Publications

Leitgeb U., Crha R., Fegerl I., Furtmüller P. G., Oostenbrink C., and Pfanzagl V.
Halide binding by myeloperoxidase is regulated by access channel dynamics and charge interactions.
International Journal of Biological Macromolecules, 2025. 330: 148038.

Dali A., Gabler T., Sebastiani F., Furtmüller P. G., Becucci M., Hofbauer S., and Smulevich G.
Entrance channels to coproheme in coproporphyrin ferrochelatase probed by exogenous imidazole binding.
Journal of Inorganic Biochemistry, 2024. 260: 112681.

Rossmueller G., Mirkina I., Thiele M., Puchol Tarazona A., Rueker F., Kerschbaumer R. J., and Schinagl A.
Integrating In Silico and In Vitro Tools for Optimized Antibody Development-Design of Therapeutic Anti-oxMIF Antibodies.
Antibodies, 2024. 13(4): 104.

Dali A., Gabler T., Sebastiani F., Destinger A., Furtmüller P. G., Pfanzagl V., Becucci M., Smulevich G., and Hofbauer S.
Active site architecture of coproporphyrin ferrochelatase with its physiological substrate coproporphyrin III: Propionate interactions and porphyrin core deformation.
Protein Science, 2023. 32(1): e4534.

Gabler T., Dali A., Sebastiani F., Furtmüller P. G., Becucci M., Hofbauer S., and Smulevich G.
Iron insertion into coproporphyrin III-ferrochelatase complex: Evidence for an intermediate distorted catalytic species.
Protein Science, 2023. 32(11): e4788.

Pfanzagl V., Gruber-Grünwald C., Leitgeb U., Furtmüller P. G., and Obinger C.
Posttranslational modification and heme cavity architecture of human eosinophil peroxidase-insights from first crystal structure and biochemical characterization.
Journal of Biological Chemistry, 2023. 299(12).

Leitgeb U., Furtmüller P. G., Hofbauer S., Brito J. A., Obinger C., and Pfanzagl V.
The staphylococcal inhibitory protein SPIN binds to human myeloperoxidase with picomolar affinity but only dampens halide oxidation.
Journal of Biological Chemistry, 2022. 298(11).

Michlits H., Valente N., Mlynek G., and Hofbauer S.
Initial Steps to Engineer Coproheme Decarboxylase to Obtain Stereospecific Monovinyl, Monopropionyl Deuterohemes.
Frontiers in Bioengineering and Biotechnology, 2022. 9: 807678.

De Mathia F., Kargl T., Müller M., Erdem I., Hayes B., Puente-Massaguer E., Krammer F., and Lingg N.
Purification and characterization of recombinant neuraminidase as a potentially broadly protective influenza virus vaccine candidate.
Vaccine, 2025. 62: 127471.

Natale V., Heves G., Stadlbauer K., Rüker F., Siegmund V., Pekar L., Zielonka S., Toleikis L., Becker S., and Wozniak-Knopp G.
Trispecific SEED antibodies engineered for neutrophil-mediated cell killing.
mAbs, 2025. 17(1): 2532851.

Sun L., Palt R., Schütz G., Föderl-Höbenreich E., Brod L., Hermle A., Lux A., Steinkellner H., and Kallolimath S.
Light Chain Isotype and Antibody-Specificity Impact on Virus Neutralization.
Antibodies, 2025. 14(2).

Teufl M., Mayer J., Holzer K. E., Emminger D., Sylvander E., Baik H., Schubert F., Maniaki E., Bader U., Muñoz-López Á., Mittelstaet J., Dobersberger M., Buri M. C., Sützl L., Salzer B., Putz E. M., Zajc C. U., Lehner M., and Traxlmayr M. W.
MiniCARbids: Minimalistic human binding domains specifically tailored to CAR T applications.
bioRxiv, 2025.

Beitl K. N., and Reimhult E.
Effect of Solvent Properties on the Critical Solution Temperature of Thermoresponsive Polymers.
International Journal of Molecular Sciences, 2024. 25(14): 7734.

Dobersberger M., Sumesgutner D., Zajc C. U., Salzer B., Laurent E., Emminger D., Sylvander E., Lehner E., Teufl M., Seigner J., et al.
An engineering strategy to target activated EGFR with CAR T cells.
Cell Reports Methods, 2024. 4(4).

Gabler T., Sebastiani F., Helm J., Dali A., Obinger C., Furtmüller P. G., Smulevich G., and Hofbauer S.
Substrate specificity and complex stability of coproporphyrin ferrochelatase is governed by hydrogen-bonding interactions of the four propionate groups.
The FEBS Journal, 2022. 289(6): 1680–1699.

Natale V., Stadlmayr G., Benedetti F., Stadlbauer K., Rüker F., and Wozniak-Knopp G.
Trispecific antibodies produced from mAb2 pairs by controlled Fab-arm exchange.
Biological Chemistry, 2022. 403(5-6): 509–523.

Serra I., Schmidt D., Pfanzagl V., Mlynek G., Hofbauer S., Djinović-Carugo K., Furtmüller P. G., Garcìa-Rubio I., Van Doorslaer S., and Obinger C.
Impact of the dynamics of the catalytic arginine on nitrite and chlorite binding by dimeric chlorite dismutase.
Journal of Inorganic Biochemistry, 2022. 227: 111689.

