BTLW300977 Practical course in cell culture and fermentation (in Eng.)

Art
Übung
Semesterstunden
3
Vortragende/r (Mitwirkende/r)
Üzülmez, Öykü , Kunert, Renate , Hawlin, Vanessa , Borth, Nicole , Virgolini, Nikolaus
Organisation
Bioverfahrenstechnik
Angeboten im Semester
Wintersemester 2025/26
Unterrichts-/ Lehrsprachen
Englisch
Lehrinhalt

• 100% attendance is expected.
• Registration status must be fixed 3 weeks prior to course begin!
• Laboratory safety quiz must be completed in due time to have a fix position in the course.

! Check expected previous knowledge before registering !

Part "Bioprocessing of mammalian cells"
- Assembly and programming of a stirred tank bioreactor
- Sterile connection of periphery
- Medium preparation and inoculation of the bioreactor with recombinant CHO cells
- Daily sampling to determine cell concentration, viability, recombinant protein concentration, nutrient and metabolite concentrations
- Documentation and adjustment of relevant culture parameters e.g. pH, DO etc.
- Data analysis and interpretation


Part "Cell culture engineering"
Routine cell culture of CHO cells
- cell density measurement
- calculation of seeding density
- passaging
- calculation of growth rate

CRISPR/Cas9 editing:
- Transfection of CHO cells with plasmids coding for CRISPR/Cas9
- Analysis of transfection efficiency with flow cytometry
- Genotyping of DNA edits
- Staining and flow cytometry analysis of phenotypic effect
- Discussion and interpretation of results
Inhaltliche Voraussetzungen (erwartete Kenntnisse)

Applicable for MSc Biotechnologie or PhD students. BSc students can not be accepted. Prerequisite: either one of the following courses must be successfully completed:
"Cell factories (790351)" or "Animal cell culture (790318)" or "Stem cells and tissue engineering (790367)"
Lehrziel

Students shall gain a basic understanding of cell culture technology and the handling and operation of a bioreactor for recombinant protein production. They are able to carry out work with established cell lines and recognize critical work steps, such as sterile handling. Students shall get familiar with planning and dividing the workload among themselves. Further acquirable skills include, data documentation, evaluation and presentation in front of an audience.
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.