About the instrument

The Leica LMD6500 laser microdissection system serves as a technology for precision sample preparation which is a prerequisite of various downstream experiments in the fields of genomics, transcriptomics, proteomics, etc. … The LMD microscope prepares samples directly from tissue sections or cell cultures using a UV laser (355 nm) as an ultra-sharp knife to separate the objects of interest from the surrounding matrices. The method is contact- and contamination-free, one can isolate specific single cells or entire areas of tissues. The dissectate is then collected by gravity in small tubes (PCR, Eppendorf) and available for further analytical techniques,

https://www.leica-microsystems.com/solutions/life-science/laser-microdissection/

Technical details

  • Microscope stand: DM6000B, upright configuration
  • Stage: motorized, inserts for slides and cell culture dishes (30 mm and 50 mm Ø)
  • Z-focus: motorized;
  • Detector: CCD camera (DFC310FX)
  • Laser: 355 nm (pulse freq.: 80 Hz, max. pulse energy: 50 µJ)

Objectives (default set):

  • UVI   6.3x/0.13 CS,  Dry,    (11518145)
  • HCX PL FL  10x/0.30   Dry,   PH1,  -/D 11.0   (11506507)
  • HCX  PL FL L  20x/0.40  Dry,  PH1,  0 -2 / C  (11506243)        
  • HCX  PL FL L   40x/0.60  Dry,  PH2,  XT,   (11506209)
  • HCX  PL FL L  63x/0.70  Dry,   PH2   (11506217)
  • HCX  PL FL   150x/0.90   0/C  0.25   (11506214)