953327 Laboratory diagnosis of plant damages (in Eng.)


Art
Übung
Semesterstunden
2
Vortragende/r (Mitwirkende/r)
Wieczorek, Krzysztof , Hage-Ahmed, Karin , Neubacher, Katharina , Wieczorek, Richard
Organisation
Angeboten im Semester
Sommersemester 2021
Unterrichts-/ Lehrsprachen
Englisch

Lehrinhalt

Short overview of the protein extraction from plant tissue and protein detection using specific antibodies: ELISA immunoassay to detect plant viruses in plant tissues.
Short overview of the extraction of DNA/RNA from plant tissue and RNA/DNA detection: RT-PCR-based detection of plant viruses.
DNA fingerprinting of different Phytophthora spp.: PCR and digestion of the PCR product with restriction enzymes. DNA detection via gel electrophoresis.


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Inhaltliche Voraussetzungen (erwartete Kenntnisse)

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Lehrziel

Upon successful completion of this lecture, students will be able to…

1) explain methods of laboratory diagnostics (sandwich-ELISA, RT-PCR detection, PCR-RFLP-determination) for the identification of various plant pathogens.

2) test plant tissue for plant phytopathogenic viruses using ELISA assay.

3) test plant tissue for plant phytopathogenic viruses using PCR detection.

4) carry out DNA fingerprinting to identify different Phytophthora species.

5) independently and carefully perform laboratory diagnostic methods learned in the course (extraction of DNA and RNA, PCR-based tests and immunological detection assays).
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.