About the instrument

To analyze life with as little disturebnace as possible, one need excellent imaging performance combined with low phototoxicity and high speed.

LSM980 from ZEISS is an optimized confocal system for 4D imaging, spectral multiplexing by simultaneous spectral detection of multiple labels, and LSM Plus modus enables you to gather more information while increasing productivity. 

The AiryScan2 modul makes gentle super-resolution imaging possible (x,y: ~100 nm). It captures larger fields of view or dynamic processes with the flexible Multiplex modes using shorter acquisition times than previously possible. Moreover, it can push resolution further to identify new structures with AiryScan Joint Deconvolution (jDCV).

With ZEN Connect you can document and share all details of your experiments. You'll always maintain context as you combine overview images, ROIs and additional data, even across imaging modalities provided by the manufacturer.

Technical details

  • Microscope stand: Axio Imager.Z2, an upright configuration
  • Scanning head: RGB f/LSM 980
  • Stage: motorized, inserts for slides, Petri dishes
  • Hardware auto-focus control
  • Detectors for confocal and "super-reoslution": T-PMT, 2 PMTs + 32 channel GaAsP o.Count., AiryScan2 detector
  • Detector for wide-field: AxioCam 820 mono
  • Lightsources for confocal and "super-resolution":
    • 405 nm (cw, diode laser)
    • 445 nm (cw, diode laser)
    • 488 nm (cw, diode laser)
    • 514 nm (cw, diode laser)
    • 561 nm (cw, diode laser)
    • 639 nm (cw, diode laser)
  • Lightsource for wide-field: Colibri-7 LED, type R(G/Y)CBV-UV

Objectives (default set):

  • EC Plan-NEOFUAR 2,5x/0,085
  • PApo 10x/0,45
  • LD LCI PApo 25x/0,8 Imm Korr D
  • LD LCI PApo 40x/1,2 Korr FCS
  • W PApo 40x/1,0 DIC Vis-IR
  • LCI PN 63x/1,3 Imm Korr DIC
  • C PApo 63x/1,4 Oil DIC UV-IR (otional)

 

ZEN 3.8 DLic Software for image acquisition, modules/toolkits:

  • Advanced acquisition + Smart acquisition, 2D, 3D
  • Sample Navigator + ZEN connect
  • Molecular quantification
  • LSM Plus
  • AiryScan 2 with Mplx plus
  • AiryScan Joint Deconvolution