953318 Gene technology for plant pathologists (in Eng.)


Art
Vorlesung
Semesterstunden
2
Vortragende/r (Mitwirkende/r)
Wieczorek, Krzysztof , Hage-Ahmed, Karin
Organisation
Angeboten im Semester
Wintersemester 2020/21
Unterrichts-/ Lehrsprachen
Englisch

Lehrinhalt

Gene technology for plant pathologists lecture deals with different molecular biology aspects and methods currently relevant in the crop production and protection such as: plant resistance, cloning, production and use of transgenic plants to improve their resistance against pathogens and pests, different methods of plant genome engineering etc.

Inhaltliche Voraussetzungen (erwartete Kenntnisse)

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Lehrziel

Students gain knowledge of:

-Basic genetic cloning methods including PCR, restriction enzymes, different plasmids and vectors etc.
-Plant transformation tools, selection markers and reporter genes.
-Examples of transgenic plants in the field.
-Resistance genes and host resistance.
-Technics of plant genome engineering including RNAi, ZFN, TALENs and CRISP/Cas9.
-Mechanisms of plant resistance against different pests and pathogens including induced resistance, systemic acquired resistance, induced systemic resistance, ETI and PTI etc.
-The role of secondary metabolites in the plant resistance.

Learning outcomes:

Upon completion of this course, the students will be able to:
- name and explain the principles of gene cloning including the PCR, digestion with restriction enzymes, ligation and selection of transformed bacteria using different systems
- name and explain the different plant resistant mechanisms including induced resistance, systemic acquired resistance, induced systemic resistance, ETI and PTI etc.
- name the different methods of plant genome engineering
- name and explain the role of different substances in the plant resistance such as phytoalexins, phytoanticipins and different secondary metabolites
- name the transformation tools such as Agrobacterium-mediated transformation and their role in plant-pathogen studies
- name examples of selection markers and reporter genes- name and describe examples of transgenic plants in the field and assess their advantages and disadvantages
- name and explain principles of resistance genes, classes of resistance genes and the location of the corresponding proteins
- name and explain principles of perception of pathogen effectors in plants and host resistance
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