953327 Laboratory diagnosis of plant damages (in Eng.)


Art
Übung
Semesterstunden
2
Vortragende/r (Mitwirkende/r)
Baumgartner, Susanne , Wieczorek, Krzysztof
Organisation
Angeboten im Semester
Wintersemester 2019/20
Unterrichts-/ Lehrsprachen
Englisch

Lehrinhalt

Short overview of the protein extraction from plant tissue and protein detection using specific antibodies: ELISA immunoassay to detect plant viruses in plant tissues.
Short overview of the extraction of DNA/RNA from plant tissue and RNA/DNA detection: RT-PCR-based detection of plant viruses.
DNA fingerprinting of different Phytophthora spp.: PCR and digestion of the PCR product with restriction enzymes. DNA detection via gel electrophoresis.


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Inhaltliche Voraussetzungen (erwartete Kenntnisse)

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Lehrziel

Basic knowledge of antibody-based ELISA test as a tool to detect plant pathogenes.
Basic knowledge of PCR-based detection of plant pathogenes.
Students can perform ELISA test and PCR to detect plant pathogenes.
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.