Cell isolation form different donor tissue (e.g. umbilical cord, adipose tissue, bone marrow)

Advanced 3 D techniques for stem cell isolation

Cultivation of cell under hypoxic conditions

Application of dynamic (3 D) culture conditions incl. mechanical stimulation (e.g. shear stress, straining/loading)

Characterization and functional testing of stem cells

Biomaterial testing

Co- culture systems

Integration of sensors in tailor made cultivation systems for monitoring cell culture processes (e.g. oxygen, pH, glucose)

Equipment and Resources

3 fully furnished cell culture laboratories (ca. 150 m²)

4 sterile workbenches

5 incubators with 2 of them allowing for hypoxic conditions

4 additional specialized incubation systems for the operation of bioreactors (dynamic cultivation including mechanical stimulation) equipped with various sensors and a flow-through microscope

Various bioreactors systems for cell expansion (e.g. stirred tank, hollow fibre, vertical wheel) and differentiation (e.g. perfusion, straining/hydrostatic pressure)

3 transmission light microscopes, a fluorescence microscope, live-cell-imaging (JuLi)

Additional laboratory space for standard microbiological analytics:

plate-reader, PCR/ qPCR, NanoDrop, DNA/SDS-gel-electrophoreses, western blot, HPLC, photometer, Bioanalyzer, flow cytometry, Pre-Sense sensor systems for O2 and pH measurements, glucose/lactate analyser (YSI).

Additionally, access to various Core Facilities: TEM, REM, confocal microscopy, cell sorting, nanoparticle tracking analysis (NTA, Zeta View) device, etc.

Excellent collaboration with the Universitätsklinikum Tulln, the Vienna General Hospital, the Medical University Graz and the Agaplesion Diakonie Klinikum Hamburg ensures supply with primary mesenchymal stem cells from donor tissue.

Collaboration with University Vienna and Technical University Vienna for Proteomics analyses

Collaboration with Veterinary School Vienna (VetCore, Anatomy) for µCT/nano-CT and specialized microscopic techniques, Histology

Collaboration with Danube University Krems for extracellular vesicles characterization