790397 Protein engineering (in Eng.)

Vortragende/r (Mitwirkende/r)
Rüker, Florian , Wozniak-Knopp, Gordana , Peterbauer, Clemens Karl
Angeboten im Semester
Wintersemester 2020/21
Unterrichts-/ Lehrsprachen


MO09.11.202014:3017:001GWK FR Antibodies
DI10.11.202014:3017:002GWK FR Antibodies
MI11.11.202014:3017:003GWK Design of alternative binding scaffolds
DO12.11.202014:3017:004CP Enzyme evolution
FR13.11.202014:3017:005CP Enzyme evolution
MO16.11.202014:3017:006FR Top 50 Drugs
DI17.11.202014:3017:007GWK CP Technology of library construction
MO23.11.202014:3017:008GWK Difficult-to-express proteins
DI24.11.202014:3017:009FR Covid19 vaccines in development
MI25.11.202014:3017:0010CP High throughput screening / microfluidics
DO26.11.202014:3017:0011GWK Fusion proteins
FR27.11.202014:3017:0012GWK CP FR Wrap up and exam

•Influenza / HIV / HPV
•Difficult-to-express proteins
•Fusion proteins
•Covid19 vaccines in development
•High throughput screening / microfluidics
•Enzyme evolution
•Technology of library construction
•Design of alternative binding scaffolds

Inhaltliche Voraussetzungen (erwartete Kenntnisse)

Basic knowledge in molecular biology and biochemistry
Protein structure and function


Lecture 1: Antibodies
Learning objectives:
-To describe the antibody fold, antibody domain structure and molecular architecture
-To define the beneficial biological properties of antibodies and relate them to molecular structure
-To comment on the importance of antibody-based therapeutics in present times

Lectures 2 and 3: anti-viral Vaccines
Learning objectives:
-To describe various approaches how protein engineering helps to design better vaccines
-To introduce rational, computational, and combinatorial strategies for vaccine design
-To define the requirements for successful vaccines and how these can be met by protein engineering approaches
-To discuss and understand selected examples for vaccines that are based on protein engineering

Lecture 4: Difficult-to-express proteins
Learning objectives:
-To describe the properties of Difficult-to-express proteins as well as their role as therapeutic targets
-To evaluate various approaches and methods to improve solubility, folding, enable crystallization and prevent aggregation and formation of inclusion bodies

Lecture 5: Fusion proteins
Learning objectives:
-To comment on the advantages of fusion proteins intended for therapeutic purposes, bioanalytics and industrial applications
-To compare different designs of fusion proteins and corresponding tags
-To discuss challenges in expression, production and analytics of fusion proteins

Lecture 6: Covid19 vaccines in development
Learning objectives:
-To describe which types of vaccines against Covid19 are currently in development
-To discuss the different strategies and approaches

Lecture 7: High throughput screening / microfluidics
Learning objectives:
- To understand the principal characteristics of enzyme activity assays: localization of enzyme, nature of substrate and product, detectable and quantifyable molecules
- Knowledge of factors that influence the detection of a product/byproduct/secondary product of an enzymatic reaction and its link to the genotype

Lecture 8: Enzyme evolution
Learning objectives:
- To understand the drivers of evolution of enzyme activity: mutation and recombination, selective pressure, trade-offs between stability and dynamics
- To describe the acquisition of novel functions and reactivities

Lecture 9 and 10: Technology of library construction
Learning objectives:
-To describe the concept of library display and selection
-To compare various library display systems
-To choose a proper mutagenesis method for the intended protein modification
-To design a selection process protocol for the desired change of protein function

Lecture 11: Design of alternative binding scaffolds
Learning objectives:
-To identify the key properties of a functional binding scaffold
-To implement the knowledge on protein structure in designing a binding surface
-To apply the principles of library selection methods to the design of a binding scaffold
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