Liquid chromatography combined with tandem mass spectrometry offers sensitive, fast, and robust quantification of a variety of molecule types across a wide range of small molecule applications in the fields of metabolomics, environmental analysis and food analysis. The system allows the determination of isotopologues and isotopomer ratios in metabolic flux analysis. This system is ideal for accurate compound quantification using multiple reaction monitoring covering concentrations over six orders of magnitude.

Technical Information

TSQ Vantage Triple Quadrupole LC-MS/MS. Thermo Fisher Scientific GmbH. Obtained 2012.

The system consists of a 1200 bar Accela HPLC System for ultra-fast separations, electrospray or atmospheric pressure chemical ionization ion source options and a fast scanning MS/MS. In addition to routine reversed-phase (RPLC) and hydrophilic interaction (HILIC) methods, the system can also be coupled with an ICS5000+ Ion Chromatography system for targeted applications.