Mattanovich Lab: Metabolic and Cell Engineering

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We study metabolism, cell biology and biodiversity of methylotrophic yeasts. Yeasts of the Komagataella genus (formerly known as Pichia pastoris) entered the stage as bioproduction hosts, however they also make excellent models to study non canonical metabolic pathways, evolution of metabolism and species formation. We engineer their metabolism to enable the utilization of alternative substrates (even carbon dioxide) or the production of new compounds like organic acids and heterologous proteins and we develop tailored production processes. In addition we study the genetic diversity of the Komagataella genus, and investigate the regulation of carbon metabolism.

Metabolic Engineering

Cell & Process Engineering

Genome Diversity

Pichia Genome Database

This database hosts up-to-date annotation information of the genome of the methylotrophic yeast Pichia pastoris (Komagataella spp.), containing the new manually achieved annotation of K. phaffii strain CBS7435, and basic sequence and annotation information taken from the GenBank data of various Komagataella strains and species.
Additionally the sequence information of S. cerevisiae strain S288c is also available for comparison and homolog display.

Zur Applikation

http://www.pichiagenome.org

Publications of this Research Group

Michael Baumschabl received the OeGMBT PhD Award 2024

Congratulations to Michael Baumschabl, who has been awarded the prestigious PhD Award at this year’s ÖGMBT conference for his outstanding dissertation. His research significantly improved the synthetic autotrophic strain Komagataella phaffii, which uses CO2 as its sole carbon source, enabling it to produce up to 600 mg/L of lactic acid. Additionally, he discovered a natural recycling pathway for the toxic byproduct 2-phosphoglycolate through the deletion of the CYB2 gene.

Video über Forschungserfolge

3 Fragen an Diethard Mattanovich

erstellt vom BOKU Team für Öffentlichkeitsarbeit

Fermenting Futures: an artistic view on yeast biotechnology

(2021) Dumitriu, A, May, A, Ata, Ö, Mattanovich, D. FEMS Yeast Res. 2021 Aug 5;21(5):foab042. doi: 10.1093/femsyr/foab042.

Overexpression of the transcriptional activators Mxr1 and Mit1 enhances lactic acid production on methanol in Komagataellaphaffii

Bachleitner S, Severinsen MM, Lutz G, Mattanovich D. 2024 - Metab Eng. 2024 Sep;85:133-144. doi: 10.1016/j.ymben.2024.07.013. Epub 2024 Jul 25. PMID: 39067842.

Single carbon metabolism - A new paradigm for microbial bioprocesses?

Baumschabl M, Ata Ö, Mattanovich D.2024 - Synth Syst Biotechnol. 2024 Mar 11;9(2):322-329. doi: 10.1016/j.synbio.2024.03.003. PMID: 38545459; PMCID: PMC10965485.

Latest Publication

Tailored extraction and ion mobility-mass spectrometry enables isotopologue analysis of tetrahydrofolate vitamers

(2023) Mitic B, Mattanovich D, Hann S, Causon, T. Anal Bioanal Chem. 2023 Sep;415(21):5151-5163. doi: 10.1007/s00216-023-04786-5.

Diethard Mattanovich's Lectures

Efficient production of itaconic acid from the single-carbon substrate methanol with engineered Komagataella phaffii

Severinsen MM, Bachleitner S, Modenese V, Ata Ö, Mattanovich D. 2024 - Biotechnol Biofuels Bioprod. 2024 Jul 15;17(1):98. doi: 10.1186/s13068-024-02541-1. PMID: 39010147; PMCID: PMC11251334.

Book release

Recombinant Protein Production in Yeast

This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. in: Methods Mol Biol. 2019;1923: ISBN-13: 978-1493990238

Diethard Mattanovich, Univ.Prof. Dipl.-Ing. Dr.nat.techn.

Departmentleiter: H79000 Department für Biotechnologie (DBT)
Leiter: H79200 Institut für Mikrobiologie und Mikrobielle Biotechnologie

Email: diethard.mattanovich@boku.ac.at
Telefon: +43 1 47654-79006
Fax: +43 1 47654-79009
Postadresse: Institut für Mikrobiologie und Mikrobielle Biotechnologie
Muthgasse 18/V
1190 Wien
Büro: Muthgasse 18
1190 Wien

Weiterführende Links

Publications