790417 Instructional course IVC - protein purification (in Eng.)


Art
Übung
Semesterstunden
2
Vortragende/r (Mitwirkende/r)
Organisation
Angeboten im Semester
Sommersemester 2022
Unterrichts-/ Lehrsprachen
Englisch

Lehrinhalt

An introduction to the governing principles of preparative chromatography, membrane filtration and precipitation/flocculation with respect to separation of proteins and other macromolecules will be given. Relevant methods will be described for the purification of proteins from different starting materials such as microbial cells, mammalian cells and plant tissue, in particular when a purification tag is not available. Also a detailed insight into available purification tags will be provided, and their advantages and disadvantages will be discussed.

Inhaltliche Voraussetzungen (erwartete Kenntnisse)

none

Lehrziel

The course will provide instructions on how to optimize a purification scheme, set-up a de novo purification protocol without prior knowledge of protein properties and how to scale up a protein purification method. The students will be trained in trouble shooting of chromatography, membrane filtration, precipitation/flocculation and strategies to avoid protein degradation during purification. Students will be able to select purification tags on a rational basis
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