., Kienzl N.F., Motwani J., Bathish B., Paton L.N., Magon N.J., Sevcnikar B., Furtmüller P.G., Traxlmayr M.W., Obinger C., Eccles M.R. & Winterbourn C.C. Peroxidasin protein expression and enzymatic activity in metastatic melanoma cell lines are associated with invasive potential. Redox Biol. 46, 102090 (2021).
  15. Laurent E., Sieber A., Salzer B., Wachernig A., Seigner J., Lehner M., Geyeregger R., Kratzer B., Jäger U., Kunert R., Pickl W.F. & Traxlmayr M.W. Directed Evolution of Stabilized Monomeric CD19 for Monovalent CAR Interaction Studies and Monitoring of CAR-T Cell Patients. ACS Synth Biol. 10(5), 1184-1198 (2021).
  16. Zajc C.U., Salzer B., Taft J.M., Reddy S.T., Lehner M.* & Traxlmayr M.W.* Driving CARs with alternative navigation tools – the potential of engineered binding scaffolds. FEBS J. 288(7), 2103-2118 (2021). *Corresponding authors
  17. Herzog P.L., Borghi E., Traxlmayr M.W., Obinger C., Sikes H.D. & Peterbauer C.K. Developing a cell-bound detection system for the screening of oxidase activity using the fluorescent peroxide sensor roGFP2-Orp1. Protein Eng Des Sel. 33, gzaa019 (2020).
  18. Salzer B., Schueller C.M., Zajc C.U., Peters T., Schoeber M.A., Kovacic B., Buri M.C., Lobner E., Dushek O., Huppa J.B., Obinger C., Putz E.M., Holter W., Traxlmayr M.W.* & Lehner M.* Engineering AvidCARs for combinatorial antigen recognition and reversible control of CAR function. Nat Commun. 11(1), 4166 (2020). *Corresponding authors
  19. Zajc C.U., Dobersberger M., Schaffner I., Mlynek G., Pühringer D., Salzer B., Djinovic-Carugo K., Steinberger P., De Sousa Linhares A., Yang N.J., Obinger C., Holter W., Traxlmayr M.W.* & Lehner M.* A conformation-specific ON-switch for controlling CAR T cells with an orally available drug. Proc Natl Acad Sci U S A. 117(26), 14926-14935 (2020). *These authors contributed equally to this work
  20. Lobner E., Humm A.S., Mlynek G., Kubinger K., Kitzmüller M., Traxlmayr M.W., Djinović-Carugo K. & Obinger C. Two-faced Fcab prevents polymerization with VEGF and reveals thermodynamics and the 2.15 Å crystal structure of the complex. mAbs. 9(7), 1088-1104 (2017).
  21. Kauke M.J.*, Traxlmayr M.W.*, Parker J.A.*, Kiefer J.D., Knihtila R., McGee J., Verdine G., Mattos C. & Wittrup K.D. An engineered protein antagonist of K-Ras/B-Raf interaction. Sci Rep. 7(1), 5831 (2017). *These authors contributed equally to this work
  22. Yang N.J., Kauke M.J., Sun F., Yang L.F., Maass K.F., Traxlmayr M.W., Yu Y., Xu Y., Langer R.S., Anderson D.G. & Wittrup K.D. Cytosolic delivery of siRNA by ultra-high affinity dsRNA binding proteins. Nucleic Acids Res. 45(13), 7602-7614 (2017).
  23. Lobner E., Humm A.S., Göritzer K., Mlynek G., Puchinger M.G., Hasenhindl C., Rüker F., Traxlmayr M.W., Djinović-Carugo K. & Obinger C. Fcab-HER2 Interaction: a Ménage à Trois. Lessons from X-Ray and Solution Studies. Structure. 25(6), 878-889.e5 (2017).
  24. Traxlmayr M.W.* & Shusta E.V. Directed Evolution of Protein Thermal Stability Using Yeast Surface Display. Methods Mol Biol. 1575, 45-65 (2017). *Corresponding author
  25. Miller E.A., Traxlmayr M.W., Shen J. & Sikes H.D. Activity-based assessment of an engineered hyperthermophilic protein as a capture agent in paper-based diagnostic tests. Mol Syst Des Eng. 1(4), 377-381 (2016).
