790397 Protein engineering (in Eng.)

Vortragende/r (Mitwirkende/r)
Wozniak-Knopp, Gordana , Peterbauer, Clemens Karl
Angeboten im Semester
Wintersemester 2023/24
Unterrichts-/ Lehrsprachen


These are the aproximate topics

•Bispecific antibodies
•Alternative binding scaffolds
•CAR-T cells
•Difficult-to-express proteins
•Fusion proteins
•High throughput screening / microfluidics
•Enzyme evolution
•Technology of library construction

Inhaltliche Voraussetzungen (erwartete Kenntnisse)

Basic knowledge in molecular biology and biochemistry
Protein structure and function


Students who successfully complete this course will be able to:
Learning objectives:
-To describe the antibody fold, antibody domain structure and molecular architecture
-To define the beneficial biological properties of antibodies and relate them to molecular structure
-To comment on the importance of antibody-based therapeutics in present times

Bispecific antibodies:
- To describe the obligate bispecific antibodies (bsAbs) and their main modes of action
- To compare the different molecular architectures of bsAbs
- To comment on engineering methods for construction of bsAbs

Design of alternative binding scaffolds
Learning objectives:
-To identify the key properties of a functional binding scaffold
-To implement the knowledge on protein structure in designing a binding surface
-To apply the principles of library selection methods to the design of a binding scaffold

CAR T-cells
Learning objectives:
-To identify the key structural elements of a CAR and relate those to CAR-T function
-To implement the knowledge on protein engineering in design of improved CARs

Difficult-to-express proteins
Learning objectives:
-To describe the properties of Difficult-to-express proteins as well as their role as therapeutic targets
-To evaluate various approaches and methods to improve solubility, folding, enable crystallization and prevent aggregation and formation of inclusion bodies

Fusion proteins
Learning objectives:
-To comment on the advantages of fusion proteins intended for therapeutic purposes, bioanalytics and industrial applications
-To compare different designs of fusion proteins and corresponding tags
-To discuss challenges in expression, production and analytics of fusion proteins

High throughput screening / microfluidics
Learning objectives:
- To understand the principal characteristics of enzyme activity assays: localization of enzyme, nature of substrate and product, detectable and quantifyable molecules
- Knowledge of factors that influence the detection of a product/byproduct/secondary product of an enzymatic reaction and its link to the genotype

Enzyme evolution
Learning objectives:
- To understand the drivers of evolution of enzyme activity: mutation and recombination, selective pressure, trade-offs between stability and dynamics
- To describe the acquisition of novel functions and reactivities

Technology of library construction
Learning objectives:
-To describe the concept of library display and selection
-To compare various library display systems
-To choose a proper mutagenesis method for the intended protein modification
-To design a selection process protocol for the desired change of protein function
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.