Stadlmayr G., Stracke F., Stadlbauer K., Rybka J., Dickgiesser S., Rasche N., Becker S., Toleikis L., Rüker F., and Knopp G. W.
Efficient spontaneous site-selective cysteine-mediated toxin attachment within a structural loop of antibodies.
Biochimica et Biophysica Acta (BBA)-General Subjects, 2022. 1866(7): 130155.

Benedetti F., Stracke F., Stadlmayr G., Stadlbauer K., Rüker F., and Wozniak-Knopp G.
Bispecific antibodies with Fab-arms featuring exchanged antigen-binding constant domains.
Biochemistry and Biophysics Reports, 2021. 26: 100959.

Geiss A. F., Reichhart T. M., Pejker B., Plattner E., Herzog P. L., Schulz C., Ludwig R., Felice A. K., and Haltrich D.
Engineering the turnover stability of cellobiose dehydrogenase toward long-term bioelectronic applications.
ACS Sustainable Chemistry & Engineering, 2021. 9(20): 7086–7100.

Kotov V., Mlynek G., Vesper O., Pletzer M., Wald J., Teixeira-Duarte C. M., Celia H., Garcia-Alai M., Nussberger S., Buchanan S. K., et al.
In-depth interrogation of protein thermal unfolding data with MoltenProt.
Protein Science, 2021. 30(1): 201–217.

Schmidt D., Serra I., Mlynek G., Pfanzagl V., Hofbauer S., Furtmüller P. G., Djinović-Carugo K., Van Doorslaer S., and Obinger C.
Arresting the catalytic arginine in chlorite dismutases: impact on heme coordination, thermal stability, and catalysis.
Biochemistry, 2021. 60(8): 621–634.

Willinger M., and Reimhult E.
Thermoresponsive nanoparticles with cyclic-polymer-grafted shells are more stable than with linear-polymer-grafted shells: effect of polymer topology, molecular weight, and core size.
The Journal of Physical Chemistry B, 2021. 125(25): 7009–7023.

Sádio F., Stadlmayr G., Stadlbauer K., Gräf M., Scharrer A., Rüker F., and Wozniak-Knopp G.
Stabilization of soluble high-affinity T-cell receptor with de novo disulfide bonds.
FEBS letters, 2020. 594(3): 477–490.

Hofbauer S., Helm J., Obinger C., Djinović-Carugo K., and Furtmüller P. G.
Crystal structures and calorimetry reveal catalytically relevant binding mode of coproporphyrin and coproheme in coproporphyrin ferrochelatase.
The FEBS journal, 2020. 287(13): 2779–2796.

Puchol Tarazona A. A., Lobner E., Taubenschmid Y., Paireder M., Torres Acosta J. A., Göritzer K., Steinkellner H., and Mach L.
Steric accessibility of the cleavage sites dictates the proteolytic vulnerability of the anti-HIV-1 antibodies 2F5, 2G12, and PG9 in plants.
Biotechnology Journal, 2020. 15(3): 1900308.

Schroffenegger M., Leitner N. S., Morgese G., Ramakrishna S. N., Willinger M., Benetti E. M., and Reimhult E.
Polymer topology determines the formation of protein corona on core-shell nanoparticles.
ACS nano, 2020. 14(10): 12708–12718.

Tan S., Abas M., Verstraeten I., Glanc M., Molnár G., Hajnỳ J., Lasák P., Petřı́k I., Russinova E., Petrášek J., et al.
Salicylic acid targets protein phosphatase 2A to attenuate growth in plants.
Current Biology, 2020. 30(3): 381–395.

Zajc C. U., Dobersberger M., Schaffner I., Mlynek G., Pühringer D., Salzer B., Djinović-Carugo K., Steinberger P., De Sousa Linhares A., Yang N. J., et al.
A conformation-specific ON-switch for controlling CAR T cells with an orally available drug.
Proceedings of the National Academy of Sciences, 2020. 117(26): 14926–14935.

Göritzer K., Turupcu A., Maresch D., Novak J., Altmann F., Oostenbrink C., Obinger C., and Strasser R.
Distinct Fc alpha receptor N-glycans modulate the binding affinity to immunoglobulin A (IgA) antibodies.
Journal of Biological Chemistry, 2019. 294(38): 13995–14008.

Laurent C. V., Sun P., Scheiblbrandner S., Csarman F., Cannazza P., Frommhagen M., van Berkel W. J., Oostenbrink C., Kabel M. A., and Ludwig R.
Influence of lytic polysaccharide monooxygenase active site segments on activity and affinity.
International Journal of Molecular Sciences, 2019. 20(24): 6219.

Schwaigerlehner L., Mayrhofer P., Diem M., Steinfellner W., Fenech E., Oostenbrink C., and Kunert R.
Germinality does not necessarily define mAb expression and thermal stability.
Applied microbiology and biotechnology, 2019. 103: 7505–7518.

Schroffenegger M., Zirbs R., Kurzhals S., and Reimhult E.
The role of chain molecular weight and hofmeister series ions in thermal aggregation of poly (2-isopropyl-2-oxazoline) grafted nanoparticles.
Polymers, 2018. 10(4): 451.

