[Translate to English:] Photo by <a href="https://unsplash.com/@nickshuperdesign?utm_content=creditCopyText&utm_medium=referral&utm_source=unsplash">Nick Design</a> on <a href="https://unsplash.com/photos/a-long-line-of-green-and-white-balls-ty1ZvLiwgHA?utm_content=creditCopyText&utm_medium=referral&utm_source=unsplash">Unsplash</a>

[Translate to English:] Photo by Nick Design on Unsplash

About the Stadlmann Lab

In our research group, we investigate the fundamental biological functions of protein glycosylation – a process in which sugar molecules are attached to proteins. This mechanism regulates key cellular functions such as signal transduction, cell adhesion, and intracellular transport.

Using advanced glycoproteomics technologies, we analyze the dynamic and position-specific glycosylation of proteins. Our goal is to understand how these modifications influence protein function and mediate interactions between cells and their environment. By deciphering the complexity of the glycoproteome, we aim to address fundamental biological questions, such as how glycosylation regulates cellular communication, development, and adaptation across different systems.

In addition to our research, we place great emphasis on education and training. We view our laboratory as a space where practical skills in biochemical methods are taught. Students have the opportunity to gain hands-on laboratory experience through curiosity-driven research projects. By supporting their individual projects, we foster their development into independent and creative scientists. 

We offer the opportunity to implement your own ideas in laboratory projects – either individually or in collaboration with other students. Project proposals for bachelor's and master's theses are welcome and will be further developed in close cooperation with the students.

Home of the SugarQBits

SugarQBits is a collection of software tools developed in the Stadlmann Lab. The individual SugarQBits are stand-alone scripts implemented in the cross-platform programming language Perl. These tools can be freely combined to process MS/MS data in the  .mgf file format.

Selected Publications

  1. Helm J, Mereiter S, Oliveira T, Gattinger A, Markovitz DM, Penninger JM, Altmann F, Stadlmann J., Non-targeted N-glycome profiling reveals multiple layers of organ-specific diversity in mice Nat Commun. 2024 Nov 9;15(1):9725. doi: 10.1038/s41467-024-54134-z.
  2. Stadlmann J, Taubenschmid J, Wenzel D, Gattinger A, Dürnberger G, Dusberger F, Elling U, Mach L, Mechtler K, Penninger JM., Comparative glycoproteomics of stem cells identifies new players in ricin toxicity Nature. 2017 Sep 28;549(7673):538-542. doi: 10.1038/nature24015. Epub 2017 Sep 20.
  3. Taubenschmid J, Stadlmann J, Jost M, Klokk TI, Rillahan CD, Leibbrandt A, Mechtler K, Paulson JC, Jude J, Zuber J, Sandvig K, Elling U, Marquardt T, Thiel C, Koerner C, Penninger JM., A vital sugar code for ricin toxicity Cell Res. 2017 Nov;27(11):1351-1364. doi: 10.1038/cr.2017.116. Epub 2017 Sep 19.
  4. Stadlmann J, Hoi DM, Taubenschmid J, Mechtler K, Penninger JM., Analysis of PNGase F-Resistant N-Glycopeptides Using SugarQb for Proteome Discoverer 2.1 Reveals Cryptic Substrate Specificities Proteomics. 2018 Jul;18(13):e1700436. doi: 10.1002/pmic.201700436. Epub 2018 Jun 10.
  5. Wirnsberger G, Zwolanek F, Stadlmann J, Tortola L, Liu SW, Perlot T, Järvinen P, Dürnberger G, Kozieradzki I, Sarao R, De Martino A, Boztug K, Mechtler K, Kuchler K, Klein C, Elling U, Penninger JM., Jagunal homolog 1 is a critical regulator of neutrophil function in fungal host defense Nat Genet. 2014 Sep;46(9):1028-33. doi: 10.1038/ng.3070. Epub 2014 Aug 17.
  6. Hagelkruys A, Wirnsberger G, Stadlmann J, Wöhner M, Horrer M, Vilagos B, Jonsson G, Kogler M, Tortola L, Novatchkova M, Bönelt P, Hoffmann D, Koglgruber R, Steffen U, Schett G, Busslinger M, Bergthaler A, Klein C, Penninger JM., A crucial role for Jagunal homolog 1 in humoral immunity and antibody glycosylation in mice and humans J Exp Med. 2021 Jan 4;218(1):e20200559. doi: 10.1084/jem.20200559.
  7. Raglow Z, McKenna MK, Bonifant CL, Wang W, Pasca di Magliano M, Stadlmann J, Penninger JM, Cummings RD, Brenner MK, Markovitz DM., Targeting glycans for CAR therapy: The advent of sweet CARs Mol Ther. 2022 Sep 7;30(9):2881-2890. doi: 10.1016/j.ymthe.2022.07.006. Epub 2022 Jul 12.
  8. Capraz T, Kienzl NF, Laurent E, Perthold JW, Föderl-Höbenreich E, Grünwald-Gruber C, Maresch D, Monteil V, Niederhöfer J, Wirnsberger G, Mirazimi A, Zatloukal K, Mach L, Penninger JM, Oostenbrink C, Stadlmann J., Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptor Elife. 2021 Dec 20;10:e73641. doi: 10.7554/eLife.73641.
  9. Yan S, Vanbeselaere J, Ives C, Stenitzer D, Nuschy L, Wöls F, Paschinger K, Fadda E, Stadlmann J, Wilson IBH. Glycoproteomic and Single-Protein Glycomic Analyses Reveal Zwitterionic N-Glycans on Natural and Recombinant Proteins Derived From Insect Cells. Mol Cell Proteomics. 2025 May 5;24(6):100981. doi: 10.1016/j.mcpro.2025.100981. Epub ahead of print. PMID: 40334746; PMCID: PMC12166434.
  10. Walcher S, Hager-Mair FF, Stadlmann J, Kählig H, Schäffer C., Deciphering fucosylated protein-linked O-glycans in oral Tannerella serpentiformis: Insights from NMR spectroscopy and glycoproteomics Glycobiology. 2024 Dec 10;34(12):cwae072. doi: 10.1093/glycob/cwae072.