Post Doc Position on Promoter Engineering of Pichia pastoris for Customised Protein Production

Research field

Yeasts are valuable hosts for production of heterologous proteins. The aim of this project is to identify and engineer promoters of the methylotrophic yeast Pichia pastoris that are specific growth conditions in bioreactor cultures, and to validate their performance.

Within this project we aim to develop a P. pastoris expression platform that is specifically designed for its performance in non-standard bioreactor setups and cultivation conditions. Gene expression data will be used to identify suitable promoters, which will be further engineered based on computational analysis of their structure. Performance of the newly designed promoters will be evaluated in bioreactors and the best performing will be implemented in the expression platform.

Research will take place in the group “Metabolic and Cell Engineering” at the Institute of Microbiology and Microbial Biotechnology at the University of Natural Resources and Life Sciences Vienna (BOKU).

Requirements

  • Completed Master and PhD degree (or equivalent) in biotechnology, biochemical engineering, molecular biology, genetics, biochemistry, or similar study.
  • Strong background in synthetic biology, molecular biology, yeast metabolism, biochemical engineering or related disciplines.
  • Strong practical experience in molecular biology, biochemistry and microbiology requested, and scientific interest in bioreactor cultivation desired.
  • Excellent skills in spoken and written English, an organized approach with strong attention to detail and good communication skills with ability to work well in teams.

Application deadline extended until: 20.04.2021       Start: May - June 2021 

Please apply by email to birgit.marckhgott(a)boku.ac.at  with a single PDF file containing: Ref. No. #01_2021

* a detailed CV and publication list, * a motivation letter, * copies of university degree documents, * contact information (phone number and email) and *contact details of up to  3 academic referees

→ download this job advertisement as pdf-File

Post Doc Position on Metabolic Engineering of Pichia pastoris for the Valorisation of CO2 to Chemicals

Research field

Yeasts are valuable chassis organisms for synthetic biology applications towards sustainable production of biobased chemicals. A special interest is focused on methylotrophic yeasts for the conversion of single carbon substrates such as methanol, formate and CO2

Within the EU-funded project VIVALDI (Innovative bio-based chains for CO2 valorisation as added-value organic acids) that is dedicated to the valorisation of industrial CO2 emissions for fighting climate change and adopting circular economy, we are focusing on designing novel yeast strains that can convert a mix of C1-substrates to organic acids.

Research will take place in the group “Metabolic and Cell Engineering” at the Institute of Microbiology and Microbial Biotechnology at the University of Natural Resources and Life Sciences Vienna (BOKU).

Requirements

  • Completed Master and PhD degree (or equivalent) in biotechnology, biochemical engineering, molecular biology, genetics, biochemistry, or similar study.
  • Strong background in synthetic biology, microbial metabolism, metabolic engineering incl. related disciplines.
  • Strong practical experience in molecular biology, metabolic engineering and/or synthetic biology requested, and scientific interest in metabolic modelling and bioreactor cultivation desired.
  • Excellent skills in spoken and written English, an organized approach with strong attention to detail and good communication skills with ability to work well in teams.

Application deadline extended until: 20.04.2021         Start: June - July 2021 

Please apply by email to birgit.marckhgott(a)boku.ac.at  with a single PDF file containing: Reference No: #02_2021,
* a detailed CV and publication list, * a motivation letter, * copies of university degree documents, * contact information (phone number and email) and * contact details of up to 3 academic referees

Contact: Prof. Dr. Diethard MATTANOVICH

→ download this job advertisement as pdf-File

Bachelor or Diploma/Master Thesis Positions

Bachelor and master thesis projects are available upon request.

If you are interested in our research and would like to do your thesis in our lab please contact us!