By this technique, almost all variants of liposomes and lipid nanoparticles can be produced.
In figure 2 classification of liposomes and the surface modification strategies applied in each category. (A) Conventional liposomes simply contain: neutral, anionic, and cationic phospholipids; (B) stealth liposomes are PEGylated contain a polyethylene glycol (PEG) layer; (C) multifunctional liposomes have modified surfaces in addition to carrying imaging agent for diagnostic purposes (diagnosis and treatment functions); and, (D) targeted liposomes have modified surfaces through the attachment of targeting ligands (antibody, protein, peptide, small molecule, carbohydrate) [Trang Le et.al; DOI: 10.3390/ijms20194706]
Scalable technologies are still quite rare. In particular, for large and sensitive molecules, such as proteins and DNA/RNA. Within an industrial collaboration with Polymun Scientific, Klosterneuburg, Austria, we developed a promising, meanwhile patented, technique. The so-called Cross Flow Injection Technique (CFIT) is convenient to overcome known limitations. Relevant achievements are shown in table 1.