Schroffenegger M., and Reimhult E.
Thermoresponsive core-shell nanoparticles: does core size matter?.
Materials, 2018. 11(9): 1654.

Vogt S., Stadlmayr G., Stadlbauer K., Sádio F., Andorfer P., Grillari J., Rüker F., and Wozniak-Knopp G.
Stabilization of the CD81 large extracellular loop with de novo disulfide bonds improves its amenability for peptide grafting.
Pharmaceutics, 2018. 10(3): 138.

Göritzer K., Ruocco V., Vavra U., Izadi S., Bolaños-Martı́nez O. C., Phetphoung T., Pisuttinusart N., Phoolcharoen W., and Strasser R.
Improving the N-glycosylation occupancy of plant-produced IgG1 by engineering the amino acid environment at Asn297.
Frontiers in Plant Science, 2025. 15: 1531710.

Vu-Le K., Phirom-on K., Sripattanakul S., Chu D. B., Hann S., Blaukopf M., Suetzl L., Oostenbrink C., Haltrich D., and Nguyen T.
A GH42 β-Galactosidase from the Human Isolate Bifidobacterium breve DSM 20213: Biochemical and Transgalactosylation Properties Reveal the Potential for Galacto-oligosaccharides Synthesis.
ACS Omega, 2025. 10(51): 62633-62644.

Kostelac A., Hermann E., Peterbauer C., Oostenbrink C., and Haltrich D.
Shifting the substrate scope of dimeric pyranose oxidase from monosaccharide to glycoside preference through oligomeric state modification.
The FEBS Journal, 2025.

Göritzer K., Strasser R., and Ma J. K.
Stability Engineering of Recombinant Secretory IgA.
International Journal of Molecular Sciences, 2024. 25(13): 6856.

Göritzer K., Groppelli E., Grünwald-Gruber C., Figl R., Ni F., Hu H., Li Y., Liu Y., Hu Q., Puligedda R. D., et al.
Recombinant neutralizing secretory IgA antibodies for preventing mucosal acquisition and transmission of SARS-CoV-2.
Molecular Therapy, 2024. 32(3): 689–703.

Spanov B., Olaleye O., Mesurado T., Govorukhina N., Jungbauer A., van de Merbel N. C., Lingg N., and Bischoff R.
Pertuzumab Charge Variant Analysis and Complementarity-Determining Region Stability Assessment to Deamidation.
Analytical Chemistry, 2023. 95(8): 3951–3958.

Izadi S., Gumpelmair S., Coelho P., Duarte H. O., Gomes J., Leitner J., Kunnummel V., Mach L., Reis C. A., Steinberger P., et al.
Plant-derived Durvalumab variants show efficient PD-1/PD-L1 blockade and therapeutically favourable FcR binding.
Plant Biotechnology Journal, 2024. 22(5): 1224–1237.

Ruocco V., Grünwald-Gruber C., Rad B., Tscheliessnig R., Hammel M., and Strasser R.
Effects of N-glycans on the structure of human IgA2.
Frontiers in Molecular Biosciences, 2024. 11: 1390659.

Beihammer G., König-Beihammer J., Kogelmann B., Ruocco V., Grünwald-Gruber C., D'Aoust M., Lavoie P., Saxena P., Gach J. S., Steinkellner H., et al.
An oligosaccharyltransferase from Leishmania donovani increases the N-glycan occupancy on plant-produced IgG1.
Frontiers in Plant Science, 2023. 14.

Izadi S., Vavra U., Melnik S., Grünwald-Gruber C., Föderl-Höbenreich E., Sack M., Zatloukal K., Glössl J., Stöger E., Mach L., et al.
In planta deglycosylation improves the SARS-CoV-2 neutralization activity of recombinant ACE2-Fc.
Frontiers in Bioengineering and Biotechnology, 2023. 11: 1180044.

Izadi S., Gumpelmair S., Coelho P., Duarte H. O., Gomes J., Leitner J., Kunnummel V., Mach L., Reis C. A., Steinberger P., et al.
Plant-derived Durvalumab variants show efficient PD-1/PD-L1 blockade and therapeutically favourable FcR binding.
Plant Biotechnology Journal, 2023.

Ruocco V., Vavra U., König-Beihammer J., Bolaños O., Kallolimath S., Maresch D., Grünwald-Gruber C., and Strasser R.
Impact of mutations on the plant-based production of recombinant SARS-CoV-2 RBDs.
Frontiers in Plant Science, 2023. 14: 1275228.

Schoberer J., Izadi S., Kierein C., Vavra U., König-Beihammer J., Ruocco V., Grünwald-Gruber C., Castilho A., and Strasser R.
The tobacco GNTI stem region harbors a strong motif for homomeric protein complex formation.
Frontiers in Plant Science, 2023. 14.

Seigner J., Zajc C. U., Dötsch S., Eigner C., Laurent E., Busch D. H., Lehner M., and Traxlmayr M. W.
Solving the mystery of the FMC63-CD19 affinity.
Scientific Reports, 2023. 13(1): 23024.

