790031 3D cell culture formats for generation of complex tissue engineering products and disease model application (in Eng.)

Art
Vorlesung
Semesterstunden
1
Vortragende/r (Mitwirkende/r)
Organisation
Angeboten im Semester
Sommersemester 2023
Unterrichts-/ Lehrsprachen
Englisch
Lehrinhalt

In the course, different classes of biomaterials and their medical applications will be presented. A particular focus of the lecture is on materials such as hydrogels. Hydrogels are highly hydrated polymer networks that closely resemble natural tissue matrix, making them ideal for cell growth. Different classes of hydrogels with tunable properties are presented.
The incorporation of stiffness and oxygen gradients into 3D hydrogel-based constructs can be a very useful tool to both restore physiological oxygen gradients in poorly vascularized tissues (e.g. cartilage) for tissue engineering, and to study pathological conditions in tumors and to recapitulate wounds. In this part of the lecture, different types of gradient hydrogels and their applications are presented.
n vitro cultivation conditions play a crucial role in cell physiology and cellular response to external stimuli. Another important cell culture parameter that is often neglected in traditional cultivation is the concentration of dissolved oxygen. In general, cell culture is performed under ambient oxygen saturation (21%), while the amount of oxygen available in human tissues in vivo is much lower (1% to 15%).
Hypoxia is an essential condition and driving force of biological processes in embryogenesis. Learning from nature, researchers must account for local oxygen tensions during cell cultivation and use controlled hypoxia for the fabrication of functional TE constructs, disease modeling, and the production of biologically active cell therapeutics.
Additionally, different types of cells modified with genetically encoded hypoxia biosensors and different two- and three-dimensional culture systems are presented and evaluated. These hypoxia reporter cells are the first of their kind to be described in the literature and enable the spatiotemporal detection of oxygen supply limitation in various in vitro systems.
Inhaltliche Voraussetzungen (erwartete Kenntnisse)

Bachelor degree/level in Biotechnology or related studies
Lehrziel

After successful completion of this lecture, students have a basic knowledge on 3 D cell culture formats and specific aspects of physiological concepts in tissue engineering. Students are able to explain the “essential composition of in vitro testing systems and strategies for development of disease models. Students have knowledge of 3 R concept (reduction, refinement, replacement) for animal testing. Furthermore, they are able to discuss ethical and technological issues when using e.g. stem cells and identify and evaluate limitations and risks with advanced cell based products
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