Core Facility Biomolecular & Cellular Analysis

The BOKU Core Facility Biomolecular & Cellular Analysis (BmCA) is a state-of-the-art analytical laboratory with focus on biomolecular characterization and characterization of biomolecular interactions, protein crystallography as well as flow cytometry.

Beside high-end instrumentation, we offer the possibility to prepare your samples in the most optimal way. We provide basic laboratory equipment and we offer two PC work stations which are accessible for all Core Facility users to utilize the (licenced) special software of the various instruments.

Besides providing instruments, we offer professional training to the certain techniques as well as consultation regarding experimental setup and design. Further, it is possible to make use of our full-service, which comprises the measurements of your samples by trained staff and the generation of experimental reports.

Biomolecular analysis

A strong focus lies on the investigation and characterization of all kinds of biomolecular interactions. For that purpose, we are equipped with:

For characterization of biomolecules in terms of molecular weight, thermal stability, thermodynamics of unfolding, aggregation behaviour, size and zeta potential, the following equipment is available:

For purification of protein samples, you can book our:

Protein purification (AEKTA go, Cytiva)

Cellular analysis

As our name implies, we also offer techniques for the analysis of cells or cellular components. This part is mainly covered by flow cytometry:

Further, there is the possibility to measure size distribution and zeta potential of primarily extracellular vesicles using nano particle tracking analysis (ZetaView, particle-metrix):

Nanoparticle tracking analysis NTA (ZetaView, particle-metrix)

Protein crystallization

The new area in our Core Facility is protein X-ray crystallography. We provide state-of-the-art infrastructure for the crystallization of proteins and subsequent analysis. For that purpose, we are equipped with:

We also provide tools and equipment for crystal harvesting, freezing, storage and dry shippers for sending crystals to the synchrotron. Shipment of crystals and data collection is part of our service.