BTLW300967 Up- und downstream-processing (in Eng.)

Art
Vorlesung
Semesterstunden
3
Vortragende/r (Mitwirkende/r)
Sagar, Isita , Buyel, Johannes , Kasper, Cornelia
Organisation
Bioverfahrenstechnik
Angeboten im Semester
Wintersemester 2025/26
Unterrichts-/ Lehrsprachen
Englisch
Lehrinhalt

Selection of topics for Upstream processing

- medium/supplements
- cultivation modes and feeding strategies
- up scaling strategies
- cultivation systems for anchorage dependent cells (e.g. stem cells)
- single use equipment and disposables
- examples of different production systems/bioreactors/process modes for biotechnological and biopharmaceutical products incl. modern cell based therapy products

Downstream Processing (Buyel)
1Introduction – Connections to bioengineering, adaptive content
2Media and supplements
3Cultivation scale up
4Adherent cells
5Adherent cell reactors
6Disposable technologies
7Coagulation: Flocculation, precipitation, and crystallization
8Liquid extraction
9Refolding
10Chromatography – affinity tags, continuous operation
11Clearance of DNA and viruses
Inhaltliche Voraussetzungen (erwartete Kenntnisse)

Basic chemical engineering knowledge is requested. This includes basics in heat, mass, momentum transfer, and basics in thermodynamics. Solutions of differential equations (ODE solver). Furthermore basic knowledge in bioporocess engineering and cell culture technology as well as cell biology is desirable
Lehrziel

After successful completion of this course, students are able:
-to select suitable medium composition and supplements, choice of production host, select suitable bioreactor, cultivation mode and feeding strategy as well as unit operations for a given biotechnological production problem, such as manufacturing of bioactive compounds/cell based therapeutics.
-to select appropriate unit operations as well as quality and in-process control methods and standards for biotechnological manufacturing.
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.