Enhanced Lignin Nanoparticle Size Characterization Using Asymmetric Flow Field-Flow Fractionation

Size and shape characterization of lignin nanoparticles (LNPs) are crucial factors in their successful valorization. At CF Alice, we offer a state-of-the-art method utilizes asymmetric flow field-flow fractionation (AF4) combined with multi-angle light scattering (MALS), dynamic light scattering (DLS), and refractive index (RI) detection to deliver high-resolution particle size distributions. This method enhances accuracy compared to traditional batch DLS and atomic force microscopy (AFM), providing deeper insights into LNP dispersity and stability. Additionally, we analyze the impact of particle size on MALS and DLS signals and determine the shape factor (ρ) of LNPs. With minimal sample preparation, high precision, and broad applicability, our AF4-based method is ideal for researchers and industries seeking reliable nanoparticle characterization.

References

Kohlhuber, N., Sulaeva, I., Zou, T., Musl, O., Mildner, R., Renneckar, S., Österberg, M., Rosenau, T., Potthast, A. (2025).
Advanced Characterization of Lignin Nanoparticles by Asymmetric Flow-Field Flow Fractionation.
ChemSusChem, e202500329. doi: 10.1002/cssc.202500329

DLS for nanoparticle sizing

Dynamic Light Scattering (DLS) is a technique that analyzes fluctuations in scattered light intensity caused by the random, thermal motion (Brownian motion) of particles suspended in a liquid. These fluctuations are used to calculate the diffusion coefficient, which is then used to derive the hydrodynamic radius (Rh) of the particles. Operating in batch mode (i.e., without flow), DLS can measure a wide particle size range—from as small as 0.2 nm up to 5000 nm in radius, making it a powerful tool for particle size characterization across various applications, including lignin-based nanoparticles.

References

Striegel, A.M., Podzimek, S. (2012)
Light scattering, size exclusion chromatography and asymmetric flow field flow fractionation. Powerful tools for the characterization of polymers, proteins and nanoparticles.
Anal Bioanal Chem, 402, 1857–1858. doi.org/10.1007/s00216-011-5607-x

Kohlhuber, N., Sulaeva, I., Zou, T., Musl, O., Mildner, R., Renneckar, S., Österberg, M., Rosenau, T., Potthast, A. (2025).
Advanced Characterization of Lignin Nanoparticles by Asymmetric Flow-Field Flow Fractionation.
ChemSusChem, e202500329. doi: 10.1002/cssc.202500329