Spanov B., Olaleye O., Mesurado T., Govorukhina N., Jungbauer A., van de Merbel N. C., Lingg N., and Bischoff R.
Pertuzumab Charge Variant Analysis and Complementarity-Determining Region Stability Assessment to Deamidation.
Analytical Chemistry, 2023. 95(8): 3951–3958.

Strobl M. R., Demir H., Stadlmayr G., Stracke F., Hoelzl R., Bohle B., and Wozniak-Knopp G.
Affinity matters for IgE-blocking activity of allergen-specific antibodies.
Allergy, 2023.

Kittler S., Ebner J., Besleaga M., Larsbrink J., Darnhofer B., Birner-Gruenberger R., Schobesberger S., Akhgar C. K., Schwaighofer A., Lendl B., et al.
Recombinant Protein L: Production, Purification and Characterization of a Universal Binding Ligand.
Journal of Biotechnology, 2022. 359: 108–115.

Nagl M., Haske-Cornelius O., Bauer W., Csarman F., Ludwig R., Nyanhongo G. S., and Guebitz G. M.
Towards a better understanding of synergistic enzyme effects during refining of cellulose fibers.
Carbohydrate Polymer Technologies and Applications, 2022. 4: 100223.

Prattes M., Grishkovskaya I., Hodirnau V., Hetzmannseder C., Zisser G., Sailer C., Kargas V., Loibl M., Gerhalter M., Kofler L., et al.
Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1.
Nature Structural & Molecular Biology, 2022. 29(9): 942–953.

Göritzer K., Goet I., Duric S., Maresch D., Altmann F., Obinger C., and Strasser R.
Efficient N-glycosylation of the heavy chain tailpiece promotes the formation of plant-produced dimeric IgA.
Frontiers in Chemistry, 2020. 8: 346.

Salzer B., Schueller C. M., Zajc C. U., Peters T., Schoeber M. A., Kovacic B., Buri M. C., Lobner E., Dushek O., Huppa J. B., et al.
Engineering AvidCARs for combinatorial antigen recognition and reversible control of CAR function.
Nature Communications, 2020. 11(1): 4166.

Sevcnikar B., Schaffner I., Chuang C. Y., Gamon L., Paumann-Page M., Hofbauer S., Davies M. J., Furtmüller P. G., and Obinger C.
The leucine-rich repeat domain of human peroxidasin 1 promotes binding to laminin in basement membranes.
Archives of Biochemistry and Biophysics, 2020. 689: 108443.

Tan S., Abas M., Verstraeten I., Glanc M., Molnár G., Hajnỳ J., Lasák P., Petřı́k I., Russinova E., Petrášek J., et al.
Salicylic acid targets protein phosphatase 2A to attenuate growth in plants.
Current Biology, 2020. 30(3): 381–395.

Zajc C. U., Dobersberger M., Schaffner I., Mlynek G., Pühringer D., Salzer B., Djinović-Carugo K., Steinberger P., De Sousa Linhares A., Yang N. J., et al.
A conformation-specific ON-switch for controlling CAR T cells with an orally available drug.
Proceedings of the National Academy of Sciences, 2020. 117(26): 14926–14935.

Beyer B., Walch N., Jungbauer A., and Lingg N.
How similar is biosimilar? A comparison of infliximab therapeutics in regard to charge variant profile and antigen binding affinity.
Biotechnology Journal, 2019. 14(4): 1800340.

Göritzer K., Turupcu A., Maresch D., Novak J., Altmann F., Oostenbrink C., Obinger C., and Strasser R.
Distinct Fc alpha receptor N-glycans modulate the binding affinity to immunoglobulin A (IgA) antibodies.
Journal of Biological Chemistry, 2019. 294(38): 13995–14008.

Laurent C. V., Sun P., Scheiblbrandner S., Csarman F., Cannazza P., Frommhagen M., van Berkel W. J., Oostenbrink C., Kabel M. A., and Ludwig R.
Influence of lytic polysaccharide monooxygenase active site segments on activity and affinity.
International Journal of Molecular Sciences, 2019. 20(24): 6219.

Xiao Y., Stegmann M., Han Z., DeFalco T. A., Parys K., Xu L., Belkhadir Y., Zipfel C., and Chai J.
Mechanisms of RALF peptide perception by a heterotypic receptor complex.
Nature, 2019. 572(7768): 270–274.

Plöchl K., and Böttcher T.
The solute-binding proteins DppA1–5 of Pseudomonas aeruginosa have distinct substrate profiles.
Scientific Reports, 2025. 15.

Schandl S., Osondu-Chuka G., Guagliano G., Perak S., Petrini P., Briatico-Vangosa F., Reimhult E., and Guillaume O.
Acetylation of alginate enables the production of inks that mimic the chemical properties of P. aeruginosa biofilm.
Journal of Materials Chemistry B, 2025.

Schandl S., Osondu-Chuka G., Durand-Chatton G., Lemaire L., Franconi F., Sedivy A., Reimhult E., and Ovsianikov A.
Synthetically acetylated alginate is a superior in vitro biofilm model for antibiotic testing showing reduced tobramycin affinity.
European Polymer Journal, 2025. 237: 114176.

