TCS SP5 confocal microscope

The Leica TCS SP5 is a laser (point) scanning confocal microscope that enables imaging of transparent, medium thick samples via optical sectioning. Specimens/objects of interest should be fluorescently labelled for image acquisition.

Labelling protocols should be adapted to the existing laser lines that can excite the reporter dye molecules or fluorescent proteins.

• Microscope stand: DM6000, upright configuration
• Scanning head: SP5 II with Acousto-Optical Beam Splitter (AOBS) and resonant scanner (8 kHz)
• Stage: motorized, inserts for slides, Petri dishes and microtiter plates
• Detectors: 2 HyDs, 3 PMTs + TLD
• Lasers:
  405 nm (cw 50 mW)
  Argon laser (5 lines)
  561 nm
  633 nm

Objectives (default set):

• HC PL APO CS  10x/0.40  Dry,  WD: 2.2,  DIC,  15506285)
• HC PL APO CS  20x/0.70 ImmCorr,  WD: 0.26-0.17,  CCorr.  DIC,  (15506326)
• HCX PL APO CS  40x/0.85  Dry,  WD: 0.21,  CCorr.  (15506295)
• HCX PL APO CS  63x/1.4  Oil,  WD: 0.1,  (15506188)
• HCX PL APO CS  63x/1.30 Glyc,   WD: 0.28,  CCorr.  (15506194)
• HCX  APO L  40x/0.80  Water,  WD: 3.3,  UV - V - I   (15506155)
• HCX  APO L  63x/0.90  Water,  WD: 2.2,  UV - V - I   (15506148)

Visual observation of the fluorescent signal is possible by illuminating the samples with a metal halide lamp, however, the stand holds no camera for non-confocal image acquisition.

LAS Software Modules:

• Live Data Mode
• FRAP Wizard
• FRET Wizard