2024
Herdina, A.N.; Bozdogan, A.; Aspermair, P.; Dostalek, J.; Klausberger, M.; Lingg, N.; Cserjan-Puschmann, M.; Pereira Aguilar, P.; Auer, S.; Demirtas, H.; Andersson, J.; Lötsch, F.; Holzer, B.; Steinrigl, A.; Thalhammer, F.; Schellnegger, J.; Breuer, M.; Knoll, W.; Strassl, R. (2024)
Bridging basic science and applied diagnostics: Comprehensive viral diagnostics enabled by graphene-based electronic biosensor technology advancements.
Biosens Bioelectron, 267:116807
Guzman Ruiz, L.; Zollner, A.M.; Hoxie, I.; Küchler, J.; Hausjell, C.; Mesurado, T.; Krammer, F.; Jungbauer, A.; Pereira Aguilar, P.; Klausberger, M.; Grabherr, R. (2024b)
Enhancing NA immunogenicity through novel VLP designs.
Vaccine, 42:126270
Zollner, A.; Guzman Ruiz, L.; Mayer, V.; Stohl, S.; Jakob, L.A.; Lingg, N.; Klausberger, M.; Jungbauer, A.; Pereira Aguilar, P. (2024)
Heparin-affinity chromatography is a generic purification platform for chimeric gag VLPs displaying different viral surface antigens.
Sep Purif Technol, 340:126673
Guzman Ruiz, L.; Zollner, A.M.; Hoxie, I.; Arcalis, E.; Krammer, F.; Klausberger, M.; Jungbauer, A.; Grabherr, R. (2024a)
Increased efficacy of influenza virus vaccine candidate through display of recombinant neuraminidase on virus like particles.
Front Immunol, 15:1425842
Zemkollari, M.; Oostenbrink, C.; Grabherr, R.; Staudacher, E. (2024)
Molecular cloning, characterisation and molecular modelling of two novel T-synthases from mollusc origin.
Glycobiology, 34(4)
Toth, M.; Reithofer, M.; Dutra, G.; Pereira Aguilar, P.; Dürauer, A.; Grabherr, R. (2024)
Comprehensive Comparison of Baculoviral and Plasmid Gene Delivery in Mammalian Cells.
Viruses, 16:426
Achleitner, L.; Winter, M.; Pereira Aguilar, P.; Lingg, N.; Jungbauer, A.; Klausberger, M.; Satzer, P. (2024)
Robust and resource-efficient production process suitable for large-scale production of baculovirus through high cell density seed train and optimized infection strategy.
N Biotechnol, 80:46–55
Berguido, F, J.; Kangethe, R. T.; Shell, W.; Wijewardana, V.; Grabherr, R.; Catoli, G.; Lamien C. E. (2024)
Different Neutralizing Antibody Responses of Heterologous Sera on Sheeppox and Lumpy Skin Disease Viruses.
Viruses, 16(7):1127
Ziu, T.; Sambur, E.; Ruzsics, Z.; Hengel, H.; Grabherr, R.; Höfinger, S.; Harant, H. (2024)
In vitro profiling of the antiviral peptide TAT-I24.
IJMS, 25(19):10463
Thoma, J.; Grabherr, R.; Staudacher, E. (2024)
Expression and Characterization of a β-Galactosidase from the Pacific Oyster, Crassostrea gigas, and Evaluation of Strategies for Testing Substrate Specificity.
Int. J. Mol. Sci.,24(20): 15287
van Nieuwenhoven, R.W.; Bürger, A.M.; Mears, L.L.E.; Gebeshuber, I.C. (2024)
Verifying antibacterial properties of nanopillars on cicada wings.
Appl Nanosci, 14:531–541
2023
Reithofer, M.; Boell, S. M.; Kitzmueller, C.; Horak, F.; Bohle, B.; Jahn-Schmid, B. (2023)
T-cell subset changes during the first year of pre-seasonal allergoid allergen-specific immunotherapy.
Heliyon, 9(11): e21878, ISSN 2405-8440
Berguido, F. J.; Chibssa, T. R.; Loitsch, A.; Liu, Y.; Krstevski, K.; Djadjovski, I.; Tuppurainen, E.; Petrovic, T.; Vidanovic, D.; Caufour, P.; Settypalli, T. B. K.; Grünwald-Gruber, C.; Grabherr, R.; Diallo, A.; Cattoli, G.; Lamien, C. E. (2023)
Harnessing Attenuation-Related Mutations of Viral Genomes: Development of a Serological Assay to Differentiate between Capripoxvirus-Infected and -Vaccinated Animals.
Viruses, 15(12): 2318
Hausjell, C. S.; Ernst, W.; Grünwald-Gruber, C.; Arcalis, E.; Grabherr, R. (2023)
Quantitative proteomic analysis of extracellular vesicles in response to baculovirus infection of a Trichoplusia ni cell line.
PLoS ONE, 18(1): e0281060
2022
Zhu, R.; Canena, D.; Sikora, M.; Klausberger, M.; Seferovic, H.; Mehdipour, A. R.; Hain, L.; Laurent, E.; Monteil, V.; Wirnsberger, G.; Wieneke, R.; Tampé, R.; Kienzl, N. F.; Mach, L.; Mirazimi, A.; Oh, Y. J.; Penninger, J. M.; Hummer, G.; Hinterdorfer, P. (2022)
Force-tuned avidity of spike variant-ACE2 interactions viewed on the single-molecule level.
Nature Communications, 13(1), 7926
Chan, J. F. W.; Oh, Y. J.; Yuan, S.; Chu, H.; Yeung, M. L.; Canena, D.; Chan, C. S.; Poon, K. M.; Chan, C. Y. C.; Zhang, J.; Cai, J.; Ye, Z.; Wen, L.; Yuen, T. T.; Chik, K. K. H.; Shuai, H. V.; Wang, Y.; Hou, Y.; Luo, C.; Chan, W. M.; Qin, Z.; Sit, K. Y.; Au, T. W. K.; Legendre, M.; Zhu, R.; Hain, L.; Seferovic, H.; Tampé, R.; To, K. K. W.; Chan, K. H.; Thomas, D. G.; Klausberger, M.; Xu, C.; Moon, J. J.; Stadlmann, J.; Penninger, J. M.; Oostenbrink, C.; Hinterdorfer, P.; Yuen, K. Y.; Markovitz, D. M. (2022)
A molecularly engineered, broad-spectrum anti-coronavirus lectin inhibits SARS-CoV-2 and MERS-CoV infection in vivo.
