752311 Biochemical reaction engineering (in Eng.)


Art
Vorlesung
Semesterstunden
2
Vortragende/r (Mitwirkende/r)
Ludwig, Roland
Organisation
Angeboten im Semester
Wintersemester 2020/21
Unterrichts-/ Lehrsprachen
Englisch

Lehrinhalt

The central theme of this lecture is the application of enzymes in biocatalysis and the design of an enzymatic process. The following aspects of biochemical reaction engineering will be discussed:

1. Course overview and introduction
2. Biotechnology, biotransformations, catalysis,…
2.1. Additional reading – enzyme evolution
3. Biocatalysis
4. Enzyme applications
4.1. Additional reading – flavoprotein oxidases
4.2. Additional reading – C-C hydrolases
5. Exercise 1
6. Biochemical reaction engineering, biocatalyst screening and engineering
6.1. Additional reading – Accelerating the implementation of biocatalysts in industry
7. Enzymatic activity, diffusion, collisions and transitions
8. Enzyme kinetics (microkinetics)
8.1. Additional reading – Die Kinetik der Invertinwirkung
9. Inhibition, multi-reactant systems
10. Substrate specificity, enantioselectivity,…
11. Exercise 2
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12. Macrokinetics
12.1. Additional reading – considerations when measuring biocatalyst performance
13. Reactor types
13.1. Additional reading – cascading microreactors
13.2. Additional reading – flow bioreactors
14. Scale-up, process control, process monitoring
15. Exercise 3
16. Enzymatic redox transformations
16.1. Additional reading – multi-enzyme processes
17. Exercise 4
18. Immobilisation vs. enzyme membrane reactor concept
18.1. Additional reading – immobilized enzymes
19. Enzyme stability and deactivation
19.1. Additional reading – kinetic stability of proteins
19.2. Additional reading – calulating TTN in continuous processes
20. Solvents and environmental impact of processes
20.1. Additional reading – on the relationship between enantioselectivity and enzyme
flexibility
20.2. Additional reading – enzyme-catalyzed processes in organic solvents
21. Green chemistry
21.1. Additional reading – catalysis, green chemistry and process design
21.2. Additional reading – E-factors
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Please check BOKUlearn for more information and the Zoom links.

Inhaltliche Voraussetzungen (erwartete Kenntnisse)

Participants will have to recall their knowledge from different lectures and disciplines in the field of biotechnology and create new connections.
.

Lehrziel

After the positive completion of the course the students can:

1) Describe essential concepts of reaction kinetics (mass balance, mass transfer) and reactor types
2) Recall and explain important catalytic constants (Km, kcat,...) and process parameters (T, pH, [S], [P], [I],,...) which affect enzymatic reactions and their applications in industrial scale processes
3) Derive and interprete process diagrams (substrate conversion, product formation, reaction rate,...) and performance numbers (space-time yield, specific productivity, total turnover number,...)
4) Apply fundamental principles of biocatalysis for the engineering of a biochemical reaction
5) Asses and argue the influence of catalytic constants on enzymatic processes
6) Generate solutions for possible problems caused by non-ideal enzymes and poor reaction conditions
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.