Gludovacz E., Schuetzenberger K., Resch M., Tillmann K., Petroczi K., Vondra S., Vakal S., Schosserer M., Virgolini N., Pollheimer J., et al.
Human diamine oxidase cellular binding and internalization in vitro and rapid clearance in vivo are not mediated by N-glycans but by heparan sulfate proteoglycan interactions.
Glycobiology, 2021. 31(4): 444–458.

Roma E., Corsi P., Willinger M., Leitner N. S., Zirbs R., Reimhult E., Capone B., and Gasperi T.
Theoretical and Experimental Design of Heavy Metal-Mopping Magnetic Nanoparticles.
ACS Applied Materials & Interfaces, 2021. 13(1): 1386–1397.

Leitner N. S., Schroffenegger M., and Reimhult E.
Polymer brush-grafted nanoparticles preferentially interact with opsonins and albumin.
ACS Applied Bio Materials, 2020. 4(1): 795–806.

Schroffenegger M., Leitner N. S., Morgese G., Ramakrishna S. N., Willinger M., Benetti E. M., and Reimhult E.
Polymer topology determines the formation of protein corona on core-shell nanoparticles.
ACS nano, 2020. 14(10): 12708–12718.

Stephani M., Picchianti L., Gajic A., Beveridge R., Skarwan E., Sanchez de Medina Hernandez V., Mohseni A., Clavel M., Zeng Y., Naumann C., et al.
A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress.
elife, 2020. 9: e58396.

Zajc C. U., Dobersberger M., Schaffner I., Mlynek G., Pühringer D., Salzer B., Djinović-Carugo K., Steinberger P., De Sousa Linhares A., Yang N. J., et al.
A conformation-specific ON-switch for controlling CAR T cells with an orally available drug.
Proceedings of the National Academy of Sciences, 2020. 117(26): 14926–14935.

Göritzer K., Turupcu A., Maresch D., Novak J., Altmann F., Oostenbrink C., Obinger C., and Strasser R.
Distinct Fc alpha receptor N-glycans modulate the binding affinity to immunoglobulin A (IgA) antibodies.
Journal of Biological Chemistry, 2019. 294(38): 13995–14008.

Natale V., Heves G., Stadlbauer K., Rüker F., Siegmund V., Pekar L., Zielonka S., Toleikis L., Becker S., and Wozniak-Knopp G.
Trispecific SEED antibodies engineered for neutrophil-mediated cell killing.
mAbs, 2025. 17(1): 2532851.

John M. M., Hunjadi M., Hawlin V., Reiser J., and Kunert R.
Interaction Studies of Hexameric and Pentameric IgMs with Serum-Derived C1q and Recombinant C1q Mimetics.
Life, 2024. 14(5): 638.

Kolanovic D., Pasupuleti R., Wallner J., Mlynek G., and Wiltschi B.
Site-Specific Immobilization Boosts the Performance of a Galectin-1 Biosensor.
Bioconjugate Chemistry, 2024. 35(12): 1944–1958.

Rodak A., Stadlbauer K., Bobbili M. R., Smrzka O., Rüker F., and Wozniak Knopp G.
Development of a Cytotoxic Antibody-Drug Conjugate Targeting Membrane Immunoglobulin E-Positive Cells.
International Journal of Molecular Sciences, 2023. 24(19): 14997.

Sauerwein K. M., Geier C. B., Stemberger R. F., Rossmanith R., Akyaman H., Illes P., Fischer M. B., Eibl M. M., Walter J. E., and Wolf H. M.
Functionally impaired antibody response to BNT162b2 booster vaccination in CVID IgG responders.
Journal of Allergy and Clinical Immunology, 2023. 151(4): 922–925.

Klausberger M., Kienzl N. F., Stadlmayr G., Grünwald-Gruber C., Laurent E., Stadlbauer K., Stracke F., Vierlinger K., Hofner M., Manhart G., et al.
Designed SARS-CoV-2 receptor binding domain variants form stable monomers.
Biotechnology Journal, 2022. 17(5): 2100422.

Natale V., Stadlmayr G., Benedetti F., Stadlbauer K., Rüker F., and Wozniak-Knopp G.
Trispecific antibodies produced from mAb2 pairs by controlled Fab-arm exchange.
Biological Chemistry, 2022. 403(5-6): 509–523.

Stadlbauer K., Andorfer P., Stadlmayr G., Rüker F., and Wozniak-Knopp G.
Bispecific MAb2 Antibodies Targeting CD59 Enhance the Complement-Dependent Cytotoxicity Mediated by Rituximab.
International Journal of Molecular Sciences, 2022. 23(9): 5208.

Stadlmayr G., Stracke F., Stadlbauer K., Rybka J., Dickgiesser S., Rasche N., Becker S., Toleikis L., Rüker F., and Knopp G. W.
Efficient spontaneous site-selective cysteine-mediated toxin attachment within a structural loop of antibodies.
Biochimica et Biophysica Acta (BBA)-General Subjects, 2022. 1866(7): 130155.

Benedetti F., Stracke F., Stadlmayr G., Stadlbauer K., Rüker F., and Wozniak-Knopp G.
Bispecific antibodies with Fab-arms featuring exchanged antigen-binding constant domains.
Biochemistry and Biophysics Reports, 2021. 26: 100959.