Cell Reports Medicine, 3(10), 100774
Thoma, J.; Stenitzer, D.; Grabherr, R.; Staudacher, E. (2022)
Identification, Characterization, and Expression of a β-Galactosidase from Arion Species (Mollusca).
Biomolecules, 12(11), 1578
Berguido, F. J.; Gelaye, E.; Liu, Y.; Davaasuren, B.; Krstevski, K.; Djadjovski, I.; Ivanova, E.; Goujgoulova, G.; Loitsch, A.; Tuppurainen, E.; Chibssa, T. R.; Caufour, P.; Samojlović, M.; Lazić, S.; Petrović, T.; Vidanović, D.; Bertagnoli, S.; Grabherr, R.; Diallo, A.; Cattoli, G.; Lamien, C. E. (2022)
Development and Optimization of Indirect ELISAs for the Detection of Anti-Capripoxvirus Antibodies in Cattle, Sheep, and Goat Sera.
Microorganisms, 10(10), 1956
Klanschnig, M.; Cserjan-Puschmann, M.; Striedner, G.; Grabherr, R. (2022)
CRISPRactivation-SMS, a message for PAM sequence independent gene up-regulation in Escherichia coli.
Nucleic Acids Research, 50(18), 10772-10784
Ly, D.; Mayrhofer, S.; Domig, K. J. (2022)
Important initial step in developing potential Cambodian starters for fermented foods: a detailed assessment of phenotypic antibiotic susceptibility data.
Frontiers in Food Science and Technology, 14.
Atzl, M.; Muendlein, A.; Winder, T.; Fraunberger, P.; Brandtner, E. M.; Geiger, K.; Klauberger, M.; Duerkop, M.; Sprenger, L.; Mutschlechner, B.; Volgger, A.; Benda, M.; Severgnini, L.; Jaeger, J. B.;Drexel, H.; Lang, A.; Leiherer, A. (2022)
SARS-CoV-2 RBD-specific and NP-specific antibody response of healthcare workers in the westernmost Austrian state Vorarlberg: A prospective cohort study.
BMJ open, 12(5), e052130
Puente‐Massaguer, E.; González‐Domínguez, I.; Strobl, F.; Grabherr, R.; Striedner, G.; Lecina, M.; Gòdia, F. (2022)
Bioprocess characterization of virus‐like particle production with the insect cell baculovirus expression system at nanoparticle level.
Journal of Chemical Technology & Biotechnology, 97(9), 2456-2465
Klausberger, M.; Kienzl, N. F.; Stadlmayr, G.; Grünwald-Gruber, C.; Laurent, E.; Stadlbauer, K.; Stracke, F.; Vierlinger, K.; Hofner, M.; Manhart, G.; Gerner, W.; Grebien, F.; Weinhäusel, A.; Mach, L.; Wozniak-Knopp, G. (2022)
Designed SARS-CoV-2 receptor binding domain variants form stable monomers.
Biotechnol J.,17(5):e2100422
2021
Castellani, C.; Obermuller, B.; Kienesberger, B.; Singer, G.; Peterbauer, C.; Grabherr, R.; Mayrhofer, S.; Klymiuk, I.; Horvath, A.; Stadlbauer, V.; Russmayer, H.; Miekisch, W.; Fuchs, P.; Till, H.; Heinl, S. (2021)
Production, Storage Stability, and Susceptibility Testing of Reuterin and Its Impact on the Murine Fecal Microbiome and Volatile Organic Compound Profile.
Front Microbiol.; 699858
Chibssa, T. R.; Kangethe, R. T.; Berguido, F. J.; Settypalli, TBK.; Liu, Y.; Grabherr, R.; Loitsch, A.; Sassu, E. L.; Pichler, R.; Cattoli, G.; Diallo, A.; Wijewardana, V.; Lamien, C. E. (2021)
Innate Immune Responses to Wildtype and Attenuated Sheeppox Virus Mediated Through RIG-1 Sensing in PBMC In-Vitro.
Front Immunol.; 12, 666543
Chibssa, T. R.; Liu, Y.; Sombo, M.; Lichoti, J. K.; Erdenebaatar, J.; Boldbaatar, B.; Grabherr, R.; Settypalli, T. B. K.; Berguido, F. J.; Loitsch, A.; Damena, D.; Cattoli, G.; Diallo, A.; Lamien, C. E. (2021)
Use of an Alignment-Free Method for the Geographical Discrimination of GTPVs Based on the GPCR Sequences.
Microorganisms.; 9(4), 855
Chibssa, T. R.; Sombo, M.; Lichoti, J. K.; Adam, T. I. B.; Liu, Y.; Abd Elraouf, Y.; Grabherr, R.; Settypalli, T. B. K.; Berguido, F. J.; Loitsch, A.; Sahle, M.; Cattoli, G.; Diallo, A.; Lamien, C. E. (2021)
Molecular Analysis of East African Lumpy Skin Disease Viruses Reveals a Mixed Isolate with Features of Both Vaccine and Field Isolates.
Microorganisms.; 9(6), 1142
Kienesberger, B.; Obermuller, B.; Singer, G.; Mittl, B.; Grabherr, R.; Mayrhofer, S.; Heinl, S.; Stadlbauer, V.; Horvath, A.; Miekisch, W.; Fuchs, P.; Klymiuk, I.; Till, H.; Castellani, C. (2021)
(S)-Reutericyclin: Susceptibility Testing and In Vivo Effect on Murine Fecal Microbiome and Volatile Organic Compounds.
Int J Mol Sci.2021.; 22(12), 6424
Puente-Massaguer, E.; Grau-Garcia, P.; Strobl, F.; Grabherr, R.; Striedner, G.; Lecina, M.; Godia, F. (2021)
Stable Sf9 cell pools as a system for rapid HIV-1 virus-like particle production.
J Chem Technol Biot. 2021.; 96(12): 3388-3397
Puente-Massaguer, E.; Grau-Garcia, P.; Strobl, F.; Grabherr, R.; Striedner, G.; Lecina, M.; Gòdia, F. (2021)
Accelerating HIV-1 VLP production using stable High Five insect cell pools.
