• Pichia pastoris

    Pichia pastoris

    a host for recombinant protein production

Molecular Biotechnology determines and describes the underlying molecular principles of biotechnological production processes. Among these products, recombinant proteins (e.g. biotherapeutics and industrial enzymes) are of paramount importance. To accelerate the development of production strains it is crucial to understand the molecular physiology of the host, and the specific limitations that the product may exert on expression.

The yeast Pichia pastoris (syn. Komagataella spp) has emerged as an efficient host for recombinant protein production. We focus on the comprehensive understanding of protein folding and secretion, and on cell engineering for the improvement of these processes (BioTop Project 2019; BioTop Project 2015). Furthermore, we are part of the MSCA-ITN SECRETERS which designs new-generation microbial platforms for the production of disulphide-bonded and 'difficult-to-express' proteins (http://secreters-msca-itn.eu/).

In the “CD-Laboratory for growth-decoupled protein production in yeast”, the connection between cell growth and protein secretion is analyzed by looking at yeast cells cultivated at very low specific growth rates, using the yeast P. pastoris as a model system (link).

Systems biology analyses include genome, transcriptome, proteome, metabolome, and fluxome analyses, and metabolic modelling, these high throughput technologies are performed in cooperation with the Department of ChemistryFH Campus Wien Bioinformatics group, and VBCF. Computational metabolic modelling is performed in joint projects with Metabolic Modelling group. Curated genome sequences of different P. pastoris strains are available at www.pichiagenome.org.

Pichia Genome Database

This database hosts up-to-date annotation information of the genome of the methylotrophic yeast Pichia pastoris (Komagataella spp.), containing the new manually achieved annotation of K. phaffii strain CBS7435, and basic sequence and annotation information taken from the GenBank data of various Komagataella strains and species.
Additionally the sequence information of S. cerevisiae strain S288c is also available for comparison and homolog display.

Latest Publication

Synthetic activation of yeast stress response improves secretion of recombinant proteins

(2023) Zahrl RJ, Prielhofer R, Burgard J, Mattanovich D, Gasser B. N Biotechnol. 2023 Mar 25;73:19-28. doi: 10.1016/j.nbt.2023.01.001.

BOKU Töchtertag 2022

Workshop: Lebende Proteinfabriken: von der Zelle zum Produkt
Hefen und andere Mikroorganismen werden häufig zur Herstellung von wichtigen Nahrungsmitteln und Medikamenten verwendet. Beispiele dafür sind Brot und Backwaren, Bier, Wein sowie Medikamente zur Behandlung von Diabetes (Insulin) und Impfstoffe (zB gegen Hepatitis B oder HPV). Im Workshop werden wir folgende Experimente gemeinsam durchführen: Mikroorganismen und Zellen im Mikroskop betrachten, Genetische Information (DNA) aus Lebensmitteln extrahieren und/oder Proteingehalt in Lebensmitteln bestimmen. Mit fachlicher Einleitung und Unterstützung von Brigitte Gasser wurde den interessierten Mädchen die Welt der Mikrooorganismen spannend näher gebracht!

Two homologs of the Cat8 transcription factor are involved in the regulation of ethanol utilization in Komagataella phaffii

Barbay, D; Mačáková, M; Sützl, L; De, S; Mattanovich, D; Gasser, B. (2021) Curr Genet. 2021; 67(4): 641–661. Published online 2021 Mar 16. doi: 10.1007/s00294-021-01165-4

Pushing and pulling proteins into the yeast secretory pathway enhances recombinant protein secretion

(2022) Zahrl RJ, Prielhofer J, Ata Ö, Baumann K, Mattanovich D, Gasser B. Metab Eng. 2022 Nov;74:36-48. doi: 10.1016/j.ymben.2022.08.010.

FEMS Yeast Research Best Article Award 2021

What makes Komagataella phaffii non-conventional?

(2021) Ata Ö, Ergün BG, Fickers P, Heistinger L, Mattanovich D, Rebnegger C, Gasser B. FEMS Yeast Res. 2021 Dec 24;21(8):foab059. doi: 10.1093/femsyr/foab059. PMID: 34849756; PMCID: PMC8709784.

BOKU Erfinderin 2019

Brigitte Gasser wurde 2019 als eine der BOKU Erfinderinnen 2019 gewürdigt.

In diesem Video stellt sie sich selbst und ihre Forschungsarbeit vor.

BOKU Invention Awards 2019 for Brigitte Gasser, Diethard Mattanovich, Michael Sauer and Thomas Gaßler

The inventors, Diethard Mattanovich, Brigitte Gasser, Michael Sauer and Thomas Gaßler were able to convince the jury with the "CarboFeed" technology. In this project, a method was developed that makes it possible to process CO2 into an animal feed additive by using a novel yeast. The aim is using CO2 as a sustainable source of raw materials and it also has the eco-friendly effect that no further agricultural land is needed for the production of animal feed.

Brigitte Gasser, Assoc. Prof. Priv.-Doz. Dipl.-Ing. Dr.nat.techn.

H79200 Institut für Mikrobiologie und Mikrobielle Biotechnologie

+43 1 47654-79033
+43 1 47654-79009
Institut für Mikrobiologie und Mikrobielle Biotechnologie
Muthgasse 18/V
1190 Wien
Muthgasse 18/IV
1190 Wien