Bioautography is a powerful analytical technique that combines chromatography and biological assays to identify bioactive compounds in complex mixtures. By separating compounds on a chromatographic plate and overlaying it with a microbial or enzymatic medium, bioautography allows for the visualization of biologically active substances directly on the plate.
Our method specifically involves overlaying the plate with Bacillus subtilis and Escherichia coli, enabling the detection of compounds with antibacterial activity against these model organisms. We utilize our state-of-the-art CAMAG HPTLC system to ensure precise and high-resolution separation of compounds, providing a robust platform for bioautography. In addition, further characterization of the bioactive molecules becomes possible, as we can elute the areas of interest directly from the plate and analyze them using mass spectrometry (MS).
This comprehensive approach is widely used in natural product research, drug discovery, and antimicrobial studies to pinpoint and characterize compounds with specific biological activities.