Castilho A., Schwestka J., Kienzl N. F., Vavra U., Grünwald-Gruber C., Izadi S., Hiremath C., Niederhöfer J., Laurent E., Monteil V., et al.
Generation of enzymatically competent SARS-CoV-2 decoy receptor ACE2-Fc in glycoengineered Nicotiana benthamiana.
Biotechnology journal, 2021. 16(6): 2000566.

Gludovacz E., Schuetzenberger K., Resch M., Tillmann K., Petroczi K., Vondra S., Vakal S., Schosserer M., Virgolini N., Pollheimer J., et al.
Human diamine oxidase cellular binding and internalization in vitro and rapid clearance in vivo are not mediated by N-glycans but by heparan sulfate proteoglycan interactions.
Glycobiology, 2021. 31(4): 444–458.

Klausberger M., Duerkop M., Haslacher H., Wozniak-Knopp G., Cserjan-Puschmann M., Perkmann T., Lingg N., Aguilar P. P., Laurent E., De Vos J., et al.
A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests.
EBioMedicine, 2021. 67.

Laurent E., Sieber A., Salzer B., Wachernig A., Seigner J., Lehner M., Geyeregger R., Kratzer B., Jager U., Kunert R., et al.
Directed evolution of stabilized monomeric CD19 for monovalent CAR interaction studies and monitoring of CAR-T cell patients.
ACS synthetic biology, 2021. 10(5): 1184–1198.

Lhota G., Sissolak B., Striedner G., Sommeregger W., and Vorauer-Uhl K.
Quantification of glycated IgG in CHO supernatants: A practical approach.
Biotechnology Progress, 2021. 37(3): e3124.

Schwestka J., König-Beihammer J., Shin Y., Vavra U., Kienzl N. F., Grünwald-Gruber C., Maresch D., Klausberger M., Laurent E., Stadler M., et al.
Impact of specific N-glycan modifications on the use of plant-produced SARS-CoV-2 antigens in serological assays.
Frontiers in Plant Science, 2021. 12: 747500.

Shin Y., König-Beihammer J., Vavra U., Schwestka J., Kienzl N. F., Klausberger M., Laurent E., Grünwald-Gruber C., Vierlinger K., Hofner M., et al.
N-glycosylation of the SARS-CoV-2 receptor binding domain is important for functional expression in plants.
Frontiers in Plant Science, 2021. 12: 689104.

Lobner E., Wachernig A., Gudipati V., Mayrhofer P., Salzer B., Lehner M., Huppa J. B., and Kunert R.
Getting CD19 into shape: expression of natively folded "difficult-to-express" CD19 for staining and stimulation of CAR-T cells.
Frontiers in Bioengineering and Biotechnology, 2020. 8: 49.

Sádio F., Stadlmayr G., Stadlbauer K., Gräf M., Scharrer A., Rüker F., and Wozniak-Knopp G.
Stabilization of soluble high-affinity T-cell receptor with de novo disulfide bonds.
FEBS letters, 2020. 594(3): 477–490.

Schroffenegger M., Leitner N. S., Morgese G., Ramakrishna S. N., Willinger M., Benetti E. M., and Reimhult E.
Polymer topology determines the formation of protein corona on core-shell nanoparticles.
ACS nano, 2020. 14(10): 12708–12718.

Wallner J., Sissolak B., Sommeregger W., Lingg N., Striedner G., and Vorauer-Uhl K.
Lectin bio-layer interferometry for assessing product quality of Fc-glycosylated immunoglobulin G.
Biotechnology progress, 2019. 35(5): e2864.

Göritzer K., Ruocco V., Vavra U., Izadi S., Bolaños-Martı́nez O. C., Phetphoung T., Pisuttinusart N., Phoolcharoen W., and Strasser R.
Improving the N-glycosylation occupancy of plant-produced IgG1 by engineering the amino acid environment at Asn297.
Frontiers in Plant Science, 2025. 15: 1531710.

Göritzer K., Strasser R., and Ma J. K.
Stability Engineering of Recombinant Secretory IgA.
International Journal of Molecular Sciences, 2024. 25(13): 6856.

Göritzer K., Groppelli E., Grünwald-Gruber C., Figl R., Ni F., Hu H., Li Y., Liu Y., Hu Q., Puligedda R. D., et al.
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Herzog P. L., Sützl L., Eisenhut B., Maresch D., Haltrich D., Obinger C., and Peterbauer C. K.
Versatile oxidase and dehydrogenase activities of bacterial pyranose 2-oxidase facilitate redox cycling with manganese peroxidase in vitro.
Applied and Environmental Microbiology, 2019. 85(13): e00390–19.

Berger C., Dietrich W., Richter M., Kellner F., Kühne C., and Strasser K.
Noninvasive Cell Population Profiling of Normal and Dysplastic Cervical Biofluids by Multicolor Flow Cytometry as a Promising Tool for Companion Diagnostics.
Cancers, 2025. 17(20).

Kostopoulou A., Rebnegger C., Ferrero Bordera B., Mattanovich M., Maaß S., Becher D., Gasser B., and Mattanovich D.
Impact of Oxygen Availability on the Organelle-Specific Redox Potentials and Stress in Recombinant Protein Producing Komagataella phaffii.
Microbial Biotechnology, 2025. 18.

