772401 Basic course I - analysis, design and engineering of proteins (in Eng.)


Art
Vorlesung
Semesterstunden
2
Vortragende/r (Mitwirkende/r)
Organisation
Angeboten im Semester
Wintersemester 2019/20
Unterrichts-/ Lehrsprachen
Englisch

Lehrinhalt

1a: Chemistry of non-covalent interactions and structural building blocks in proteins (Haltrich)
1b: Protein diversity – evolution of structure and function (Haltrich)
1c: Protein structure I: X-ray crystallography (Djinovic-Carugo)
1d: Protein structure II: Cryo-EM (Heuser)
1e: Protein structure III: Electronic circular dichroism, fluorescence and Fourier transform infrared spectroscopy of proteins (Obinger)
1f: Protein structure IV: Self-assembling proteins - Light scattering, electron microscopy, atomic force microscopy (Schäffer)
1g: Measurement of protein – ligand (protein) interaction: isothermal titration calorimetry and surface plasmon resonance (Schaffner)
1h: Core facility Biomolecular & Cellular Analysis – practical considerations (Schaffner)
1i: Folding pathways, conformational and thermal stability of proteins (Obinger)
1j: Measurement of changes in stability – differential scanning calorimetry and more (Obinger)
1k: Enzyme kinetics: steady–state versus presteady–state kinetics (Ludwig)
1l: Mutagenesis: Site–directed mutagenesis, directed evolution by non–recombinative and recombinative methods (Peterbauer)
1m: Protein libraries: design and construction (Rüker)
1n: Surface display technologies (Rüker)
1o: Screening criteria and methods (Peterbauer)
1p: Case study: Engineered therapeutic antibodies (Rüker)

Inhaltliche Voraussetzungen (erwartete Kenntnisse)

Study in biotechnology, food sciences and technology, (bio)chemistry, molecular biology, medicine or bioinformatics
.

Lehrziel

Improved knowledge of structural biology of proteins, conformational and thermal stability, as well as (un-)folding pathway(s). Profound understanding of methods in analysis of protein structure, quality and stability.
Understanding of design of protein libraries, methods in mutagenesis, surface display technologies and screening strategies. Knowledge of methods for protein engineering and studies in enzyme kinetics and protein – ligand (protein) interaction.
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