DAGZ > Topics for theses - Abschlussarbeitenbörse
Regulation of plant development by O-glycosylation
O-glycosylation of nuclear and cytosolic proteins is an essential post-translational modification that regulates signaling pathways in the course of plant development. In contrast to other glycosylation events, a single monosaccharide - N-acetylglucosamine (GlcNAc) or fucose - is attached to serine or threonine residues of a high number of very diverse proteins. We are studying the effect of this type of protein glycosylation on different aspects of plant development such as flowering time, shoot branching and leaf size.
We are looking for a Master student with background in molecular biology/biochemistry and an interest in plant biology, to study the interaction between two different O-glycosyltransferases and a family of transcription factors. Techniques include yeast-two hybrid assays and co-immunoprecipitation, as well as analysis of reporter-lines to study the effect of O-glycosylation on the function of transcription factors.
Duration: 6 months, starting in September 2022.
Payment: 'geringfügig' (ca. 470 € / month)
The work will take place at DAGZ in Muthgasse 18.
Contact:
Doris Lucyshyn e-mail
Identification and characterization of lectins important for plant development
O-glycosylation of nuclear and cytosolic proteins is an essential post-translational modification that regulates signaling pathways in the course of plant development. In contrast to other glycosylation events, a single monosaccharide - N-acetylglucosamine (GlcNAc) or fucose - is attached to serine or threonine residues of a high number of very diverse proteins. We are studying the effect of this type of protein glycosylation on different aspects of plant development, and how it is regulated by interaction with lectins.
We are looking for a Master student with background in biochemistry / molecular biology, and an interest in plant biology, to study the interaction between cytosolic lectins and O-glycosylated proteins. Techniques include co-immunoprecipitation and binding assays, as well as design and cloning of constructs to generate plant lines lacking a number of specific plant lectins, using the CRISPR-Cas9-system.
Duration: 6 months, starting in September 2022.
Payment: 'geringfügig' (ca. 470 € / month).
The work will take place at DAGZ in Muthgasse 18.
Ansprechperson:
Doris Lucyshyn e-mail