790397 Protein engineering (in Eng.)
- Art
- Vorlesung
- Semesterstunden
- 3
- Vortragende/r (Mitwirkende/r)
- Wozniak-Knopp, Gordana , Peterbauer, Clemens Karl
- Organisation
- Angeboten im Semester
- Wintersemester 2023/24
- Unterrichts-/ Lehrsprachen
- Englisch
- Lehrinhalt
-
These are the aproximate topics
•Antibodies
•Bispecific antibodies
•Alternative binding scaffolds
•CAR-T cells
•Difficult-to-express proteins
•Fusion proteins
•High throughput screening / microfluidics
•Enzyme evolution
•Technology of library construction
- Inhaltliche Voraussetzungen (erwartete Kenntnisse)
-
Basic knowledge in molecular biology and biochemistry
Protein structure and function
- Lehrziel
-
Students who successfully complete this course will be able to:
Antibodies
Learning objectives:
-To describe the antibody fold, antibody domain structure and molecular architecture
-To define the beneficial biological properties of antibodies and relate them to molecular structure
-To comment on the importance of antibody-based therapeutics in present times
Bispecific antibodies:
- To describe the obligate bispecific antibodies (bsAbs) and their main modes of action
- To compare the different molecular architectures of bsAbs
- To comment on engineering methods for construction of bsAbs
Design of alternative binding scaffolds
Learning objectives:
-To identify the key properties of a functional binding scaffold
-To implement the knowledge on protein structure in designing a binding surface
-To apply the principles of library selection methods to the design of a binding scaffold
CAR T-cells
Learning objectives:
-To identify the key structural elements of a CAR and relate those to CAR-T function
-To implement the knowledge on protein engineering in design of improved CARs
Difficult-to-express proteins
Learning objectives:
-To describe the properties of Difficult-to-express proteins as well as their role as therapeutic targets
-To evaluate various approaches and methods to improve solubility, folding, enable crystallization and prevent aggregation and formation of inclusion bodies
Fusion proteins
Learning objectives:
-To comment on the advantages of fusion proteins intended for therapeutic purposes, bioanalytics and industrial applications
-To compare different designs of fusion proteins and corresponding tags
-To discuss challenges in expression, production and analytics of fusion proteins
High throughput screening / microfluidics
Learning objectives:
- To understand the principal characteristics of enzyme activity assays: localization of enzyme, nature of substrate and product, detectable and quantifyable molecules
- Knowledge of factors that influence the detection of a product/byproduct/secondary product of an enzymatic reaction and its link to the genotype
Enzyme evolution
Learning objectives:
- To understand the drivers of evolution of enzyme activity: mutation and recombination, selective pressure, trade-offs between stability and dynamics
- To describe the acquisition of novel functions and reactivities
Technology of library construction
Learning objectives:
-To describe the concept of library display and selection
-To compare various library display systems
-To choose a proper mutagenesis method for the intended protein modification
-To design a selection process protocol for the desired change of protein function
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw.
finden Sie auf der Lehrveranstaltungsseite in BOKUonline.