  26. Traxlmayr M.W.*, Kiefer J.D., Srinivas R.R., Lobner E., Tisdale A.W., Mehta N.K., Yang N.J., Tidor B. & Wittrup K.D.* Strong Enrichment of Aromatic Residues in Binding Sites from a Charge-neutralized Hyperthermostable Sso7d Scaffold Library. J Biol Chem. 291(43), 22496-22508 (2016). *Corresponding authors
  27. de Picciotto S., Dickson P.M., Traxlmayr M.W., Marques B.S., Socher E., Zhao S., Cheung S., Kiefer J.D., Wand A.J., Griffith L.G., Imperiali B. & Wittrup K.D. Design Principles for SuCESsFul Biosensors: Specific Fluorophore/Analyte Binding and Minimization of Fluorophore/Scaffold Interactions. J Mol Biol. 428(20), 4228-4241 (2016).
  28. Lobner E., Traxlmayr M.W., Obinger C. & Hasenhindl C. Engineered IgG1-Fc – one fragment to bind them all. Immunol Rev. 270(1), 113-31 (2016).
  29. Angelini A., Chen T.F., de Picciotto S., Yang N.J., Tzeng A., Santos M.S., Van Deventer J.A., Traxlmayr M.W. & Wittrup K.D. Protein Engineering and Selection Using Yeast Surface Display. Methods Mol Biol. 1319, 3-36 (2015).
  30. Traxlmayr M.W., Lobner E., Hasenhindl C., Stadlmayr G., Oostenbrink C., Rüker F. & Obinger C. Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution. Biotechnol J. 9(8), 1013-1022 (2014).
  31. Hasenhindl C., Lai B., Delgado J., Traxlmayr M.W., Stadlmayr G., Rüker F., Serrano L., Oostenbrink C. & Obinger C. Creating stable stem regions for loop elongation in Fcabs - insights from combining yeast surface display, in silico loop reconstruction and molecular dynamics simulations. Biochim Biophys Acta. 1844(9), 1530-40 (2014).
  32. Hasenhindl C., Traxlmayr M.W., Wozniak-Knopp G., Jones P.C., Stadlmayr G., Rüker F. & Obinger C. Stability assessment on a library scale: a rapid method for the evaluation of the commutability and insertion of residues in C-terminal loops of the CH3 domains of IgG1-Fc. Protein Eng Des Sel. 26(10), 675-82 (2013).
  33. Traxlmayr M.W., Lobner E., Antes B., Kainer M., Wiederkum S., Hasenhindl C., Stadlmayr G., Rüker F., Woisetschläger M., Moulder K. & Obinger C. Directed evolution of Her2/neu-binding IgG1-Fc for improved stability and resistance to aggregation by using yeast surface display. Protein Eng Des Sel. 26(4), 255-265 (2013).
  34. Traxlmayr M.W., Hasenhindl C., Hackl M., Stadlmayr G., Rybka J.D., Borth N., Grillari J., Rüker F. & Obinger C. Construction of a stability landscape of the CH3 domain of human IgG1 by combining directed evolution with high throughput sequencing. J Mol Biol. 423(3), 397-412 (2012).
  35. Traxlmayr M.W. & Obinger C. Directed evolution of proteins for increased stability and expression using yeast display. Arch Biochem Biophys. 526(2), 174-180 (2012).
  36. Traxlmayr M.W., Faissner M., Stadlmayr G., Hasenhindl C., Antes B., Rüker F. & Obinger C. Directed evolution of stabilized IgG1-Fc scaffolds by application of strong heat shock to libraries displayed on yeast. Biochim Biophys Acta. 1824(4), 542-9 (2012).
  37. Traxlmayr M.W., Wozniak-Knopp G., Antes B., Stadlmayr G., Rüker F. & Obinger C. Integrin binding human antibody constant domains – probing the C-terminal structural loops for grafting the RGD motif.J Biotechnol. 155(2), 193-202 (2011).
  38. Traxlmayr M.W., Wesch D., Dohnal A.M., Funovics P., Fischer M.B., Kabelitz D. & Felzmann T. Immune suppression by gammadelta T-cells as a potential regulatory mechanism after cancer vaccination with IL-12 secreting dendritic cells. J Immunother. 33(1), 40-52 (2010).