940413 Instructional course IIC - cell imaging (in Eng.)


Art
Übung
Semesterstunden
2
Vortragende/r (Mitwirkende/r)
Pum, Dietmar , Schoberer, Jennifer , Schosserer, Markus , Arcalis, Elsa , Schäffer, Christina
Organisation
Angeboten im Semester
Sommersemester 2022
Unterrichts-/ Lehrsprachen
Englisch

Lehrinhalt

Use of fluorescent protein tags as reporters for subcellular localization, expression of fluorescently-tagged marker proteins in different subcellular compartments in plants and mammalian cells. Localisation and tracking of proteins to follow subcellular trafficking.
Methods to be applied include live-cell imaging, confocal laser scanning microscopy, quantitative co-localization studies, measurement of protein dynamics using fluorescence recovery after photobleaching or photoactivatable fluorescent proteins, monitoring of in vivo protein-protein interactions using fluorescent protein techniques like biomolecular fluorescence complementation. Fixation and embedding of samples for electron microscopy, ultramicrotomy, basic electron microscopy methods, negative staining, immunolocalisation methods, electron tomography.

Lehrziel

Students will be familiar with basic and cutting edge light and electron microscopy techniques. Increased understanding of the subcellular organization and secretory pathway of eukaryotic cells.
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.