773311 Introduction into crystallography and NMR spectroscopy of proteins (in Eng.)


Art
Vorlesung
Semesterstunden
2
Vortragende/r (Mitwirkende/r)
Organisation
Angeboten im Semester
Sommersemester 2020
Unterrichts-/ Lehrsprachen
Englisch

Lehrinhalt

Structural features of amino acids, peptides and proteins
Theory of x-ray diffraction from crystals
Theory of crystallization, practical aspects
Serial femtosecond X-ray crystallography (SFX)
Data collection, phasing, molecular replacement and structure refinement/validation
Databases and case studies
Basic principles of NMR spectroscopy
Multidimensional NMR
NMR Structure determination (assignment, Noe)
Isotope labeling of proteins, assignment strategies, residual dipolar couplings
Model building, structure refinement and validation
Protein Dynamics (Relaxation phenomena)
Protein-ligand structures (TrNOe, STD), Intrinsically disordered proteins
Comparison of X-ray and NMR structures
Cryo-electron microscopy

Inhaltliche Voraussetzungen (erwartete Kenntnisse)

Basic knowledge of biochemistry and spectroscopy. The lecture is recommended for advanced master study students and PhD students
.

Lehrziel

The students will have obtained a basic knowledge in structural biology. In particular, they should be able to assess the quality of published protein structural data and models and get hands-on experience in using 3-D models from the protein database
.
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.