For various screening methods BMoSA utilizes a continuous process using several pieces of equipment to combine microorganisms and to analyze their bioactive molecular metabolites. Each section of the method is adaptable and accessible to external users. The method comprises 1) assembly of assays in MTPs with inoculation and incubation, 2) amplification of plates and 3) continuous growth assays. For example, microbial cultures growing in liquid or on solid are added to media with variable ingredients (e.g. antifungals, antibiotics, stress factors, small molecules). We have developed methods to quantify growth of filamentous fungi (Canovas et al.), Arabidopsis and other target organisms.
Incubation can be combined with spectroscopy using an automatic incubator and robotic arm in sync with a multiwell Spectrometer. Microbial growth is measured in parallel with 42 plates. Possible readouts include absorbance, fluorescence, and luminescence.
Zangl I, Beyer R, Pap IJ, Strauss J, Aspöck C, Willinger B, Schüller C. Human Pathogenic Candida Species Respond Distinctively to Lactic Acid Stress. J Fungi (Basel). 2020 Dec 8;6(4):348. doi: 10.3390/jof6040348. PMID: 33302409; PMCID: PMC7762603.
Beyer R, Zangl I, Seidl B, Pap I-J, Lackner M, Strauss J, Willinger B, Schüller C. Distinct properties of human pathogenic Candida species revealed by systematic comparative phenotypic screening of clinical isolates.
mSystems. 2026 Jan 20;11(1):e0078625. doi: 10.1128/msystems.00786-25. Epub 2025 Dec 8. PMID: 41358755; PMCID: PMC12817934.
