940339 Methods in cell biology (in Eng.)

Art
Vorlesung und Übung
Semesterstunden
2
Vortragende/r (Mitwirkende/r)
Schoberer, Jennifer
Organisation
Pflanzenbiotechnologie und Zellbiologie
Angeboten im Semester
Sommersemester 2025
Unterrichts-/ Lehrsprachen
Englisch
Lehrinhalt

• 1st half: Introduction to optical methods in cell biology (three 3-hour lectures)

Lecture 1: Visualising cells
Lecture 2: Following protein dynamics in living cells
Lecture 3: Measuring physiological processes in living cells

• 2nd half: Work with various optical methods (5 hours): The practical part will give you hands-on experience in the following systems:

Inverted research microscope (Zeiss Axio Observer) equipped with:
*epifluorescence
*bright field
*polarization microscopy
*phase contrast
*differential interference contrast

Confocal microscope (Leica SP8)

Stimulated emission depletion (STED) super-resolution microscope (Leica SP8-gSTED)
Lehrziel

•You have acquired an understanding of the basic concepts behind the most important optical methods in cell biology.
•You have used a significant selection of optical cell biological tools that are currently available at the IPBT/IMPB and the BOKU Core Facility Multiscale Imaging.
•You have shown the capability to process, mount, and describe microscopic figures as required for thesis and/or journal publication.
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.