940402 Basic course II - biosynthesis, post-translational modifications and trafficking of recombinant proteins (in Eng.)

Vortragende/r (Mitwirkende/r)
Stöger, Eva , Mach, Lukas , Gasser, Brigitte , Stadlmann, Johannes , Wilson, Iain B.H. , Strasser, Richard
Angeboten im Semester
Sommersemester 2023
Unterrichts-/ Lehrsprachen


Part A: Biosynthesis and posttranslational modifications of recombinant proteins

Biosynthesis of cytosolic and secretory proteins
Protein folding, quality control and co-factor incorporation (heme, flavin) within the cytosol and the secretory pathway
Posttranslational protein modifications I: N- and O-glycosylation in biotechnologically relevant organisms
Posttranslational protein modifications II: role of glycosylation in targeting, turnover and immunology of recombinant proteins
Posttranslational protein modifications III: phosphorylation, hydroxylation, carboxylation, acetylation, methylation etc. and their functional relevance
Analysis of post-translational modifications I: chromatographic and electrophoretic techniques
Analysis of post-translational modifications II: mass spectrometry
Proteomics: concepts and recent developments

Part B: Intracellular protein trafficking and its biotechnological relevance

General concepts of intracellular transport processes
The secretory pathway I: the endoplasmic reticulum
The secretory pathway II: the Golgi apparatus
Protein secretion in yeasts and filamentous fungi
Regulated and non-canonical secretion
Endocytosis, endosomes and lysosomes
Intracellular protein degradation pathways
Protein storage organelles

Inhaltliche Voraussetzungen (erwartete Kenntnisse)

Basic knowledge in protein biochemistry and molecular cell biology.


Improved knowledge of protein biosynthesis and post-translational protein modifications and their functionalities. Profound understanding of the relevance of post-translational protein modifications for the production and application of recombinant proteins. Comprehensive knowledge of eukaryotic expression systems with respect to protein secretion, endocytosis and turnover.
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