Core Facility Biomolecular & Cellular Analysis

DUE TO THE COVID-19 PANDEMIC WE ARE OPERATING IN A RESTRICTED MODE!

Currently, we are allowed to have 2 Users simultaneously working in the lab (room B01-015, "big lab"). Our safety rules have to be followed strictly (lab coat, mask, gloves, hand desinfection and distance of 2 m to another person).

Also for external users (academia and industry) unrestricted booking of our instrumentation is possible again.

For the flow cytometry room (room B01-021), 2 people are allowed at a time BUT FFP2-masks have to be worn continuously (provided by Core Facility, located next to Sony Sorter). If a User is working alone, of course a normal mouth-nose-mask is sufficient. Please make sure to use desinfectant for your hands and the surfaces before and after usage of an instrument! Both, the 2-laser and the 4-laser CytoFLEX are operating again.

For all questions, please email to bmca(at)boku.ac.at!

Biomolecular analysis

A strong focus lies on the investigation and characterization of all kinds of biomolecular interactions. For that purpose we are equipped with:

• Surface plasmon resonance spectrometer (Biacore T200, GE Healthcare)
• Biolayer interferometer (Octet RED96e, Pall)
• Isothermal titration calorimeter (PEAQ-ITC, Malvern Panalytical)

For characterization of biomolecules in terms of thermal stability, thermodynamics of unfolding, aggregation behaviour, size and zeta potential, the following equipment is available:

• Differential scanning calorimeter (PEAQ-DSC, Malvern Panalytical)
• Dynamic light scattering – cuvette mode (Zetasizer Nano ZSP with Autotitrator, Malvern Panalytical)

Cellular analysis

As our name implies, we also offer techniques for the analysis of cells or cellular components. This part is mainly covered by flow cytometry:

• Flow cytometry – analysis (CytoFLEX S, Beckman Coulter)
• Flow cytometry – cell sorting (SH800S, Sony)
• Flow cytometry – cell sorting (BC MoFlow Astrios EQ, Beckman Coulter)

Further, there is the possibility to study the genotoxic potential of compounds on adherent and suspension mammalian cell culture:

• Genotoxicity (gTOXXs Analyzer, 3T Analytik)

Last but not least, it is possible to measure size distribution and zeta potential of primarily extracellular vesicles using nano particle tracking analysis (ZetaView, particle-metrix):

• Nanoparticle tracking analysis NTA (ZetaView, particle-metrix)