Napoleone A., Sebastian I. R., Marco F. D., Molin A., Hussein M., Kramer L., Jostock T., Kelly T., and Borth N.
Overcoming lentiviral delivery limitations in hard-to-transduce suspension cells for genome-wide CRISPR screening.
bioRxiv, 2025.

Sumesgutner D., Brunmayr P., Gopko V., Dobersberger M., and Traxlmayr M. W.
Specific detection of EGF-EGFR complexes on the cell surface and their dependency on EGFR-directed TKI treatment.
Biochemical and Biophysical Research Communications, 2025.: 153165.

Sylvander E., Muñoz-López Á., D'Accardio G., Emminger D., Mouratidis K., Engert F., Baik H., Balaji S., Michls T., Buri M. C., Maresch D., Urbanetz A., Schäfer M., Mittelstaet J., Zuber J., Rosato A., Engels B., Zajc C. U., Traxlmayr M. W., and Lehner M.
Engineering human protein switches for functional control of CARs and transcription factors via oral drug administration.
bioRxiv, 2025.

Teufl M., Mayer J., Holzer K. E., Emminger D., Sylvander E., Baik H., Schubert F., Maniaki E., Bader U., Muñoz-López Á., Mittelstaet J., Dobersberger M., Buri M. C., Sützl L., Salzer B., Putz E. M., Zajc C. U., Lehner M., and Traxlmayr M. W.
MiniCARbids: Minimalistic human binding domains specifically tailored to CAR T applications.
bioRxiv, 2025.

Dobersberger M., Sumesgutner D., Zajc C. U., and Traxlmayr M. W.
Protocol for engineering binding domains to recognize ligand-bound receptors by using yeast surface display.
STAR protocols, 2024. 5(4): 103339.

Dobersberger M., Sumesgutner D., Zajc C. U., Salzer B., Laurent E., Emminger D., Sylvander E., Lehner E., Teufl M., Seigner J., et al.
An engineering strategy to target activated EGFR with CAR T cells.
Cell Reports Methods, 2024. 4(4).

Moldaschl J., Chariyev-Prinz F., Toegel S., Keck M., Hiden U., Egger D., and Kasper C.
Spheroid trilineage differentiation model of primary mesenchymal stem/stromal cells under hypoxia and serum-free culture conditions.
Frontiers in Bioengineering and Biotechnology, 2024. 12: 1444363.

Rebnegger C., Flores-Villegas M., Kowarz V., De S., Pusterla A., Holm H., Adelantado N., Kiziak C., Mattanovich D., and Gasser B.
Knock-out of the major regulator Flo8 in Komagataella phaffii results in unique host strain performance for methanol-free recombinant protein production.
New Biotechnology, 2024. 84: 105–114.

Rebnegger C., Coltman B. L., Kowarz V., Peña D. A., Mentler A., Troyer C., Hann S., Schöny H., Koellensperger G., Mattanovich D., et al.
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Microbial Cell Factories, 2024. 23(1): 43.

Yang G., Schmid-Siegel M., Heissenberger C., Kos-Braun I. C., Prechtl M., Meca-Laguna G., Rocha M., Wagner-Schrittwieser A., Pils V., Meixner B., et al.
2'-O-ribose methylation levels of ribosomal RNA distinguish different types of growth arrest in human dermal fibroblasts.
Journal of Cell Science, 2024. 137(3).

Flores-Villegas M., Rebnegger C., Kowarz V., Prielhofer R., Mattanovich D., and Gasser B.
Systematic sequence engineering enhances the induction strength of the glucose-regulated GTH1 promoter of Komagataella phaffii.
Nucleic Acids Research, 2023. 51(20): 11358–11374.

Hausjell C. S., Klausberger M., Ernst W., and Grabherr R.
Evaluation of an inducible knockout system in insect cells based on co-infection and CRISPR/Cas9.
Plos one, 2023. 18(7): e0289178.

Rebnegger C., Coltman B. L., Kowarz V., Peña D. A., Mentler A., Troyer C., Hann S., Schöny H., Koellensperger G., Mattanovich D., et al.
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Research Square Preprint, 2023.

Seigner J., Zajc C. U., Dötsch S., Eigner C., Laurent E., Busch D. H., Lehner M., and Traxlmayr M. W.
Solving the mystery of the FMC63-CD19 affinity.
Scientific Reports, 2023. 13(1): 23024.

Strobl M. R., Demir H., Stadlmayr G., Stracke F., Hoelzl R., Bohle B., and Wozniak-Knopp G.
Affinity matters for IgE-blocking activity of allergen-specific antibodies.
Allergy, 2023.

Stadlbauer K., Andorfer P., Stadlmayr G., Rüker F., and Wozniak-Knopp G.
Bispecific MAb2 Antibodies Targeting CD59 Enhance the Complement-Dependent Cytotoxicity Mediated by Rituximab.
International Journal of Molecular Sciences, 2022. 23(9): 5208.

