About the instrument

The Horiba XploRATM INV Raman is a fully motorized confocal microscope that allows fast non-destructive chemical micro-analysis and automated high definition Raman chemical imaging. The instrument comprises a microscope and a spectrometer built together; this combination enables the acquisition of a complete Raman spectrum at each and every pixel of the image, and then it generates a false colour image based on material composition, phase, crystallinity and strain. 

In standard operation this microscope – as any other conventional light microscope -, is diffraction limited, with achievable spatial resolution in the order of 250-300 nm (depending on laser wavelength and objective in use). Features smaller than this can still be detected but without true spatial information.    

Standard point-by-point mapping affords the ultimate sensitivity for materials with extremely low Raman scattering properties, and allows high resolution, large spectral range capability but total measurement times can be significant.

Technical details

  • Microscope stand: Nikon Eclipse TiU (inverted)
  • Stage: motorized, equipped with Okolab UNO PLUS incubation system
  • Lasers:
    532 nm (Solid State; 100mW)
    785 nm (Diode; 90 mW)
  • NDD set: 100%, 50%, 25%, 10%, 1%, 0.1%
  • Gratings: 600 gr/mm, 1200 gr/mm, 1800 gr/mm, 2400 gr/mm
  • Detector: EMCCD (TE cooled); 1600 x 200 pixel; front illuminated

Objectives (default set):

  • Plan Fluor EPI  5x/0.15  Dry, WD=23.5 mm   (ZK-5XVIS)
  • Plan Fluor  10x/0.3  Dry, WD=15.2 mm   (10X-INV-TiU)
  • Apo Lambda S  40x/1.15  Water,  CORR WD=0.6 mm  (ZK-40XLWD IM-W-TiU)
  • CFI Plan APO  100x/1.4 Oil,   WD=0.13 mm  (ZK-100XIM-ÖL-TiU)
    + Objective for Auto-calibration (all gratings + all laser lines)

Special features

The SWIFT TM Ultra-fast module offers drastically reduced measurement times allowing large area mapping, 3D confocal volume mapping and detailed Raman images (acquisition time <5ms/point) to be completed within seconds or minutes.

The DuoScan TM technology enables the ultimate flexibility for Raman mapping, including sub-micron Raman imaging, adjustable laser spot size, and 100% coverage of a sample for macro-mapping.