�GMBT 2024
Michael Baumschabl received the OeGMBT PhD Award 2024
Congratulations to Michael Baumschabl, who has been awarded the prestigious PhD Award at this year’s ÖGMBT conference for his outstanding dissertation. His research significantly improved the synthetic autotrophic strain Komagataella phaffii, which uses CO2 as its sole carbon source, enabling it to produce up to 600 mg/L of lactic acid. Additionally, he discovered a natural recycling pathway for the toxic byproduct 2-phosphoglycolate through the deletion of the CYB2 gene.
From natural to synthetic: Promoter engineering in yeast expression systems
Nguyen, L; Schmelzer, B; Wilkinson, St; Mattanovich D. 2024 Biotechnol Adv. 2024 Sep 7:77:108446. doi: 10.1016/j.biotechadv.2024.108446.
Single carbon metabolism - A new paradigm for microbial bioprocesses?
Baumschabl M, Ata Ö, Mattanovich D.2024 - Synth Syst Biotechnol. 2024 Mar 11;9(2):322-329. doi: 10.1016/j.synbio.2024.03.003. PMID: 38545459; PMCID: PMC10965485.

Fermenting the future – on the benefits of a bioart collaboration

In this article Diethard Mattanovich, Martin Altvater, Özge Ata and Simone Bachleitner explore the intersection of science and art through a collaboration between us scientists and the bioartists Anna Dimitriu and Alex May, focusing on the interface of yeast biotechnology and art. FEMS Yeast Research, Volume 24, 2024, foae004, https://doi.org/10.1093/femsyr/foae004
Fermenting Futures project is exhibited in “Fungi: In Art and Science” at the at the Nobel Prize Museum Stockholm
from 30th September 2023 – 7th January 2024 the project "Fermenting Futures" of Anna Dumitriu and Alex May, created in cooperation with acib and our research group, will be part of the autumn exhibition "Fungi – In Art and Science", the world of fungi is explored through artworks, design objects, fashion and current scientific research.
Hefe statt Rohöl – auf dem Weg zu erneuerbaren Kunststoffen
Vor dem Hintergrund des Klimawandels wird Recycling von CO2 immer interessanter. Özge Ata und Michael Baumschabl aus dem Lab von Diethard Mattanovich und Wissenschafter der acib GmbH konnten eine CO2 produzierende, heterotrophe Hefe so modifizieren, dass sie ihre Biomasse gänzlich aus CO2 aufbauen kann. Ihre Forschungsarbeit wurde aktuell in PNAS veröffentlicht. doi.org/10.1073/pnas.2211827119
Into the metabolic wild: Unveiling hidden pathways of microbial metabolism.
Ata Ö, Mattanovich D. 2024 - Microb Biotechnol. 2024 Aug;17(8):e14548. doi: 10.1111/1751-7915.14548. PMID: 39126421; PMCID: PMC11316390.
Efficient production of itaconic acid from the single-carbon substrate methanol with engineered Komagataella phaffii
Severinsen MM, Bachleitner S, Modenese V, Ata Ö, Mattanovich D. 2024 - Biotechnol Biofuels Bioprod. 2024 Jul 15;17(1):98. doi: 10.1186/s13068-024-02541-1. PMID: 39010147; PMCID: PMC11251334.

A native phosphoglycolate salvage pathway of the synthetic autotrophic yeast Komagataella phaffii

Baumschabl, M; Mitic BM; Troyer, C; Hann, S; Ata, Ö; Mattanovich D. (2024). Microlife. 2023 Dec 11:5:uqad046. doi: 10.1093/femsml/uqad046. eCollection 2024.

Carbon efficient production of chemicals with yeasts

Vásquez Castro, E; Memari, G; Ata, Ö; Mattanovich, D. (2023) Yeast. 2023 Dec;40(12):583-593. doi: 10.1002/yea.3909. Epub 2023 Nov 23.
Customizing amino acid metabolism of Pichia pastoris for recombinant protein production.
Rußmayer H, Buchetics M, Mattanovich M, Neubauer S, Steiger M, Graf AB, Koellensperger G, Hann S, Sauer M, Gasser B, Mattanovich D. Biotechnol J. 2023 Sep 5:e2300033. doi: 10.1002/biot.202300033. Online ahead of print. PMID: 37668396
Process engineering towards an oxidative cellular state improves 3-hydroxypropionic acid production with Lentilactobacillus diolivorans.
Russmayer H, Ergoth S, Marx H, Sauer M. Bioresour Technol. 2023 Aug;382:129160. doi: 10.1016/j.biortech.2023.129160. Epub 2023 May 11. PMID: 37178779

Umschwung für die chemische Industrie: VIVALDI verwandelt CO2-Emissionen in nachhaltige Bioprodukte

Die Europäische Union hat 7 Millionen Euro für das VIVALDI-Projekt bewilligt, um die biobasierte Industrie in einen neuen, umweltfreundlicheren und wettbewerbsfähigeren Sektor zu verwandeln. Die BOKU erhielt dabei den zweitgrößten Anteil am Projektvolumen.

Overexpression of the transcriptional activators Mxr1 and Mit1 enhances lactic acid production on methanol in Komagataellaphaffii
Bachleitner S, Severinsen MM, Lutz G, Mattanovich D. 2024 - Metab Eng. 2024 Sep;85:133-144. doi: 10.1016/j.ymben.2024.07.013. Epub 2024 Jul 25. PMID: 39067842.

The oxygen-tolerant reductive glycine pathway assimilates methanol, formate and CO2 in the yeast Komagataella phaffii

Mitic, BM; Troyer, C; Lutz, L; Baumschabl, M; Hann, S; Mattanovich, D. (2023) Nat Commun. 2023 Nov 27;14(1):7754. doi: 10.1038/s41467-023-43610-7.
Tailored extraction and ion mobility-mass spectrometry enables isotopologue analysis of tetrahydrofolate vitamers.
Mitic BM, Mattanovich D, Hann S, Causon T. Anal Bioanal Chem. 2023 Sep;415(21):5151-5163. doi: 10.1007/s00216-023-04786-5. Epub 2023 Jun 22. PMID: 37347300
From strain engineering to process development: monoclonal antibody production with an unnatural amino acid in Pichia pastoris
Nora Tir, Lina Heistinger, Clemens Grünwald-Gruber, Leo A Jakob, Stephan Dickgiesser , Nicolas Rasche and Diethard Mattanovich published their results on how Pichia pastoris was successfully employed for cost-effective laboratory-scale production of a monoclonal antibody with an unnatural amino acid. Microb Cell Fact. 2022 Aug 11;21(1):157. doi:10.1186/s12934-022-01882-6.

Adaptive laboratory evolution and reverse engineering enhances autotrophic growth in Pichia pastoris

The engineered P. pastoris strains are a basis for the development of a platform technology, which uses CO2 for production of value-added products, such as cellular biomass, technical enzymes and chemicals and which further avoids consumption of organic feedstocks with alternative use in food production. Metab Eng. 2022 Jan;69:112-121. doi: 10.1016/j.ymben.2021.11.007.

Christian Doppler Laboratory for Growth-Decoupled Protein Production in Yeast

A report on the CD-lab of Brigitte Gasser in the magazine "Chemiereport 2017-03" (text only available in German).