Tir N., Heistinger L., Grünwald-Gruber C., Jakob L. A., Dickgiesser S., Rasche N., and Mattanovich D.
From strain engineering to process development: monoclonal antibody production with an unnatural amino acid in Pichia pastoris.
Microbial Cell Factories, 2022. 21(1): 157.

Wagner A., Galicia-Andrés E., Teufl M., Gold L., Obinger C., Sykacek P., Oostenbrink C., and Traxlmayr M. W.
Identification of Activating Mutations in the Transmembrane and Extracellular Domains of EGFR.
Biochemistry, 2022. 61(19): 2049–2062.

Doppler P., Kriechbaum R., Singer B., and Spadiut O.
Make microalgal cultures axenic again-a fast and simple workflow utilizing fluorescence-activated cell sorting.
Journal of Microbiological Methods, 2021. 186: 106256.

Laurent E., Sieber A., Salzer B., Wachernig A., Seigner J., Lehner M., Geyeregger R., Kratzer B., Jager U., Kunert R., et al.
Directed evolution of stabilized monomeric CD19 for monovalent CAR interaction studies and monitoring of CAR-T cell patients.
ACS synthetic biology, 2021. 10(5): 1184–1198.

Mueller A. J., Jung M., Strachan C. R., Herbold C. W., Kirkegaard R. H., Wagner M., and Daims H.
Genomic and kinetic analysis of novel Nitrospinae enriched by cell sorting.
The ISME journal, 2021. 15(3): 732–745.

Tan C. D., Forsthuber A., and Ehmoser E.
Functional proteoliposome-like structure derived from simultaneous evisceration and enucleation of T-lymphoblastoid A3R5. 7 cells: A top-down story.
Experimental Cell Research, 2021. 400(2): 112487.

Vogt S., Bobbili M. R., Stadlmayr G., Stadlbauer K., Kjems J., Rüker F., Grillari J., and Wozniak-Knopp G.
An engineered CD81-based combinatorial library for selecting recombinant binders to cell surface proteins: Laminin binding CD81 enhances cellular uptake of extracellular vesicles.
Journal of Extracellular Vesicles, 2021. 10(11): e12139.

Wagner A., Teufl M., Gold L., Lehner M., Obinger C., Sykacek P., and Traxlmayr M. W.
PhosphoFlowSeq-A High-throughput Kinase Activity Assay for Screening Drug Resistance Mutations in EGFR.
Journal of Molecular Biology, 2021. 433(22): 167210.

Burgard J., Grünwald-Gruber C., Altmann F., Zanghellini J., Valli M., Mattanovich D., and Gasser B.
The secretome of Pichia pastoris in fed-batch cultivations is largely independent of the carbon source but changes quantitatively over cultivation time.
Microbial Biotechnology, 2020. 13(2): 479–494.

Eisenhut P., Mebrahtu A., Moradi Barzadd M., Thalén N., Klanert G., Weinguny M., Sandegren A., Su C., Hatton D., Borth N., et al.
Systematic use of synthetic 5'-UTR RNA structures to tune protein translation improves yield and quality of complex proteins in mammalian cell factories.
Nucleic Acids Research, 2020. 48(20): e119–e119.

Heistinger L., Gasser B., and Mattanovich D.
Komagataella phaffii YPS1-5 encodes the alpha-factor degrading protease Bar1.
FEMS Yeast Research, 2020. 20(3): foaa024.

Herzog P., Borghi E., Traxlmayr M., Obinger C., Sikes H., and Peterbauer C.
Developing a cell-bound detection system for the screening of oxidase activity using the fluorescent peroxide sensor roGFP2-Orp1.
Protein Engineering, Design and Selection, 2020. 33: gzaa019.

Schwaigerlehner L., Mayrhofer P., Diem M., Steinfellner W., Fenech E., Oostenbrink C., and Kunert R.
Germinality does not necessarily define mAb expression and thermal stability.
Applied microbiology and biotechnology, 2019. 103: 7505–7518.

Vogt S., Stadlmayr G., Stadlbauer K., Sádio F., Andorfer P., Grillari J., Rüker F., and Wozniak-Knopp G.
Stabilization of the CD81 large extracellular loop with de novo disulfide bonds improves its amenability for peptide grafting.
Pharmaceutics, 2018. 10(3): 138.

Vogt S., Bobbili M. R., Stadlmayr G., Stadlbauer K., Kjems J., Rüker F., Grillari J., and Wozniak-Knopp G.
An engineered CD81-based combinatorial library for selecting recombinant binders to cell surface proteins: Laminin binding CD81 enhances cellular uptake of extracellular vesicles.
Journal of Extracellular Vesicles, 2021. 10(11): e12139.

Terlecki-Zaniewicz L., Pils V., Bobbili M. R., Lämmermann I., Perrotta I., Grillenberger T., Schwestka J., Weiß K., Pum D., Arcalis E., et al.
Extracellular vesicles in human skin: cross-talk from senescent fibroblasts to keratinocytes by miRNAs.
Journal of Investigative Dermatology, 2019. 139(12): 2425–2436.

Terlecki-Zaniewicz L., Lämmermann I., Latreille J., Bobbili M. R., Pils V., Schosserer M., Weinmüllner R., Dellago H., Skalicky S., Pum D., et al.
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