Biotechnol J.; 16(4):e2000391
Schuller, A.; Cserjan-Puschmann, M.; Köppl, C.; Grabherr, R.; Wagenknecht, M.; Schiavinato, M.; Dohm, J. C.; Himmelbauer, H.; Striedner, G.; (2021)
Adaptive Evolution in Producing Microtiter Cultivations Generates Genetically Stable Escherichia coli Production Hosts for Continuous Bioprocessing.
Biotechnol J.; 16(3):e2000376
De Vos, J.; Pereira Aguilar, P.; Köppl, C.; Fischer, A.; Grünwald-Gruber, C.; Dürkop, M.; Klausberger, M.; Mairhofer, J.; Striedner, G.; Cserjan-Puschmann, M.; Jungbauer, A.; Lingg, N. (2021)
Production of full-length SARS-CoV-2 nucleocapsid protein from Escherichia coli optimized by native hydrophobic interaction chromatography hyphenated to multi-angle light scattering detection.
Talanta 235:122691
Hoffmann, D.; Mereiter, S.; Jin Oh, Y.; Monteil, V.; Elder, E.; Zhu, R.; Canena, D.; Hain, L.; Laurent, E.; Grünwald‐Gruber, C.; Klausberger, M. (2021)
Identification of lectin receptors for conserved SARS‐CoV‐2 glycosylation sites.
The EMBO Journal.;40(19):e108375
Perkmann, T.; Koller, T.; Perkmann-Nagele, N.; Klausberger, M.; Duerkop, M.; Holzer, B.; Hartmann, B.; Mucher, P.; Radakovics, A.; Ozsvar-Kozma, M.; Wagner, O. F.; Binder, C. J.; Haslacher, H. (2021)
Spike protein antibodies mediate the apparent correlation between SARS-CoV-2 nucleocapsid antibodies and neutralization test results.
Microbiol Spectr 9:e00218-21
Schwestka, J.; König-Beihammer, J.; Shin, Y.-J.; Vavra, U.; Kienzl, N. F.; Grünwald-Gruber, C.; Maresch, D.; Klausberger, M.; Laurent, E.; Stadler, M.; Manhart, G.; Huber, J.; Hofner, M.; Vierlinger, K.; Weinhäusel, A.; Swoboda, I.; Binder, C. J.; Gerner, W.; Grebien, F.; Altmann, F.; Mach, L.; Stöger, E.; Strasser, R. (2021)
Impact of Specific N-Glycan Modifications on the Use of Plant-Produced SARS-CoV-2 Antigens in Serological Assays.
Front. Plant Sci. 12:747500
Shin, Y.-J.; König-Beihammer, J.; Vavra, U.; Schwestka, J.; Kienzl, N. F.; Klausberger, M.; Laurent, E.; Grünwald-Gruber, C.; Vierlinger, K.; Hofner, M.; Margolin, E.; Weinhäusel, A.; Stöger, E.; Mach, L.; Strasser, R (2021)
N-Glycosylation of the SARS-CoV-2 Receptor Binding Domain Is Important for Functional Expression in Plants.
Front. Plant Sci. 12:689104
Klausberger, M.; Duerkop, M.; Haslacher, H.; Wozniak-Knopp, G.; Cserjan-Puschmann, M.; Perkmann, T.; Lingg, N.; Aguilar, P. P.; Laurent, E.; De Vos, J.; Hofner, M.; Holzer, B.; Stadler, M.; Manhart, G.; Vierlinger, K.; Egger, M.; Milchram, L.; Gludovacz, E.; Marx, N.; Köppl, C.; Tauer, C.; Beck, J.; Maresch, D.; Grünwald-Gruber, C.; Strobl, F.; Satzer, P.; Stadlmayr, G.; Vavra, U.; Huber, J.; Wahrmann, M.; Eskandary, F.; Breyer, M. K.; Sieghart, D.; Quehenberger, P.; Leitner, G.; Strassl, R.; Egger, A. E.; Irsara, C.; Griesmacher, A.; Hoermann, G.; Weiss, G.; Bellmann-Weiler, R.; Loeffler-Ragg, J.; Borth, N.; Strasser, R.; Jungbauer, A.; Hahn, R.; Mairhofer, J.; Hartmann, B.; Binder, N. B.; Striedner, G.; Mach, L.; Weinhäusel, A.; Dieplinger, B.; Grebien, F.; Gerner, W.; Binder, C. J.; Grabherr, R. (2021)
A Comprehensive Antigen Production and Characterisation Study for Easy-to-Implement, Specific and Quantitative SARS-CoV-2 Serotests.
EBioMedicine 67: 103348
2020
Ly, D.; Mayrhofer, S.; Schmidt, J.; Zitz, U.; Domig, K. J. (2020)
Biogenic Amine Contents and Microbial Characteristics of Cambodian Fermented Foods.
Foods, 9(2): 198
Cicconardi, F.; Krapf, P.; D'Annessa, I.; Gamisch, A.; Wagner, H. C.; Nguyen, A. D.; Economo, E. P.; Mikheyev, A. S.; Guenard, B.; Grabherr, R.; Andesner, P.; Wolfgang, A.; Di Marino, D.; Steiner, F.M.; Schlick-Steiner, B.C. (2020)
Genomic Signature of Shifts in Selection in a Subalpine Ant and Its Physiological Adaptations.
Mol Biol Evol.; 37(8): 2211-2227.
Egger, E.; Tauer, C.; Cserjan-Puschmann, M.; Grabherr, R.; Striedner, G. (2020)
Fast and antibiotic free genome integration into Escherichia coli chromosome.
Sci Rep-UK.; 10(1), 16510
Obermuller, B.; Singer, G.; Kienesberger, B.; Klymiuk, I.; Sperl, D.; Stadlbauer, V.; Horvath, A.; Miekisch, W.; Gierschner, P.; Grabherr, R.; Gruber, H. J.; Semeraro, M. D.; Till, H.; Castellani, C. (2020)
The Effects of Prebiotic Supplementation with OMNi-LOGiC(R)FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia.
Nutrients.; 12(7), 2029
Puente-Massaguer, E.; Strobl, F.; Grabherr, R.; Striedner, G.; Lecina, M.; Godia, F. (2020)
PEI-Mediated Transient Transfection of High Five Cells at Bioreactor Scale for HIV-1 VLP Production.
