Arbeits- und Forschungsfeld

Das Interesse an der biologischen Funktion der Protein-Glykosylierung – eines Phänomens, welches fast alle Proteine im Serum und auf Zelloberflächen betrifft – bedingt das Vorhandensein einer leistungsfähigen Analytik in Form der Flüssigchromatographie-Massenspektometrie (LC-MS). So diese nun erfreulicherweise zur Verfügung steht, kann sie für vielfältigste Aufgaben der Protein- sowie eben auch Glykoprotein-Analyse eingesetzt werden.

Vielleicht eine gute Stelle um darauf zu verweisen, dass der zehnt-meistzitierte Biologe/Biochemiker Österreichs an der BOKU tätig ist.

 

Zu den Forschungsbereichen

Aktuelle Themen für Diplomarbeiten (aka Masterarbeiten)

Beispiele für mögliche Themen:

-  Herstellung neuartiger Glyco-Vakzine
-  Isomer-spezifische massenspektroskopische Glykan Analytik
-  Aufklärung der Struktur der neuartigen N-Glykane essbarer Algen
-  Glykoproteine in der Allergiediagnostik

-  Strategies in glycoproteome analysis
 

Anfragen an j.stadlmann@boku.ac.at  oder  friedrich.altmann@boku.ac.at *

* Hier wäre allerdings anzumerken, dass F. A. seit März 2022 keine Betreuungen mehr übernehmen darf um Demokratisierung, Meinungsfreiheit und Diskriminierungsfreiheit hier nicht weiter zu gefährden.

Ausgewählte Arbeiten aus der AG Altmann

Helm J, Hirtler L, Altmann F. (2022) Towards Mapping of the Human Brain N-Glycome with Standardized Graphitic Carbon Chromatography. Biomolecules. 12(1):85.

Stenitzer D, Mócsai R, Zechmeister H, Reski R, Decker EL, Altmann F. (2022) O-methylated N-glycans Distinguish Mosses from Vascular Plants. Biomolecules 12(1):136. doi: 10.3390/biom12010136.

Mócsai R, Göritzer K, Stenitzer D, Maresch D, Strasser R, Altmann F. (2021) Prolyl Hydroxylase Paralogs in Nicotiana benthamiana Show High Similarity With Regard to Substrate Specificity. Front Plant Sci. 12. 636597.

Mócsai R, Kaehlig H, Blaukopf M, Stadlmann J, Kosma P, Altmann F. (2021) The Structural Difference of Isobaric N-Glycans of Two Microalgae Samples Reveals Taxonomic Distance. Front Plant Sci. 2021 Apr 26;12:643249.

Helm J, Grünwald-Gruber C, Thader A, Urteil J, Führer J, Stenitzer D, Maresch D, Neumann L, Pabst M, Altmann F. (2021) Bisecting Lewis X in Hybrid-Type N-Glycans of Human Brain Revealed by Deep Structural Glycomics. Anal Chem. 93, 15175-15182

Mócsai R, Blaukopf M, Svehla E, Kosma P, Altmann F. (2020) The N-glycans of Chlorella sorokiniana and a related strain contain arabinose but have strikingly different structures. Glycobiology 30, 663-676.

Mócsai R, Figl R, Sützl L, Fluch S, Altmann F. (2020) A first view on the unsuspected intra-genus diversity of N-glycans in Chlorella microalgae. Plant J. 103, 184-196.

Mócsai R, Figl R, Troschl C, Strasser R, Svehla E, Windwarder M, Thader A, Altmann F. (2018) N-glycans of the microalga Chlorella vulgaris are of the oligomannosidic type but highly methylated.  Sci Rep. 9(1):331. doi: 10.1038/s41598-018-36884-1.

Grünwald-Gruber C, Thader A, Maresch D, Dalik T, Altmann F (2017) Determination of true ratios of different N-glycan structures in electrospray ionization-mass spectrometry. Anal. Bioanal. Chem . 409(10):2519-2530   doi: 10.1007/s00216-017-0235-8.

Maresch D, Altmann F. (2016) Isotype-specific glycosylation analysis of mouse immunoglobulin G by liquid chromatography-mass spectrometry. Proteomics. 16, 1321-1330.  doi: 10.1002/pmic.201500367.

Windwarder M, Figl R, Svehla E, Mócsai RT, Farcet JB, Staudacher E, Kosma P, Altmann F. (2016) "Hypermethylation" of anthranilic acid-labeled sugars confers the selectivity required for liquid chromatography-mass spectrometry. Anal Biochem 514, 24-31   doi: 10.1016/j.ab.2016.09.008

Windwarder M, Altmann F. (2014) Site-specific analysis of the O-glycosylation of bovine fetuin by electron-transfer dissociation mass spectrometry. J Proteomics 108,  258-268

Holzweber F., Svehla E., Fellner W., Dalik T., Stubler S.,  Hemmer W., Altmann F. (2013) Inhibition of IgE binding to cross-reactive carbohydrate determinants enhances diagnostic selectivity. Allergy 68, 1269-1277.

