790369 Practical course in cell culture and fermentation (in Eng.)

Art
Übung
Semesterstunden
3
Vortragende/r (Mitwirkende/r)
Borth, Nicole , Kunert, Renate , Marx, Nicolas
Organisation
Angeboten im Semester
Sommersemester 2025
Unterrichts-/ Lehrsprachen
Englisch
Lehrinhalt

IMPORTANT
Please only register if you already meet one of the requirements at the time of registration.
A prerequisite is a degree, including a registered grade, in at least one of the courses:
790351, 790318, or 790367.

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The course agenda
The course lasts eight days and comprises 3 ECTS credits.
In the first part of the course, you will learn
- how to apply transfection methods like the CRISPR-Cas system
- Handling of transient cell culture cells
- Isolation, detection, and characterization of the proteins produced using the SDS gel FACS assay and octet measurement

In the second part of the course, you will dive into the production of recombinant proteins. You will learn how to set up and program a bioreactor yourself:
- Sterile connection of the periphery
- Medium preparation and inoculation of the bioreactor with recombinant CHO cells
- Daily sampling to determine cell concentration, viability, recombinant protein concentration, nutrient and metabolite concentrations
- Adjustment of relevant culture parameters, e.g., pH, DO.
- Documentation, data analysis, and interpretation
Inhaltliche Voraussetzungen (erwartete Kenntnisse)

For Master Biotechnology or PhD students, while undergraduate students are not accepted.
Furthermore, one of the following examinations must already have been successfully completed:
Cell Factories (791351) or Cell Culture (791318) or Stem Cells and Tissue Engineering (791367)
Lehrziel

You will gain a realistic understanding of cell culture technology and the handling and operation of a bioreactor for recombinant protein production. Simple work with primary and established cell lines and critical steps will familiarize you with experimental planning, evaluation, and documentation. At the end of the course, you will present and discuss the results in front of an audience.
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.