Nanomaterials-Basel.; 10(8), 1580
Ruzsics, Z.; Hoffmann, K.; Riedl, A.; Krawczyk, A.; Widera, M.; Sertznig, H.; Schipper, L.; Kapper-Falcone, V.; Debreczeny, M.; Ernst, W.; Grabherr, R.; Hengel, H.; Harant, H. (2020)
A Novel, Broad-Acting Peptide Inhibitor of Double-Stranded DNA Virus Gene Expression and Replication.
Front Microbiol.; 11, 601555
Schuller, A.; Cserjan-Puschmann, M.; Tauer, C.; Jarmer, J.; Wagenknecht, M.; Reinisch, D.; Grabherr, R.; Striedner, G. (2020)
Escherichia coli σ70 promoters allow expression rate control at the cellular level in genome-integrated expression systems.
Microbial Cell Factories, 19 (1):58
Klausberger, M.; Leneva, I. A.; Egorov, A.; Strobl, F.; Ghorbanpour, S. M.; Falynskova, I. N.; Poddubikov, A. V.; Makhmudova, N. R.; Krokhin, A.; Svitich, O. A.; Grabherr, R. (2020)
Off-target effects of an insect cell-expressed influenza HA-pseudotyped Gag-VLP preparation in limiting postinfluenza Staphylococcus aureus infections.
Vaccines, 38(4):859-867
Tscheppe, A.; Palmberger, D.; van Rijt, L.; Kalic, T.; Mayr, V.; Palladino, C.; Hemmer, W.; Hafner, C.; Bublin, M.; van Ree, R.; Grabherr, R.; Radauer, C.; Breiteneder, H. (2020)
Development of a novel Ara h 2 hypoallergen with no IgE binding or anaphylactogenic activity.
J. Allergy and Clinical Immunology, 145(1):229-238
2019
Roth, N.; Käsbohrer, A.; Mayrhofer, S.; Zitz, U.; Hofacre, C.; Domig, K.J. (2019)
The application of antibiotics in broiler production and the resulting antibiotic resistance in Escherichia coli: A global overview.
Poult Sci., 98(4):1791-1804
Ly, D.; Mayrhofer, S.; Yogeswara, A. B. I.; Nguyen, T.; Domig, K. J.(2019)
Identification, Classification and Screening for γ-Amino-butyric Acid Production in Lactic Acid Bacteria from Cambodian Fermented Foods.
Biomolecules, 9(12):768
Kos, I.; Maksimović, A. Z.; Zunabović-Pichler, M.; Mayrhofer, S.; Domig K. J.; Mrkonjić Fuka, M. (2019)
The Influence of Meat Batter Composition and Sausage Diameter on Microbiota and Sensory Traits of Artisanal Wild Boar Meat Sausages.
Food Technol Biotechnol., 57(3): 378–387
Fink, M.; Vazulka, S.; Egger, E.; Jarmer, J.; Grabherr, R.; Cserjan-Puschmann, M.; Striedner, G. (2019)
Microbioreactor Cultivations of Fab-Producing Escherichia coli Reveal Genome-Integrated Systems as Suitable for Prospective Studies on Direct Fab Expression Effects.
Biotechnol J. 2019.; 14(11):e1800637
Klausberger M.; Leneva I. A.; Falynskova, I. N.; Vasiliev, K.; Poddubikov, A. V.; Lindner, C.; Kartaschova, N. P.; Svitich, O. A.; Stukova, M.; Grabherr, R.; Egorov, A. (2019)
The potential of influenza HA-specific immunity in mitigating lethality of postinfluenza pneumococcal infections.
Vaccines, 7(4):187
Fink, M.; Vazulka, S.; Egger, E.; Jarmer, J.; Grabherr, R.; Cserjan-Puschmann, M.; Striedner, R. (2019)
Micro-bioreactor cultivations of Fab producing Escherichia coli reveal genome-integrated systems as suitable for prospective studies on direct Fab expression effects.
Biotechnology Journal, 14(11):e1800637
Chibssa, T. R.; Settypalli, B.; Berguido, F. J.; Grabherr, R.; Loitsch, A.; Tuppurainen, E.; Nick, N.; Tounkura, K.; Madani, H.; Omani, A-. Diop, M.; Cattoli, G.; Diallo A.; Lamien, C. E. (2019)
An HRM Assay to Differentiate Sheeppox Virus Vaccine Strains from Sheeppox Virus Field Isolates and other Capripoxvirus Species.
Scientific Reports, 9(1):6646
Nika, L.; Cuadra-Castano, S.; Arunkumar, A. G.; Grünwald-Gruber, C.; McMahon, M.; Koczka, K.; Garcia-Sastre, A.; Krammer, F.; Grabherr, R. (2019)
A HER2-displaying virus-like particle vaccine protects from challenge with mammary carcinoma cells in a mouse model.
MDPI Vaccines, 7(2):41
Koczka K.; Ernst W.; Palmberger D.; Klausberger M.; Nika L.; Grabherr R. (2019)
Development of a Dual-Vector System Utilizing MicroRNA Mimics of the Autographa californica miR-1 for an Inducible Knockdown in Insect Cells.
Int. J. Mol. Sci. 20(3):533
Nguyen, H.-M.; Pham, M.-L.; Stelzer, E.; Plattner, E.; Grabherr, R.; Mathiesen, G.; Peterbauer, C.; Haltrich, D.; Nguyen, T.-H. (2019)
Constitutive expression and cell-surface display of a bacterial β-mannanase in Lactobacillus plantarum.
Microbial Cell Factories, 18(76)
2018
Chibssa,T. R.; Grabherr, R.; Loitsch, A.; Settypalli, T. B. K.; Tuppurainen, E.; Nwankpa, N.; Tounkara, K.; Madani, H.; Omani.; A.; Diop, M.; Cattoli, G.; Diallo, A.; Lamien, C. E. (2018)
A gel-based PCR method to differentiate sheeppox virus field isolates from vaccine strains.
Virol J., 15(1):59
Koczka, K.; Peters, P.; Ernst, W.; Himmelbauer, H.; Nika, L.; Grabherr, R. (2018)
Comparative transcriptome analysis of a Trichoplusia ni cell line reveals distinct host responses to intracellular and secreted protein products expressed by recombinant baculoviruses.
