ULG148001 Protein Chromatography - Engineering Fundamentals and Measurements for Process Development and Scale up (in Eng.)

Vorlesung und Übung
Vortragende/r (Mitwirkende/r)
Jungbauer, Alois , Carta, Giorgio
Angeboten im Semester
Wintersemester 2023/24
Unterrichts-/ Lehrsprachen


Course director is Prof. Alois Jungbauer from Department of Biotechnology. He is an expert in downstream processing of biopharmaceuticals and process chromatography.

Course duration is one week with a preparation time and a follow up. Lecture, practical experiments, evaluation of experiments and presentation of results are the core of the programme. The scope of the course is to provide insight in the application of chromatographic theory with special emphasis on mass transfer and dispersion. The theory will cover the fundamentals, the sorption equilibria, modes of operation, types of chromatographic media, dynamics of chromatography, effects of dispersion and extra column effects. The experimental part will comprise pulse response experiments, dynamic binding capacity, shallow bed experiments, and linear gradient elutions. The experiments will carried out with typical conventional porous media exhibiting different transport mechanisms. Participants will be also trained in the pitfalls of peak fitting and evaluation of chromatographic data. Detailed course notes will be provided. At the end of the course a detailed protocol has to be worked out.

Inhaltliche Voraussetzungen (erwartete Kenntnisse)

The participants from biomanufacturing industry or supply industry must have at least bachelor’s degree in biology, biochemistry, biochemical engineering or chemical engineering. Experience in process chromatography in industry is useful, but not mandatory. Participants from academia must have at least a master’s degree in in biology, biochemistry, biochemical engineering or chemical engineering.


An introduction to the fundamental underpinnings of protein chromatography and to
advanced laboratory equipment and techniques for process development and scale-up;
• The tools to understand and troubleshoot actual bio-manufacturing processes;
An understanding of the regulatory, economic, technological, and operational
constraints affecting downstream process design and operation;
• The value of fundamentals in solving practical design problems;
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.