About the instrument

The ImageXpress® Micro Widefield High Content Screening system from Molecular Devices is an integrated cellular imaging and analysis system that is designed for rapid, automated screening of fluorescently labeled biological samples in microplates.

The core hardware component of the imaging systems is a custom-designed, fully automated, epi-fluorescence microscope, with rapid autofocus and precision sample movement features that allow large numbers of high-resolution images to be acquired in the shortest possible time. All key optical and mechanical elements are motorized, with asynchronous command execution, thereby allowing complete real-time control of the instrument configuration through the MetaXpress High Content Image Acquisition and Analysis Sofwtare intreface. 

WARNING! Due to camera failures, these systems are currently not bookable.

Technical details

  • External solid-state light source: LUMENCOR Light Engine (380 nm - 680 nm) connected by a liquid light guide
  • Motorized sample satge (X-Y), 
  • Z-focus: motorized;
  • 5-position filter cube changer with Semrock filters
  • 4-position objective changer (only the selected objective moves up and down in Z)
  • Detector: Andor Zyla 5.5 CL1021/E sCMOS camera

Nikon objectives for both devices (default set):

  • Plan Apo 4x/0.020  Dry,  WD: 15.7 mm,   
  • Plan Fluor 10x/0.030, Dry WD: 16.0 mm,  
  • S Fluor 10x/0.050 Dry,  WD: 1.2 mm,  
  • S Fluor 20x/0.75 Dry,  WD: 1.0 mm (compatible with thin bottom (0.17 mm) plates, only!)

Filters on the first device (ImageXpress MD1):

  • TexasRed
  • GFP
  • Cy5
  • YFP
  • FRET

Filters on the second device (ImageXpress MD2)

  • DAPI
  • GFP
  • Cy5 (narrow)
  • TexasRed
  • FRET (CFP/YFP)

These two high content screening devices (ImageXpress MD1 and ImageXpress MD2) can be booked separately!