J. Biotechnology, 270:61–69
2017
Nika, L.; Wallner, J.; Palmberger, D.; Koczka, K.; Vorauer-Uhl, K.; Grabherr R. (2017)
Expression of full-length HER2 protein in Sf9 insect cells and its presentation on the surface of budded virus-like particles.
Protein Expression and Purification, 136:27-38.
Sauer, M.; Russmayer, H.; Grabherr, R.; Peterbauer, C. K.; Marx. H. (2017)
The efficient clade - lactic acid bacteria for industrial chemical production.
Trends Biotechnol.; 28(8):756-769
Gelaye, E.; Mach, L.; Kolodziejek, J.; Grabherr, R.; Loitsch, A.; Nowotny, N.; Diallo, A.; Lamien, C. E. (2017)
A novel HRM assay for the simultaneous detection and differentiation of eight poxviruses of medical and veterinary importance.
Scientific Reports, 7:42892
Heinl, S.; Grabherr R. (2017)
Systems biology of robustness and flexibility: Lactobacillus buchneri - a show case.
J. Biotechnology 257:61-69
2016
Mairhofer, J.; Stargardt, P.; Feuchtenhofer, L.; Pontiller, J.; Cserjan-Puschmann, M.; Grabherr, R.; Striedner, G. (2016)
Innovation without growth: Non-growth associated recombinant protein production in Escherichia coli.
New Biotechnology, 33:S26
Gelaye, E.; Achenbach, J. A.; Ayalet, G.; Yami, M.; Grabherr, R.; Loitsch, A.; Diallo, A.; Lamien, C. E. (2016)
Genetic characterization of poxviruses in Camelus dromedarius in Ethiopia, 2011–2014.
Antiviral Research, 134:17-25
Heiss, S.; Hörmann, A.; Tauer, C.; Sonnleitner, M.; Egger, E.; Grabherr, R.; Heinl, S. (2016)
Evaluation of novel inducible promoter/repressor systems for recombinant protein expression in Lactobacillus plantarum.
Microbial Cell factories, 15:50.
Klausberger, M.; Neff, S.; Tscheliessnig, R.; Nachbagauer, R.; Wohlbold, T. J.; Wilde, M.; Palmberger, D.; Krammer, F.; Jungbauer, A. Grabherr, R. (2016)
Globular head-displayed conserved influenza H1 hemagglutinin stalk epitopes confer protection against heterologous H1 virus strain.
PLoS ONE, 11(4): e0153579
Gelaye, E.; Achenbach, J. E.; Jenberie, S.; Ayelet, G.; Belay, A.; Martha Yami, M.; Loitsch, A.; Grabherr, R.; Diallo, A.; Lamien, C. E. (2016)
Molecular characterization of orf virus from sheep and goats in Ethiopia, 2008-2013.
Virology Journal, 13:34
2015
Ashjaei, K.; Palmberger, D.; Bublin, M.; Bajna, E.; Breiteneder, H.; Grabherr, R.; Ellinger, I.; Hoffmann-Sommergruber, K. (2015)
Atopic donor status does not influence the uptake of the major grass pollen allergen, Phl p 5, by dendritic cells.
J. Immunolog. Methods. 424:120-130
Grabherr, R.; Reichl, U. (2015)
Editorial: Can modern vaccine technology pursue the success of traditional vaccine manufacturing?
Biotechnology Journal, 10(5):657-658
Gelaye, E.; Belay, A.; Ayelet, G.; Jenberie, S.; Yami, M.; Loitsch, A.; Tuppurainen, E.; Grabherr, R.; Mach, L.; Diallo, A.; Lamien, C. E. (2015)
Capripox disease in Ethiopia: Genetic differences between field isolates and vaccine strain, and implications for vaccination failure.
Antiviral Research, 119:28-35
Heiss, S.; Grabherr, R.; Heinl, S. (2015)
Characterization of pCD033, a novel plasmid of Lactobacillus plantarum and generation of the plasmid free strain L. plantarum 3NSH.
Plasmid 81:9-20
Eikmeyer, F.; Heinl, S.; Marx, H.; Pühler, A.; Grabherr, R.; Schlüter, A. (2015)
Identification of oxygen-responsive transcripts in the silage inoculant Lactobacillus buchneri CD034 by RNA sequencing.
PloS One, 10(7):e0134149
2014
Grabherr, R. (2014)
The passive strategy: Increasing the force in the battle against influenza.
Biotechnology Journal, 9(12):1476-1477
Taus, C.; Windwarder, M.; Altmann, A.; Grabherr, R.; Staudacher, E. (2014)
UDP-N-acetyl-α-D-galactosamine:polypeptide N-acetylgalactosaminyl-transferase from the snail Biomphalaria glabrata – Substrate specificity and preference of glycosylation sites.
Glycoconjugate Journal, 31(9):661-670
Tauer, C.; Heinl, S.; Egger, E.; Heiss, S.; Grabherr, R. (2014)
Tuning constitutive recombinant gene expression in Lactobacillus plantarum.
Microbial Cell Factories 13:150
Maccani, A.; Hackl, M.; Leitner, C.; Steinfellner, W.; Graf, A.; Tatto, N.; Karbiener, M.; Scheideler, M.; Borth, N.; Mattanovich, D.; Kunert, R.; Borth, N.; Grabherr, R.; Ernst, W. (2014)
Identification of microRNAs specific for high producer CHO cell lines using steady-state cultivation.
Applied Microbiology and Biotechnology, 98(17):7535-7548
Palmberger, D.; Ashjaei, K.; Strell, S.; Hoffmann-Sommergruber, K.; Grabherr, R. (2014)
Minimizing fucosylation in insect cell-derived glycoproteins reduces binding to IgE antibodies from the sera of patients with allergy.
Biotechnology Journal, 9(9):1206-1214
Wilde, M.; Klausberger, M.; Palmberger, D.; Ernst, W.; Grabherr, R. (2014)
Tnao38, High Five and Sf9 - Evaluation of host virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression.
Biotechnol. Lett. 36(4):743-749
Anzengruber, J.; Pabst, M.; Neumann, L.; Sekot, G.; Heinl, S.; Grabherr, R.; Altmann, F.; Messner, P.; Schäffer, C. (2014)
Protein O-glycosylation in Lactobacillus buchneri.