Pabst M., Chang M., Stadlmann J., Altmann F. (2012) Glycan profiles of the 27 N-glycosylation sites of the HIV envelope protein CN54gp140. Biol. Chem. 393, 719–730

Pabst M.,  Grass J., Tögel S., Liebminger E., Strasser R., Altmann F. (2012)  Isomeric analysis of oligomannosidic N-glycans and their dolichol-linked precursors. Glycobiology, 22, 389-399

Grass J., Pabst M., Chang M., Wozny M., Altmann F. (2011) Analysis of recombinant human follicle-stimulating hormone (FSH) by mass spectrometric approaches. Anal. Bioanal. Chem. 400, 2427-2438

Pabst M., Grass J., Fischl R., Léonard R., Jin C., Hinterkörner G., Borth N., Altmann F. (2010) Nucleotide and Sugar Nucleotide Analysis by LC-ESI-MS on Surface-Conditioned Porous Graphitic Carbon. Anal. Chem. 82, 9782-9788.

Stadlmann J, Pabst M, Altmann F. (2010) Analytical and Functional Aspects of Antibody Sialylation.  J. Clin. Immunol. 30, S15-S19.

Stadlmann J., Weber A., Pabst M., Anderle H., Kunert R., Ehrlich H., Schwarz Hans P., Altmann F. (2009) A close look at human immunoglobulin G sialylation and subclass distribution after lectin fractionation. Proteomics 9, 4143-4153

Jin C., Hantusch G., Hemmer W., Stadlmann J., Altmann F. (2008) Affinity of IgE and IgG against cross-reactive carbohydrate determinants on plant and insect glycoproteins. J. Allergy Clin. Immunol. 121, 185-190

Stadlmann J., Pabst M., Kolarich D., Kunert R., Altmann F., (2008) Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides. Proteomics 8, 2858-2871

Leonard R., Martin P., Bondili J.S., Chambat G., Veit C., Strasser R., Altmann F. (2008) Identification of the Arabidopsis gene encoding the GH95 α1,2-fucosidase active on xyloglucans. Phytochemistry 69, 1983-1988

Pabst, M., Altmann F. (2008) Influence of electrosorption, solvent, temperature and ion polarity on the performance of LC-ESI-MS using graphitic carbon for acidic oligosaccharides. Anal. Chem. 80, 7534-7542

Altmann F. (2007) The role of protein-glycosylation in allergy. Int. Arch. Allergy Immunol. 142, 99-115

Pabst M., Bondili J.S., Stadlmann J., Mach L., Altmann F. (2007) Mass + retention time = structure: A strategy for the analysis of N-glycans by carbon LC-ESI-MS and its application to fibrin N-glycans. Anal. Chem. 79, 5051-5057

Jin C., Bencúrová B., Borth N., Ferko B., Jensen- Jarolim E., Altmann F., Hantusch B. (2006)  Immunoglobulin G specifically binding plant N-glycans with high affinity could be generated in rabbits but not in mice. Glycobiology 16, 349-357

Léonard D., Rendić D., Rabouille C., Wilson I.B.H., Préat H., Altmann F. (2006) The Drosophila fused lobes gene encodes an N-acetylglucosaminidase involved in N-glycan processing. J. Biol. Chem. 281, 4867-4875

Zeleny R., Léonard R., Dorfner G., Dalik T., Kolarich D., Altmann F. (2006) Molecular cloning and characterisation of a plant α1,3/4-fucosidase based on sequence tags from almond fucosidase I. Phytochemistry 67, 641-648

Leonard R., Petersen B.O., Himly M., Kaar W., Wopfner N., Kolarich D., van Ree R., Ebner C., Duus J.,  Ferreira F., Altmann F.  (2005) Two novel types of O-glycans on the mugwort pollen allergen Art v 1 and their role in antibody binding. J. Biol. Chem. 280, 7932-7940

Bencurova M., Hemmer, W., Focke-Tejkl M., Wilson I.B.H., Altmann F. (2004) Specificity of IgG and IgE antibodies against plant and insect glycoprotein glycans determined with artificial glycoforms of human transferrin. Glycobiology 14, 457-466.  

Wilson I.B.H., Zeleny R., Kolarich D., Staudacher E., Stroop C.J.M., Kamerling J.P. and Altmann F. (2001) Analysis of Asn-linked glycans from vegetable foodstuffs:  Widespread occurrence  of Lewis a, core a1,3‑linked fucose and xylose substitutions. Glycobiology 11, 261-274

Leiter H., Mucha I., Staudacher E., Grimm R., Glössl J., Altmann F. (1999) Purification, cDNA cloning and expression of GDP-l-Fuc : Asn-linked GlcNAc a1,3-fucosyltransferase from mung beans. J. Biol. Chem. 274, 21830-21839

Altmann F., Schwihla H., Staudacher E., Glössl J., März L. (1995) Insect cells contain an unusual, membrane-bound beta-N-acetylglucosaminidase probably involved in the processing of protein N-glycans. J. Biol. Chem. 270, 17344-17349

Kubelka V., Altmann F., Staudacher E., Tretter V., März L., Hård K., Kamerling J.P., Vliegenthart J.F.G. (1993) Primary structures of the N-linked carbohydrate chains from honeybee venom phospholipase A2. Eur. J. Biochem. 213, 1193-1204

Tretter V., Altmann F., Kubelka V., März L., Becker W.M. (1993) Fucose linked 1-3 to the core-region of glycoprotein N-glycans creates an important epitope for IgE from honeybee venom allergic individuals. Int. Arch. Allergy Immunol. 102, 259-266 103      

Tretter V., Altmann F., März L. (1991) Peptide-N4-( N-acetyl-beta-glucosaminyl asparagine amidase F cannot release glycans with fucose attached alpha 1->3 to the asparagine-linked N-acetyl-glucosamine residue.  Eur. J. Biochem. 199, 647-652