The Schäffer Research Group uses the following equipment and infrastructure:

Microbiology

  • Autoclaves: ThermoScientific Varioklav, Certoklav CV-EL 18GS
  • Balances: Mettler PJ400, Sartorius QUINTIX65-1S,
  • Branson Ultrasonics™ Sonifier™ SFX150 Desintegrator
  • Centrifuges: Beckman Coulter Avanti J-76 XP
  • Table top centrifuges: Eppendorf Centrifuges MiniSpin; Eppendorf Centrifuge 5804 R; Eppendorf Centrifuge 5424 R
  • Dewar
  • Freezer: ThermoFisher Scientific™ HERAfreeze™ HFU T Series -86 °C
  • Incubators: IGS60, Heratherm; Orbital incubators: New Brunswick Innova® 44/44R
  • Lab ice making machine
  • Microwave
  • Milli-Q® IX Water Purification System
  • Ultracentrifuges: Beckman Coulter, Optima™ XPN-100; Beckman-Coulter Avanti J-26 XP
  • Ultrospec 10
  • Water baths: ThermoFisher Scientific™

 

Biosafety level 2 laboratory

  • AnaeroJars: Oxoid
  • Certoklav CV-EL 18GS
  • CO2-Incubator: MMM CO2 Cell comfort line
  • Glove box - Anaerobic Workstation DG250
  • Incubators: IGS60, Heratherm
  • Laminars: Thermo Scientific KS12 Safety work bench KL.II
  • Magnetic stirrers with hot plate: Sigma-Aldrich
  • Microcentrifuge Pico 17
  • Swing bucket centrifuge: Megafuge 16 R, ThermoFisher Scientific

 

Molecular biology and genetics

  • Hybridization oven: Hybaid Shake ‘n' Stack, Thermo Scientific 6242
  • RT-PCR: MJ Mini Opticon, BioRad
  • PCR machines: iCycler, BioRad
  • DNA Electrophoresis Cells: Mini-Sub Cell GT Horizontal Electrophoresis, BioRad
  • Electroporator: Gene Pulser Xcell Microbial System

 

Biochemistry

  • Amino acid analyzer
  • Bio-Rad Gel Doc XR+ UV Gel Documentation Imaging System + Quantity One Basic
  • Christ lyophilisator Labor Alpha 3-4 LSCbasic
  • Chromatography: ÄKTA, FPLC; HPLC: Thermo Fisher Scientific UltiMate 3000¸ Wyatt MALS Dawn8+
  • Circular dichroism: ChiraScan Applied Photophysics; temperature-controlled; CD, UV-VIS & fluorescence detection
  • Dionex ICS 3000
  • Dynamic Light Scattering and Electrophoretic (zeta potential) measurements: Malvern Zetasizer
  • Electrophoresis and blot: Mini Protean, trans-blot module, BioRad
  • Fluorimeter: Perkin Elmer FL 6500
  • Infrared Imager: LICOR Odyssey
  • Magnetic stirrer: Isotemp
  • Microplate reader: Infinite F200 Tecan
  • Nanodrop
  • pH-meter: Mettler Seven Compact S210 Basic
  • Photometer: Eppendorf BioSpectrometer basic; Applied Photophysics Chirascan CD Spectrometer; Hitachi Model F-7000 flourescence spectrophotometer; Hitachi Model U-3900 UV-VIS spectrophotometer
  • Plate shaker: Orbital shaker, IKA
  • Power supplies: BioRad
  • Thermomixer: Eppendorf Thermomixer comfort
  • Ultrasonic bath
  • Vortex

 

Cell culture

  • CO2-Incubator: MMM CO2 Cell comfort line
  • FACS: BD FACSCanto II Flow Cytometer

 

Microscopy

  • Cryo-Preparation: High Pressure Freezing - Leica EM HPM 100 (Leica Microsystems, Austria); Freeze Fracture, Etching and e-beam Coating - Leica EM ACE900 (Leica Microsystems, Austria)
  • Cryo-Scanning Electron Microscope - Apreo VS (Thermo Scientific, The Netherlands)
  • Fluorescence microscope: Nikon TE-2000-S
  • Light microscope: Polyvar Reichert-Jung
  • Microtome: Leica
  • Reflectance, transmission and polarized light microscopy - Nikon ME600
  • Thin metal film coating: Leica- BalTec
  • Transmission Electron Microscope - FEI TECNAI G2 (FEI Europe B. V, Austria)

 

BOKU Core Facilities https://boku.ac.at/cf

  • Core Facility Biomolecular & Cellular Analysis
  • Core Facility Multiscale Imaging
  • Core Facility Mass Spectrometry
  • Core Facility Bioinformatics