Glycoconjugate Journal, 31(2):117-131
2013
Klausberger, M.; Wilde, M.; Palmberger, D.; Hai, R.; Albrecht, R. A.; Margine, I.; Hirsh, A.; Grabherr, R.; Krammer, F. (2013)
One-shot vaccination with an insect cell-derived low-dose influenza A H7 virus-like particle preparation protects mice against challenge.
Vaccine, 32(3):355-362
Gelaye, E.; Lamien, C. E.; Silber, R.; Tuppurainen, E. S. M.; Grabherr, R.; Diallo, A. (2013)
Development of a Cost-effective Method for Capripoxvirus Genotyping using Snapback Primer and dsDNA Intercalating Dye.
PLoS ONE, 8(10):e75971
Taus, C.; Lucini, C.; Sato, T.; Furukawa, K.; Grabherr, R.; Staudacher, E. (2013)
Expression and characterization of the first snail-derived UDP-N-acetyl-α-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase.
Glycoconjugate Journal, 30:825-833
Maccani, A.; Ernst, W.; Grabherr, R. (2013)
Whole genome sequencing improves estimation of nuclear DNA content of Chinese hamster ovary cells.
Cytometry A, 83(10):893-895
Eikmeyer, F.; Köfinger, P.; Poschenel, A.; Jünemann, S.; Zakrzewski, M.; Heinl, S.; Mayrhuber. E.; Grabherr, R.; Pühler, A.; Schwab, H.; Schlüter, A. (2013)
Influence of the inoculant Lactobacillus buchneri CD034 on the microbial community involved in the grass ensiling process.
J. Biotechnology 167(3):334-343
Gerster, P.; Kopecky, E.-M.; Hammerschmidt, N.; Klausberger, M.; Krammer, F.; Grabherr, R.; Mersich, C.; Paril, T.; Nobauer, K.; Razzazi-Fazeli, E.; Jungbauer, A. (2013)
Purification of infective baculoviruses by monoliths.
J. Chromatography A.; 1290:36-45
Mairhofer, J.; Grabherr, R. (2013)
Selection by RNA/RNA interaction: maximally minimized plasmids. Minicircle and Miniplasmid DNA Vectors. The future of non-viral and viral gene transfer.
Ed. Schleef.; Wiley-VCH. Wiley-Blackwell.; ISBN: 978-527-32456-9
2012
Palmberger, D.; Klausberger, M.; Berger, I.; Grabherr, R. (2012)
MultiBac turns sweet.
Bioengineered, 4(2):78–83
Spath, K.; Heinl, S.; Grabherr, R. (2012)
Direct cloning in Lactobacillus plantarum: Electroporation with non-methylated plasmid DNA enhances transformation efficiency and makes shuttle vectors obsolete.
Microbial Cell Factories, 11:141
Heinl, S.; Wibberg, D.; Eikmeyer, F.; Szczepanowski, R.; Blom, J.; Linke, B.; Alexander Goesmann, A.; Grabherr, R.; Schwab, H.; Pühler, A.; Schlüter, A. (2012).
Insights into the completely annotated genome of Lactobacillus buchneri CD034, a strain isolated from stable grass silage.
J. Biotechnology. 161(2):153-166
Ribeiro, S.; Mairhofer, J.; Madeira, C.; Diogo, M.; Lobato de Silva, C.; Monteiro, G.; Grabherr, R.; Cabral, J. M. (2012)
Size does matter-Plasmid DNA size does affect non-viral gene delivery efficiency in stem cells.
Cellular Reprogramming, 14(2):130-137
Palmberger, D.; Wilson, I. B. H.; Berger, I.; Grabherr, R.; Rendic, D. (2012)
SweetBac: A new approach for the production of mammalianised glycoproteins in insect cells.
PloS One, 7(4):e34226
Baumann, M.; Höppner, M. P.; Meier, M.; Pontiller, J.; Ernst, W.; Grabherr, R.; Mauceli, E.; Grabherr, M. (2012)
Artificially designed promoters: understanding the role of spatial features and canonical binding sites in transcription.
Bioengineered bugs, 3(2):120-123
2011
Spath, K.; Heinl, S.; Egger, E.; Grabherr, R. (2011)
L. plantarum and L. buchneri as expression systems: Evaluation of different origins of replication for the design of suitable shuttle vectors.
Molecular Biotechnology, 52(1):40-48
Heinl, S.; Spath, K.; Egger, E.; Grabherr, R. (2011)
Sequence analysis and characterization of two cryptic plasmids derived from Lactobacillus buchneri CD034.
Plasmid. 66(3):159-168
Grabherr, G. M.; Pontiller, J.; Mauceli, E.; Ernst, W.; Baumann, M.; Biagi, T.; Swofford, R.; Russell, P.; Zody, M. C.; Di Palma, F.; Lindblad-Toh, K.; Grabherr R. (2011)
Exploiting nucleotide composition to engineer promoters.
PLoS ONE, 6(5):e20136
Palmberger, D.; Dubravko, R.; Tauber, P.; Krammer, F.; Wilson, I. B. H.; Grabherr, R. (2011)
Insect cells for antibody production: evaluation of an efficient alternative.
J. Biotechnology, 153(3-4):160-666
Heinl, S.; Hartinger, D.; Tamhesl, M.; Schatzmayer, G.; Moll, W.-D.; Grabherr, R. (2011)
An aminotransferase from bacterium ATCC 55552 deaminates fumonisin B1.
Biodegradation, 22:25-30
2010
Krammer, F.; Pontiller, J.; Tauer, C.; Palmberger, D.; Maccani, A.; Baumann, M.; Grabherr, R. (2010)
Evaluation of the influenza A replicon for transient expression of recombinant proteins in mammalian cells.
PloS ONE, 5(10):e13265
Mäkelä, A. R.; Ernst, W.; Grabherr, R.; Oker-Blom, C. (2010)
Creation of baculovirus display libraries.
Cold Spring Harb. Protoc.; PMID: 20194462
Mäkelä, A. R.; Ernst, W.; Grabherr, R.; Oker-Blom, C. (2010)
Determination of recombinant baculoviral display viral titer.
Cold Spring Harb. Protoc.; PMID: 20194463
Mäkelä, A. R.; Ernst, W.; Grabherr, R.; Oker-Blom, C. (2010)
Immunofluorescence analysis of baculovirus-displayed viral proteins on infected insect cells.
Cold Spring Harb. Protoc.; PMID: 20194464
Mäkelä, A. R.; Ernst, W.; Grabherr, R.; Oker-Blom, C. (2010)
Immunoelectron microscopy analysis of recombinant baculovirus display viruses.
Cold Spring Harb. Protoc.; PMID: 20194465
Mäkelä, A. R.; Ernst, W.; Grabherr, R.; Oker-Blom, C. (2010)
Monitoring baculovirus-mediated efficiency of gene delivery.
Cold Spring Harb. Protoc.; PMID: 20194466
Mäkelä, A. R.; Ernst, W.; Grabherr, R.; Oker-Blom, C. (2010)
Baculovirus-based display and gene delivery systems.
Cold Spring Harb. Protoc.; PMID: 20194476
Krammer, F.; Grabherr, R. (2010)
Alternative Influenza Vaccines Made by Insect Cells.
Trends in Molecular Medicine, 16(7):313-20
Hartinger, D.; Heinl, S, Schwartz, H.; Grabherr, R.; Schatzmayr, G.; Haltrich, D.; Moll, W.-D. (2010)
Enhancement of solubility in Escherichia coli and purification of an aminotransferase from Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B(1).
Microbial cell factories, 9:62
Grabherr, R.; Ernst, W. (2010)
Baculovirus for Eukaryotic Protein Display.
Current Gene Therapy, 10:195-200
Krammer, F.; Schinko, T.; Messner, P.; Palmberger, D.; Ferko, B. and Grabherr, R. (2010)
Influenza virus-like particles as antigen-carrier platform for an ESAT-6 epitope of Mycobacterium tuberculosis.
J. Virological Methods. 167(1):17-22
Striedner, G.; Pfaffenzeller, I.; Luchner, M.; Nemecek, S.; Grabherr, R.; Bayer K. (2010)
Plasmid-free T7-based Escherichia coli expression systems.
Biotechnol Bioeng. 105(4):786-794
Krammer, F.; Palmberger, D.; Tauer, C.; Messner, P.; Grabherr, R. (2010)
Trichoplusia ni cells (high-five) are highly efficient for the production of Influenza A virus-like particles.; a comparison of two insect cell lines as production platforms for influenza vaccines.
Molecular Biotechnology, 45(3):226-234
Mairhofer J.; Cserjan-Puschmann M.; Noebauer K.; Razzazi-Fazeli E.; Striedner G.; Grabherr R. (2010)
Marker-free plasmids for gene therapeutic applications-Lack of antibiotic resistance gene substantially improves the manufacturing process.
Journal of Biotechnology, 146(3):130-137
Krammer, F.; Nakowitsch, S.; Messner, P.; Palmberger, D.; Ferko, B.; Grabherr. R. (2010)
Swine-origin pandemic H1N1 influenza virus-like particles produced in insect cells induce hemagglutination inhibiting antibodies in BALB/c mice.
Biotechnology Journal, 5(1):17-23
Heinl, S.; Hartinger, D.; Thamhesl, M.; Kunz-Vekiru, E.; Krska, R.; Schatzmayer, G.; Moll, W.-D.; Grabherr, R. (2010)
Degradation of fumonisin B1 by the consecutive action of two bacterial enzymes.
Journal of Biotechnology, 145:120-129
2008
Mairhofer, J.; Grabherr, R. (2008)
Rational vector design for efficient non-viral Gene Delivery: Challenges facing the use of plasmid DNA.
Molecular Biotechnology, 39(2):97-104
Mairhofer, J.; Pfaffenzeller, I.; Merz, D.; Grabherr, R. (2008)
A novel antibiotic free plasmid selection system: advances in safe and efficient DNA therapy.
Biotechnology Journal, 3(1):83-89
2006
Nakowitsch, S.; Kittel, C.; Ernst, W.; Egorov, A.; Grabherr, R. (2006)
Optimization of baculovirus transduction on FreeStyleTM293 cells for the generation of influenza B/Lee/40.
Molecular Biotechnology, 34(2):157–164
Mäkelä, A.; Ernst, W.; Grabherr, R.; Oker-Blom, C. (2006)
Baculovirus-based display and gene delivery systems.
Cold Spring Harbor Laboratory Press, Gene Transfer: Delivery and Expression of DNA and RNA. Chapter 28
Ernst, W.; Schinko, T.; Spenger, A.; Oker-Blom, C.; Grabherr, R. (2006)
Improving baculovirus transduction of mammalian cells by surface display of a RGD-motif.
J. Biotechnology, 126(2):237-240
Pfaffenzeller, I.; Mairhofer, J.; Striedner, G.; Bayer, K.; Grabherr, R. (2006).
Using ColE1 derived RNA I for suppression of a bacterial encoded gene: Implication for a novel plasmid addiction system.
Biotechnology Journal, 1:675-681
Matilainen, H.; Riikonen, R.; Saloniemi, T.; Korhonen, E.; Lehtinen, T.; Hyypiä, T.; Heino, J.; Grabherr, R.; Oker-Blom, C. (2006).
Presentation of RGD Motifs on the Surface of Baculovirus Enhances Viral Binding to Human Lung Carcinoma Cells.
J. Biotechnology 125:114-126
Toellner, L.; Fischlechner, M.; Ferko, B.; Grabherr, R.; Donath, E. (2006)
Virus-coated LbL-colloids as a novel multiplex suspension array for the detection and quantification of virus-specific antibodies.
Clinical Chemistry, 52(8):1575-1583
Fischlechner, M.; Toellner, L.; Messner, P.; Grabherr, R.; Donath, E. (2006)
Virus-engineered colloidal particles-a surface display system.
Angew. Chemie Int. Ed.; 45(5):784-789
2004
Spenger, A.; Ernst, W.; Condreay, P.; Kost, T.; Grabherr, R. (2004)
Influence of promoter choice and trichostatin A treatment on expression of baculovirus delivered genes in mammalian cells.
Protein Expression and Purification, 38(1):17-23
Ojala, K.; Koski, J.; Ernst, W.; Grabherr, R.; Jones, I.; Oker-Blom, C. (2004)
Improved Display of Synthetic IgG-Binding Domains on the Baculovirus Surface.
Technology in Cancer Research & Treatment, 3(1):77-84
2003
Oker-Blom, C.; Airenne, K. J.; Grabherr, R. (2003)
Baculovirus surface display strategies: emerging tools for eukaryotic libraries and gene delivery.
Briefings in Funct. Genomics and Proteomics, 2: 244-253
Poomputsa, K.; Egorov, A.; Kittel, C.; Ernst, W.; Katinger, H.; Grabherr R. (2003)
Generation of recombinant influenza virus using baculovirus as a delivery vector.
J. Vir. Methods, 110(1):111-114
2002
Grabherr, R.; Ernst, W. (2002)
Baculovirus major envelope protein gp64 and its application in eukaryotic surface display strategies.
Recent Res. Devel. Biochem.; 3:445-457
Spenger, A.; Grabherr, R.; Töllner, L.; Katinger, H.; Ernst, W. (2002)
Altering the surface properties of baculovirus Autographa californica NPV by insertional mutagenesis of the envelope protein gp64.
Eur. J. Biochem.; 269(18):4458-4467
Marzban, G.; Grillari, J.; Reisinger, E.; Hemetsberger, T.; Grabherr, R.; Katinger, H. (2002)
Age-related alterations in the protein expression profile of C57BL/6J mouse pituitaries using proteomic tools.
Exp. Gerontology, 37(12):1451-1460
Cserjan-Puschmann, M.; Grabherr, R.; Striedner, G.; Clementschitsch, F.; Bayer K. (2002)
Integrated systems approach for the optimization of microbial recombinant fermentation processes.
BioPharm.; 15:26-34
Grabherr, R. and Bayer K. (2002)
Impact of targeted vector design on bacterial plasmid replication.
Trends Biotechnol.; 20(6):257-260
Grabherr, R.; Nilsson, E.; Striedner, G.; Bayer, K. (2002)
Stabilizing plasmid copy number to improve recombinant protein production.
Biotechnol. Bioeng.; 77(2):142-147
2001
Grabherr, R.; Bayer, K. (2001)
Vector Design Related to Bio-Processing.
Food Technology and Biotechnology, 39:265-269
Grabherr, R.; Ernst, W.; Oker-Blom, C.; Jones I. (2001)
Developments in the use of baculoviruses for the surface display of complex eukaryotic proteins.
Trends Biotechnol.; 19:231-236
Grabherr, R.; Ernst, W. (2001)
The Baculovirus Expression System as a Tool for Generating Diversity by Viral Surface Display.
Combinatorial Chemistry & High Throughput Screening, 4(2):185-192
Grillari, J.; Fortschegger, K.; Grabherr, R.; Hohenwarter, O.; Katinger, H. (2001)
Analysis of Alterations in Gene Expression after Amplification of Recombinant Genes in CHO Cells.
J. Biotechnol.; 87(1):59-65
2000
Grillari, J.; Hohenwarter O.; Grabherr, R.; Katinger H. (2000)
Subtractive Hybridization of mRNAs from Early and Senescent Endothelial Cells.
Exp. Gerontology.; 35:187-197
Ernst, W.; Spenger, A.; Toellner, L.; Katinger, H. and Grabherr, R. (2000)
Expanding Baculovirus Surface Display: Modification of the Native Coat Protein gp64 of Autographa Californica NPV.
Eur. J. Biochem. 167:4033-4039
1998
Ernst, W.; Grabherr, R.; Wegner, D.; Grassauer, A.; Katinger, H. W. D. (1998)
Baculovirus Surface Display: Construction and Screening of a Eukaryotic Epitope Library.
Nucleic Acids Research, 26(7):1718-1723
1997
Sowkas, S.; Grabherr, R.; Rüker, F.; Plückthun, A.; Scheiner, O.; Breiteneder, H. (1997)
Chaperone-Assisted Expression of Allergen Peptide-Specific T-Cell Receptors.
J. Allergy and Clinical Immunology, 99:2020
Grabherr, R.; Ernst, W.; Dobblhof-Dier, O.; Sara, M.; Katinger, H. (1997)
Expression of Foreign Proteins on the Surface of Autographa californica Nuclear Polyhedrosis Virus.
Bio Techniques, 22(4):730-735
1996
Knezevic, M.; Vorauer-Uhl, K.; Hohenwarter, O.; Steindl, F.; Grabherr, R.; Raspor, P. (1996)
Human Interleukin-2 (IL-2) Expressed by Transfected Mammalian Cells.
E. J. Physiol.; 431:227-228
1994
Zhang, Y.; Strasser, P.; Grabherr, R.; VanEtten, J. L. (1994)
Hairpin Loop Structure at the Termini of the Chlorella Virus PBCV-1 Genome.
Virology, 202:1079-1082
Engelhardt, O.; Grabherr, R.; Himmler, G.; Rüker, F. (1994)
Two-step Cloning of Antibody Variable Domains in a Phage Display Vector.
Biotechniques, 17(1):44-46
Ernst, W.; Grabherr, R.; Katinger, H. (1994)
Direct Cloning into the Autographa californica Nuclear Polyhedrosis Virus Genome for the Generation of Recombinant Baculovirus.
Nucleic Acids Research, 22:2855-2856
1993
Nelson, M.; Zhang, Y.; Steffens, D. L.; Grabherr, R.; VanEtten, J. L. (1993)
Sequencing Two DNA Templates in Five Channels by Digital Compression.
Proc. Natl. Acad. Sci. USA, 90(5):1647-1651
1992
Grabherr, R.; Strasser, P.; VanEtten, J. L. (1992)
The DNA Polymerase Gene from Chlorella Viruses PBCV-1 and NY-2A Contains an Intron with Nuclear Splicing Sequences.
Virology, 188(2):721-731
Nelson, M.; VanEtten, J. L.; Grabherr, R. (1992)
DNA Sequencing of Four Bases Using Three Lanes.
Nucleic Acids Research, 20(6):1345-1348
1990
Shields, S.; Burbank, D.; Grabherr, R.; VanEtten, J. L. (1990)
Cloning and Sequencing the Cytosine Methyltransferase Gene M.CviJI from Chlorella Virus Il-3A.
Virology, 176:16-27
